LOCUS       sequence-176086-        4506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length 410 amino acid SUV39H2 histone 
            methyltransferase linked to VP16 activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Askew EB, Bai S, Parris AB, Minges JT, Wilson EM
  TITLE     Androgen receptor regulation by histone methyltransferase Suppressor
            of variegation 3-9 homolog 2 and Melanoma antigen-A11.
  JOURNAL   Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29.
  PUBMED    28042025
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2017-03-5"; volume: "443:42-51. doi"; pages: " 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29"
COMMENT     Sequence Label: VPCT-SUV39H2-L
FEATURES             Location/Qualifiers
     source          1..4506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(124..146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     246..265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        346..364
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     346..363
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(374..394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      702..711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             714..734
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             735..968
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     polyA_signal    2361..2495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2398..2417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2452..2471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2505..2524)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2506..2524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2611..2628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2782..3370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2862..2881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3541..4332,4333..4401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4164..4183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4402..4506)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
      121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
      301 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
      361 tagaactcga tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      541 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      601 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      661 gtagtgaaga ggcttttttg gaggagatct aagctagcgc cgccaccatg ggccctaaaa
      721 agaagcgtaa agtcgccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg
      781 gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg
      841 gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc
      901 tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt
      961 acggtgggga attcccgggg atcctaatgg cggcggtcgg ggccgaggcg cgaggagctt
     1021 ggtgtgtgcc ttgcctagtt tcacttgata ctcttcagga attatgtaga aaagaaaagc
     1081 tcacatgtaa atcgattgga atcaccaaaa ggaatctaaa caattatgag gtggaatact
     1141 tgtgtgacta caaggtagta aaggatatgg aatattatct tgtaaaatgg aaaggatggc
     1201 cagattctac aaatacttgg gaacctttgc aaaatctgaa gtgcccgtta ctgcttcagc
     1261 aattctctaa tgacaagcat aattatttat ctcaggtaaa gaaaggcaaa gcaataactc
     1321 caaaagacaa taacaaaact ttgaaacctg ccattgctga gtacattgtg aagaaggcta
     1381 aacaacggat agctctgcag agatggcaag atgaactcaa cagaagaaag aatcataaag
     1441 gaatgatatt tgttgaaaat actgttgatt tagagggccc accttcagac ttctattaca
     1501 ttaacgaata caaaccagct cctggaatca gcttagtcaa tgaagctacc tttggttgtt
     1561 catgcacaga ttgcttcttt caaaaatgtt gtcctgctga agctggagtt cttttggctt
     1621 ataataaaaa ccaacaaatt aaaatcccac ctggtactcc catctatgaa tgcaactcaa
     1681 ggtgtcagtg tggtcctgat tgtcccaata ggattgtaca aaaaggcaca cagtattcgc
     1741 tttgcatctt tcgaactagc aatggacgtg gctggggtgt aaagaccctt gtgaagatta
     1801 aaagaatgag ttttgtcatg gaatatgttg gagaggtaat cacaagtgaa gaagctgaaa
     1861 gacgaggaca gttctatgac aacaagggaa tcacgtatct ctttgatctg gactatgagt
     1921 ctgatgaatt cacagtggat gcggctcgat acggcaatgt gtctcatttt gtgaatcaca
     1981 gctgtgaccc aaatcttcag gtgttcaatg ttttcattga taacctcgat actcgtcttc
     2041 cccgaatagc attgttttcc acaagaacca taaatgctgg agaagagctg acttttgatt
     2101 atcaaatgaa aggttctgga gatatatctt cagattctat tgaccacagc ccagccaaaa
     2161 agagggtcag aacagtatgt aaatgtggag ctgtgacttg cagaggttac ctcaactgac
     2221 tcgacgcgtc tgcagaagct tctagataag taatgatcat aatcagccat atcacatctg
     2281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2461 ccaaactcat caatgtatct tatcatgtct ggatctgccg gtctccctat agtgagtcgt
     2521 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     2581 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2641 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2701 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3181 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4501 tccgcg
//
