LOCUS       sequence-176103-        4133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 NSP2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-NSP2SA11
FEATURES             Location/Qualifiers
     source          1..4133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(641..1432,1433..1501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1264..1283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1502..1606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1674..1692
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1730..1752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1852..1871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2081..2100
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2390..2408
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2390..2407
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2476..2523)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2519..2537
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(3741..3759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3844..3861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4015..4133,1..470))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4095..4114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
       61 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      301 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
      841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
      901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
      961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1621 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1681 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1741 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     1861 agcagattgt actgagagtg caccattcga cgctctccct tatgcgactc ctgcattaag
     1921 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg
     1981 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     2041 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     2101 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     2161 tctggctagc gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt
     2221 taccagaaac tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag
     2281 atgatagggt ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt
     2341 tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac
     2401 actatagaat acaagcttgc atgcctgcag gtcgactcta gaggatctcg atccggatat
     2461 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     2521 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     2581 cggatccttt tttttgagct ctcccttagc catccgagtg gacgacgtcc tccttcggat
     2641 gcccaggtcg gaccgcgagg aggtggagat gccatgccga cccggtcaca taagcgcttt
     2701 ctattcttgc taagccatca tcatcctcaa attgatagcg aattaaacgc caacttgaga
     2761 aacttcgtcc atttttctat ccgttgacag ccctttaaat ggattttttt ctggtttcat
     2821 tttttggaaa agcaaccttt tacacacgtc aagtgtatta ccctgtttca ttgatgatgt
     2881 aaatgcatac cagttttgcc aaataattct ttgatcaagt aggttaaaat cattattaac
     2941 tccagtttta acattactct tgaaagttgc atatactctg tcagcgtgat tagcaactga
     3001 tgaatacttt acaattctat aatgaccctt accatgtgtg attacagcaa acttgttata
     3061 ttgccatcga agttcagcga ctaattcttt aacatgaaca tctgaaattg gtttatcttc
     3121 gttcaatgta actttatatt caataaaatt ctgatccaga attggcatca attgatgttc
     3181 taaataagtt agtttccaca tggtgaaggc agcgttttga aatacaattt ctcctccttc
     3241 tgcagttatt gtagtttcaa tttctttaga ctgtccaata gcaatcagtg ttgatttcaa
     3301 aagtaaatct tttgaatgaa atagaatatc ctgtggattt tctttacgta tcaccaaatt
     3361 ttctaaatgc cttattgata ctactcgtga taatacatta gagacgtttg cttgcgtcac
     3421 tttcaatgaa tttagagctt cacatatcaa catagccacc ttagtaaaca taattgtttc
     3481 aaaattcatg cctcttgaat tatcattagt attataccgt ttcttaaatt gaggaggcgg
     3541 tgctattcca taaataattg aatcataaaa cttatccatg tcctttttgt ctactttagc
     3601 tgtcagcata gctttaatag ctaaattatt aaaaggaata aatttatagc tatcattctc
     3661 caaatgagga tagcaaaagc aagctagctc agccatggct acaccgcaag gctcaaacgg
     3721 cgactgagac gctttaaaag cctatagtga gtcgtattaa cccgggatcg atatccccct
     3781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgc
//
