LOCUS       sequence-176123-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..81
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..813
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 tttagtgacg tcagatccgc tagcgccacc atgggcgtgg ccgacttgat caagaagttc
       61 gagtccatct ccaaggagga gggggatcca ccggtcgcca ccatggtgtc taagggcgaa
      121 gagctgatca aggaaaatat gcgtatgaag gtggtcatgg aaggttcggt caacggccac
      181 caattcaaat gcacaggtga aggagaaggc aatccgtaca tgggaactca aaccatgagg
      241 atcaaagtca tcgagggagg acccctgcca tttgcctttg acattcttgc cacgtcgttc
      301 atgtatggca gccgtacttt tatcaagtac ccgaaaggca ttcctgattt ctttaaacag
      361 tcctttcctg agggttttac ttgggaaaga gttacgagat acgaagatgg tggagtcgtc
      421 accgtcatgc aggacaccag ccttgaggat ggctgtctcg tttaccacgt ccaagtcaga
      481 ggggtaaact ttccctccaa tggtcccgtg atgcagaaga agaccaaggg ttgggagcct
      541 aatacagaga tgatgtatcc agcagatggt ggtctgaggg gatacactca tatggcactg
      601 aaagttgatg gtggtggcca tctgtcttgc tctttcgtaa caacttacag gtcaaaaaag
      661 accgtcggga acatcaagat gcccggtatc catgccgttg atcaccgcct ggaaaggtta
      721 gaggaaagtg acaatgaaat gttcgtagta caacgcgaac acgcagttgc caagttcgcc
      781 gggcttggtg gtgggatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
      841 cagccatacc acatttgtag aggttttact tgctttaaaa a
//
