LOCUS       sequence-176125-        7687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor with prostate cancer
            somatic mutation H874Y from CWR22 xenograft.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Tan J, Sharief Y, Hamil KG, Gregory CW, Zang DY, Sar M, Gumerlock 
            PH, deVere White RW, Pretlow TG, Harris SE, Wilson EM, Mohler JL, 
            French FS
  TITLE     Dehydroepiandrosterone activates mutant androgen receptors expressed
            in the androgen-dependent human prostate cancer xenograft CWR22 and 
            LNCaP cells.
  JOURNAL   Mol Endocrinol. 1997 Apr;11(4):450-9. doi: 10.1210/mend.11.4.9906.
  PUBMED    9092797
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1210/mend.11.4";
            journalName: "Mol Endocrinol"; date: "1997-04"; volume: "11"; issue:
            "4"; pages: "450-9"
COMMENT     Sequence Label: pCMV-hAR-H874Y
FEATURES             Location/Qualifiers
     source          1..7687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1510..2132
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1709..1728)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2156..2176)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2161..2490
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2341..2476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2528..2547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2529..2547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2561..2577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2561..2577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2574..2596)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2585..2601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2609..2615,2616..2633,2634..2639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2654..2675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2792..2809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2963..3551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3043..3062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3722..4513,4514..4582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4345..4364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4583..4687)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4714..5169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4801..4820)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5011..5032
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5295..5317
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5309..5326
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5310..5326
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5487..5866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5867..6070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6021..6041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaggcgggag ctgtagcccc ctacggctac actcggcccc ctcaggggct ggcgggccag
       61 gaaagcgact tcaccgcacc tgatgtgtgg taccctggcg gcatggtgag cagagtgccc
      121 tatcccagtc ccacttgtgt caaaagcgaa atgggcccct ggatggatag ctactccgga
      181 ccttacgggg acatgcgttt ggagactgcc agggaccatg ttttgcccat tgactattac
      241 tttccacccc agaagacctg cctgatctgt ggagatgaag cttctgggtg tcactatgga
      301 gctctcacat gtggaagctg caaggtcttc ttcaaaagag ccgctgaagg gaaacagaag
      361 tacctgtgcg ccagcagaaa tgattgcact attgataaat tccgaaggaa aaattgtcca
      421 tcttgtcgtc ttcggaaatg ttatgaagca gggatgactc tgggagcccg gaagctgaag
      481 aaacttggta atctgaaact acaggaggaa ggagaggctt ccagcaccac cagccccact
      541 gaggagacaa cccagaagct gacagtgtca cacattgaag gctatgaatg tcagcccatc
      601 tttctgaatg tcctggaagc cattgagcca ggtgtagtgt gtgctggaca cgacaacaac
      661 cagcccgact cctttgcagc cttgctctct agcctcaatg aactgggaga gagacagctt
      721 gtacacgtgg tcaagtgggc caaggccttg cctggcttcc gcaacttaca cgtggacgac
      781 cagatggctg tcattcagta ctcctggatg gggctcatgg tgtttgccat gggctggcga
      841 tccttcacca atgtcaactc caggatgctc tacttcgccc ctgatctggt tttcaatgag
      901 taccgcatgc acaagtcccg gatgtacagc cagtgtgtcc gaatgaggca cctctctcaa
      961 gagtttggat ggctccaaat caccccccag gaattcctgt gcatgaaagc actgctactc
     1021 ttcagcatta ttccagtgga tgggctgaaa aatcaaaaat tctttgatga acttcgaatg
     1081 aactacatca aggaactcga tcgtatcatt gcatgcaaaa gaaaaaatcc cacatcctgc
     1141 tcaagacgct tctaccagct caccaagctc ctggactccg tgcagcctat tgcgagagag
     1201 ctgtatcagt tcacttttga cctgctaatc aagtcacaca tggtgagcgt ggactttccg
     1261 gaaatgatgg cagagatcat ctctgtgcaa gtgcccaaga tcctttctgg gaaagtcaag
     1321 cccatctatt tccacaccca gtgaagcatt ggaaacccta tttccccacc ccagctcatg
     1381 ccccctttca gatgtcttct gcctgttata actctgcact actcctctgc agtgccttgg
     1441 ggaatttcct ctattgatgt acagtctgtc atgagcttgc atgcctgcag gtcgactcta
     1501 gaggatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     1561 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     1621 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     1681 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     1741 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     1801 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     1861 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     1921 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     1981 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     2041 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     2101 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat
     2161 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2221 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     2281 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2461 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa
     2521 gttaattccc tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt
     2581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4681 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     4741 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4801 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4861 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4921 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4981 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5041 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5101 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5161 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5221 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5281 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctgatcta
     5341 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5401 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5461 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     5521 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5581 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5641 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5701 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     5761 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     5821 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5881 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5941 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6001 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6061 aagcagagct cgtttagtga accgtcagaa ttcagatctg tccaccgtgt gtcttcttct
     6121 gcacgagact ttgaggctgt cagagcgctt tttgcgtggt tgctcccgca agtttccttc
     6181 tctggagctt cccgcaggtg ggcagctagc tgcagcgact accgcatcat cacagcctgt
     6241 tgaactcttc tgagcaagag aaggggaggc ggggtaaggg aagtaggtgg aagattcagc
     6301 caagctcaag gatggaagtg cagttagggc tgggaagggt ctaccctcgg ccgccgtcca
     6361 agacctaccg aggagctttc cagaatctgt tccagagcgt gcgcgaagtg atccagaacc
     6421 cgggccccag gcacccagag gccgcgagcg cagcacctcc cggcgccagt ttgctgctgc
     6481 tgcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     6541 agcagcagca gcagcaagag actagcccca ggcagcagca gcagcagcag ggtgaggatg
     6601 gttctcccca agcccatcgt agaggcccca caggctacct ggtcctggat gaggaacagc
     6661 aaccttcaca gccgcagtcg gccctggagt gccaccccga gagaggttgc gtcccagagc
     6721 ctggagccgc cgtggccgcc agcaaggggc tgccgcagca gctgccagca cctccggacg
     6781 aggatgactc agctgcccca tccacgttgt ccctgctggg ccccactttc cccggcttaa
     6841 gcagctgctc cgctgacctt aaagacatcc tgagcgaggc cagcaccatg caactccttc
     6901 agcaacagca gcaggaagca gtatccgaag gcagcagcag cgggagagcg agggaggcct
     6961 cgggggctcc cacttcctcc aaggacaatt acttaggggg cacttcgacc atttctgaca
     7021 acgccaagga gttgtgtaag gcagtgtcgg tgtccatggg cctgggtgtg gaggcgttgg
     7081 agcatctgag tccaggggaa cagcttcggg gggattgcat gtacgcccca cttttgggag
     7141 ttccacccgc tgtgcgtccc actccttgtg ccccattggc cgaatgcaaa ggttctctgc
     7201 tagacgacag cgcaggcaag agcactgaag atactgctga gtattcccct ttcaagggag
     7261 gttacaccaa agggctagaa ggcgagagcc taggctgctc tggcagcgct gcagcaggga
     7321 gctccgggac acttgaactg ccgtctaccc tgtctctcta caagtccgga gcactggacg
     7381 aggcagctgc gtaccagagt cgcgactact acaactttcc actggctctg gccggaccgc
     7441 cgccccctcc gccgcctccc catccccacg ctcgcatcaa gctggagaac ccgctggact
     7501 acggcagcgc ctgggcggct gcggcggcgc agtgccgcta tggggacctg gcgagcctgc
     7561 atggcgcggg tgcagcggga cccggttctg ggtcaccctc agccgccgct tcctcatcct
     7621 ggcacactct cttcacagcc gaagaaggcc agttgtatgg accgtgtggt ggtggtgggg
     7681 gtggtgg
//
