LOCUS       sequence-176127-        6680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor with deletion of 
            120-472 amino acid residues.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Bai S, Wilson EM
  TITLE     Epidermal-growth-factor-dependent phosphorylation and 
            ubiquitinylation of MAGE-11 regulates its interaction with the 
            androgen receptor.
  JOURNAL   Mol Cell Biol. 2008 Mar;28(6):1947-63. doi: 10.1128/MCB.01672-07. 
            Epub 2008 Jan 22.
  PUBMED    18212060
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01672-07"; journalName: "Mol Cell Biol"; date: 
            "2008-03"; volume: "28"; issue: "6"; pages: "1947-63"
COMMENT     Sequence Label: pCMV-hARdelta120-472
FEATURES             Location/Qualifiers
     source          1..6680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2704..3326
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2903..2922)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3350..3370)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3355..3684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3535..3670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3722..3741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3723..3741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3755..3771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3755..3771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3768..3790)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3779..3795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3803..3809,3810..3827,3828..3833))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3848..3869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3986..4003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4157..4745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4237..4256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4916..5707,5708..5776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5539..5558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5777..5881)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5908..6363
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5995..6014)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6205..6226
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6489..6511
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6503..6520
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6504..6520
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctgtccacc gtgtgtcttc ttctgcacga gactttgagg ctgtcagagc
      661 gctttttgcg tggttgctcc cgcaagtttc cttctctgga gcttcccgca ggtgggcagc
      721 tagctgcagc gactaccgca tcatcacagc ctgttgaact cttctgagca agagaagggg
      781 aggcggggta agggaagtag gtggaagatt cagccaagct caaggatgga agtgcagtta
      841 gggctgggaa gggtctaccc tcggccgccg tccaagacct accgaggagc tttccagaat
      901 ctgttccaga gcgtgcgcga agtgatccag aacccgggcc ccaggcaccc agaggccgcg
      961 agcgcagcac ctcccggcgc cagtttgctg ctgctgcagc agcagcagca gcagcagcag
     1021 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca agagactagc
     1081 cccaggcagc agcagcagca gcagggtgag gatggttctc cccaagccca tcgtagaggc
     1141 cccacaggct acctggtcct ggatgaggaa cagcaacctt cacagccgca gtcggaggcg
     1201 ggagctgtag ccccctacgg ctacactcgg ccccctcagg ggctggcggg ccaggaaagc
     1261 gacttcaccg cacctgatgt gtggtaccct ggcggcatgg tgagcagagt gccctatccc
     1321 agtcccactt gtgtcaaaag cgaaatgggc ccctggatgg atagctactc cggaccttac
     1381 ggggacatgc gtttggagac tgccagggac catgttttgc ccattgacta ttactttcca
     1441 ccccagaaga cctgcctgat ctgtggagat gaagcttctg ggtgtcacta tggagctctc
     1501 acatgtggaa gctgcaaggt cttcttcaaa agagccgctg aagggaaaca gaagtacctg
     1561 tgcgccagca gaaatgattg cactattgat aaattccgaa ggaaaaattg tccatcttgt
     1621 cgtcttcgga aatgttatga agcagggatg actctgggag cccggaagct gaagaaactt
     1681 ggtaatctga aactacagga ggaaggagag gcttccagca ccaccagccc cactgaggag
     1741 acaacccaga agctgacagt gtcacacatt gaaggctatg aatgtcagcc catctttctg
     1801 aatgtcctgg aagccattga gccaggtgta gtgtgtgctg gacacgacaa caaccagccc
     1861 gactcctttg cagccttgct ctctagcctc aatgaactgg gagagagaca gcttgtacac
     1921 gtggtcaagt gggccaaggc cttgcctggc ttccgcaact tacacgtgga cgaccagatg
     1981 gctgtcattc agtactcctg gatggggctc atggtgtttg ccatgggctg gcgatccttc
     2041 accaatgtca actccaggat gctctacttc gcccctgatc tggttttcaa tgagtaccgc
     2101 atgcacaagt cccggatgta cagccagtgt gtccgaatga ggcacctctc tcaagagttt
     2161 ggatggctcc aaatcacccc ccaggaattc ctgtgcatga aagcactgct actcttcagc
     2221 attattccag tggatgggct gaaaaatcaa aaattctttg atgaacttcg aatgaactac
     2281 atcaaggaac tcgatcgtat cattgcatgc aaaagaaaaa atcccacatc ctgctcaaga
     2341 cgcttctacc agctcaccaa gctcctggac tccgtgcagc ctattgcgag agagctgcat
     2401 cagttcactt ttgacctgct aatcaagtca cacatggtga gcgtggactt tccggaaatg
     2461 atggcagaga tcatctctgt gcaagtgccc aagatccttt ctgggaaagt caagcccatc
     2521 tatttccaca cccagtgaag cattggaaac cctatttccc caccccagct catgccccct
     2581 ttcagatgtc ttctgcctgt tataactctg cactactcct ctgcagtgcc ttggggaatt
     2641 tcctctattg atgtacagtc tgtcatgagc ttgcatgcct gcaggtcgac tctagaggat
     2701 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2761 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     2821 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     2881 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     2941 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     3001 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     3061 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     3121 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     3181 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     3241 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     3301 tccactcagt agatgcctgt tgaattgggt acgcggccag cttggctgtg gaatgtgtgt
     3361 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3421 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3481 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3661 tttggaggcc taggcttttg caaaaagctc ctcgaggaac tgaaaaacca gaaagttaat
     3721 tccctatagt gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     3781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5881 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     5941 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6001 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6061 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6121 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6181 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6241 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6301 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     6361 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6421 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6481 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga tctatacatt
     6541 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     6601 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     6661 tccaatatga ccgccatgtt
//
