LOCUS       sequence-176102-        3825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 NSP4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-NSP4SA11
FEATURES             Location/Qualifiers
     source          1..3825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(285..304)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(514..533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     633..655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(693..711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        779..883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(884..952,953..1744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1102..1121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1915..2503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2404..2423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2657..2674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2759..2777
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3673..3691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3687..3734
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(3802..3820)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3803..3820)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
       61 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
      121 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
      181 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
      241 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
      301 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
      361 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc accattcctt
      421 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcttaat gcaggagtcg
      481 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      541 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      601 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      661 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      721 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2041 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2521 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2581 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2641 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2701 accgcctctc cccgcgcgtt ggccgattca ttaatgcagg gggatatcga tcccgggtta
     2761 atacgactca ctataggctt ttaaaagttc tgttccgaga gagcgcgtgc ggaaagatgg
     2821 aaaagcttac cgacctcaat tatacattga gtgtaatcac tctaatgaac aatacattgc
     2881 acacaatact tgaggatcca ggaatggcgt attttcctta tatagcatct gtcttaacag
     2941 ttttgtttgc gctacataaa gcatccattc caacaatgaa aattgcattg aaaacgtcaa
     3001 aatgttcata taaagtggtg aaatattgta ttgtaacaat ttttaatacg ttgttaaaat
     3061 tggcaggtta taaagagcag ataactacta aagatgagat agaaaagcaa atggacagag
     3121 tagtcaaaga aatgagacgc cagctagaaa tgattgacaa attgactaca cgtgaaattg
     3181 aacaagtaga gttgcttaaa cgcatttacg ataaattgac ggtgcaaacg acaggtgaaa
     3241 tagatatgac aaaagagatc aatcaaaaaa acgtgagaac gctagaagaa tgggaaagtg
     3301 gaaaaaatcc ttatgaacca agagaagtga ctgcagcaat gtaagaggtt gagctgccgt
     3361 cgactgtcct cggaagcggc ggagttcttt acagtaagca ccatcggacc tgatggctga
     3421 ctgagaagcc acagtcagcc atatcgcgtg tggctcaagc cttaatcccg tttaaccaat
     3481 ccggtcagca ccggacgtta atggaaggaa cggtcttaat gtgaccgggt cggcatggca
     3541 tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc actcggatgg
     3601 ctaagggaga gctcaaaaaa aaggatccgg ctgctaacaa agcccgaaag gaagctgagt
     3661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3721 tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcctct agagtcgacc
     3781 tgcaggcatg caagcttgta ttctatagtg tcacctaaat cgtat
//
