LOCUS       sequence-176090-        6027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mycobacteria shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN
  TITLE     Metabolic engineering of cofactor F420 production in Mycobacterium 
            smegmatis.
  JOURNAL   PLoS One. 2010 Dec 29;5(12):e15803.
  PUBMED    21209917
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-29"; volume: "5(12)"; pages: "e15803"
COMMENT     Sequence Label: pYUB28b-MBP
FEATURES             Location/Qualifiers
     source          1..6027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     795..814
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        795..813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    814..838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             853..875
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             897..914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2034..2054
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2061..2093
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             2142..2159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2212..2230)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2226..2273
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2310..2738
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2397..2416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2607..2628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(4841..5429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4921..4940)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtcctggcg gacgccccgg tgccggtgcc ccgcctcctc ggccgcggcg agctgcggcc
       61 cggcaccgga gcctggccgt ggccctacct ggtgatgagc cggatgaccg gcaccacctg
      121 gcggtccgcg atggacggca cgaccgaccg gaacgcgctg ctcgccctgg cccgcgaact
      181 cggccgggtg ctcggccggc tgcacagggt gccgctgacc gggaacaccg tgctcacccc
      241 ccattccgag gtcttcccgg aactgctgcg ggaacgccgc gcggcgaccg tcgaggacca
      301 ccgcgggtgg ggctacctct cgccccggct gctggaccgc ctggaggact ggctgccgga
      361 cgtggacacg ctgctggccg gccgcgaacc ccggttcgtc cacggcgacc tgcacgggac
      421 caacatcttc gtggacctgg ccgcgaccga ggtcaccggg atcgtcgact tcaccgacgt
      481 ctatgcggga gactcccgct acagcctggt gcaactgcat ctcaacgcct tccggggcga
      541 ccgcgagatc ctggccgcgc tgctcgacgg ggcgcagtgg aagcggaccg aggacttcgc
      601 ccgcgaactg ctcgccttca ccttcctgca cgacttcgag gtgttcgagg agaccccgct
      661 ggatctctcc ggcttcaccg atccggagga actggcgcag ttcctctggg ggccgccgga
      721 caccgccccc ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
      781 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
      841 tctagaaata attttgttta actttaagaa ggagatatac catggcctcc tactaccacc
      901 accaccacca ccacgacatc cccaccacca tgctcgtcat ctggatcaac ggcgacaagg
      961 gctacaacgg cctggccgaa gtgggcaaga agttcgagaa ggacaccggc atcaaggtga
     1021 cggtcgagca cccggacaag ctggaggaga agttccccca ggtcgccgcg accggcgacg
     1081 gcccggacat catcttctgg gcccacgacc gcttcggcgg ctacgcccag agcggcctgc
     1141 tcgcggagat caccccggac aaggcgttcc aggacaagct ctaccccttc acgtgggacg
     1201 ccgtgcggta caacggcaag ctgatcgcgt acccgatcgc ggtcgaggcc ctgtcgctca
     1261 tctacaacaa ggacctgctc ccgaacccgc ccaagacctg ggaggagatc cccgccctcg
     1321 acaaggagct gaaggccaag ggcaagagcg cgctcatgtt caacctgcag gagccgtact
     1381 tcacgtggcc cctgatcgcc gcggacggcg gctacgcgtt caagtacgcc gcgggcaagt
     1441 acgacatcaa ggacgtgggc gtcgacaacg ccggcgcgaa ggccggcctg accttcctcg
     1501 tggacctgat caagaacaag cacatgaacg ccgacacgga ctactccatc gcggaggccg
     1561 cgttcaacaa gggcgagacc gccatgacga tcaacggccc gtgggcgtgg tcgaacatcg
     1621 acaccagcaa ggtcaactac ggcgtgaccg tcctcccgac gttcaagggc cagccgtcga
     1681 agcccttcgt gggcgtcctg tccgccggca tcaacgccgc gtcgccgaac aaggagctcg
     1741 cgaaggagtt cctggagaac tacctgctca ccgacgaggg cctggaggcg gtgaacaagg
     1801 acaagccgct cggcgcggtc gccctgaagt cgtacgagga ggagctggcc aaggaccccc
     1861 gcatcgccgc gacgatggag aacgcgcaga agggcgagat catgccgaac atcccccaga
     1921 tgagcgcgtt ctggtacgcc gtgcgcaccg cggtcatcaa cgccgcgtcc ggccggcaga
     1981 cggtggacgc cgcgctggcg gcggcccaga ccaacggcga catccccacc acggagaacc
     2041 tctacttcca gggcgcgcat atggctagca tgactggtgg acagcaaatg ggtcgggatc
     2101 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
     2161 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     2221 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     2281 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     2341 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2401 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2461 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2521 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2581 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2641 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2701 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat ctagatttaa
     2761 agatctggta ccgcggccgc gccacggatg ccaccacagg cactacaacg gagttcgcca
     2821 cgtacatcac cacaaccacc gattctggcg gtgagctccc cgatattcag cggaaatggc
     2881 ttggtatcga ccaagattcg tagaaccccg tctcgtctgg ctggtattca aaacgggcgc
     2941 aacgaaacac gcaacgagac aggcatggcc caaaccagaa aactagcgtc taccaggact
     3001 tttacctgtc cgacccgttg caacggaacc ccccacggaa cccccgcgac acccgctccc
     3061 caattgcgtt agaacagcgg tggattgtcg gcttcgttgt gggccttttg agccgcttcc
     3121 tgttctgccg cacgctcttt cctcgcccga tagccgagtc gcttaacggt gtccagatgc
     3181 agcccgaaat gtttggccgt ttgcggccaa gagtggccct cgtcgtcgtg ataggcgcgg
     3241 atgcgttcgc ggcgtgcagc ctgctcggcg agccactcgc tgcgttcctg cgccacgagc
     3301 cggacgacgt ggcgttcgga tagtccggtg attcgagcgc cttcggcggc ggtcacgcgc
     3361 cgctttttgc ggacagtcgg ctgccggttg tagccgtcgc tgtagccgtc gctcatagca
     3421 atgcctccat gcatggctga cgcggacttt gcgcgccgcg caactgtgct cgccgccgtg
     3481 cgcgctgctg cgcccttccg cgagatggcc gactggcgcg cactgagtgt ggcctcgtag
     3541 accacgatcc cgtccgccca aatgcgcgac ttggttgtga tccaacgcca aatgctgttg
     3601 gcgatggcgc ggacctcgct gtccggtagc ggtccgggac acacgtcgtt gcacggaaat
     3661 tcggcgtttc gcgcgtggca ctcggcatag atcgcgcggc cgagtccgtc cacgttccgg
     3721 gtcggcaggt agatccgcat gagggcggga cgataggccc acaacctgac ggaatcgaac
     3781 agtgcgcaat tccgccctag cggcgtcgga gccgctttgt acgtggtctg ctgacgccag
     3841 cgcggcggtg gcatgttcgc gccgagctcg gcctcgatgt ggctgagtgt gtagagatct
     3901 gagtggagcc attccgtttc ccaggcgatg tggccggggt ttttggtcat gaggcctgag
     3961 taactgcggt cgccatcgac ggcgcgccga aggccttcgg cgcacgccgc catgtatgcg
     4021 agcggcttac gccgcgcgta ttcggtgcgt ggaacagggg cgttgagtgc ccacactgcg
     4081 tgtgcgtggc cgttggcgcg attgcccacg atcgcgttgg gcagcggatg ggacccccgg
     4141 gcgctgagcg ctcggagcgc tgcgtctgga tggtctacgt ccacgaccag caggtttgcc
     4201 agcgctgttg ggttcgcctc gatgtaccgg cggcctaggg ccgacgcgcg gctttggcgg
     4261 tagatcccct cgagcagatc gtcgcttgcc agcggccagt acggcagcca gagctgctca
     4321 aattcgtcgg cgacgtggct cacgcttggt agtagaccac gattaatcac cggtgtatgg
     4381 tccgacacga gctccaagtc agatatttcg ctgaggggcc accccacaac tgcacactcc
     4441 cccgctctcc cgtcgagccc tggtggtgga acaccagcga cagccgagca cccccaacca
     4501 cctgtaccaa ccaggaggaa cacatgcgtc gtttcgagga cgtttccggg ccgctgagag
     4561 ccgctgtggc ggccgtacac gccgccttag acccgttaga ccccctgccg cctgaatgcg
     4621 cgggtacgag ccacacagcg cccgaactta cggagctggt gggctcacgc gtgcggccgc
     4681 acgcgttgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4981 cgcttaccgg atacctgtcc gcctttctcc cctcgggaag cgtggcgctt tctcatagct
     5041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5281 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5461 acgctcatgg aactagagca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5641 tgcctgactc cccgacgtgg ccgaccagcc cgtcatcgtc aacgcctgat ccgcggtgcg
     5701 gacaggccgt gtcgcgaccg gccgtgcgga attaagccgg cccgtaccct gtgaatagag
     5761 gtccgctgtg acacaagaat ccctgttact tctcgaccgt attgattcgg atgattccta
     5821 cgcgagcctg cggaacgacc aggaattctg ggagccgctg gcccgccgag ccctggagga
     5881 gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc gagagcacca accccgtact
     5941 ggtcggcgag cccggcccgg tgatcaagct gttcggcgag cactggtgcg gtccggagag
     6001 cctcgcgtcg gagtcggagg cgtacgc
//
