LOCUS       sequence-176132-        7730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor with mutation of 
            N-terminal FxxLF motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   He B, Kemppainen JA, Wilson EM
  TITLE     FXXLF and WXXLF sequences mediate the NH2-terminal interaction with 
            the ligand binding domain of the androgen receptor.
  JOURNAL   J Biol Chem. 2000 Jul 28;275(30):22986-94.
  PUBMED    10816582
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2000-07-28"; volume: "275(30)"; pages: "22986-94"
COMMENT     Sequence Label: pCMV-hAR-F23A
FEATURES             Location/Qualifiers
     source          1..7730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1509..2131
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1708..1727)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2155..2175)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2160..2489
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2340..2475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2527..2546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2528..2546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2560..2576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2560..2576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2573..2595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2584..2600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2608..2614,2615..2632,2633..2638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2653..2674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2791..2808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2962..3550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3042..3061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3721..4512,4513..4581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4344..4363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4582..4686)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4713..5168
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4800..4819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5010..5031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5294..5316
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5308..5325
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5309..5325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5486..5865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5866..6069
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6020..6040
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aggcgggagc tgtagccccc tacggctaca ctcggccccc tcaggggctg gcgggccagg
       61 aaagcgactt caccgcacct gatgtgtggt accctggcgg catggtgagc agagtgccct
      121 atcccagtcc cacttgtgtc aaaagcgaaa tgggcccctg gatggatagc tactccggac
      181 cttacgggga catgcgtttg gagactgcca gggaccatgt tttgcccatt gactattact
      241 ttccacccca gaagacctgc ctgatctgtg gagatgaagc ttctgggtgt cactatggag
      301 ctctcacatg tggaagctgc aaggtcttct tcaaaagagc cgctgaaggg aaacagaagt
      361 acctgtgcgc cagcagaaat gattgcacta ttgataaatt ccgaaggaaa aattgtccat
      421 cttgtcgtct tcggaaatgt tatgaagcag ggatgactct gggagcccgg aagctgaaga
      481 aacttggtaa tctgaaacta caggaggaag gagaggcttc cagcaccacc agccccactg
      541 aggagacaac ccagaagctg acagtgtcac acattgaagg ctatgaatgt cagcccatct
      601 ttctgaatgt cctggaagcc attgagccag gtgtagtgtg tgctggacac gacaacaacc
      661 agcccgactc ctttgcagcc ttgctctcta gcctcaatga actgggagag agacagcttg
      721 tacacgtggt caagtgggcc aaggccttgc ctggcttccg caacttacac gtggacgacc
      781 agatggctgt cattcagtac tcctggatgg ggctcatggt gtttgccatg ggctggcgat
      841 ccttcaccaa tgtcaactcc aggatgctct acttcgcccc tgatctggtt ttcaatgagt
      901 accgcatgca caagtcccgg atgtacagcc agtgtgtccg aatgaggcac ctctctcaag
      961 agtttggatg gctccaaatc accccccagg aattcctgtg catgaaagca ctgctactct
     1021 tcagcattat tccagtggat gggctgaaaa atcaaaaatt ctttgatgaa cttcgaatga
     1081 actacatcaa ggaactcgat cgtatcattg catgcaaaag aaaaaatccc acatcctgct
     1141 caagacgctt ctaccagctc accaagctcc tggactccgt gcagcctatt gcgagagagc
     1201 tgcatcagtt cacttttgac ctgctaatca agtcacacat ggtgagcgtg gactttccgg
     1261 aaatgatggc agagatcatc tctgtgcaag tgcccaagat cctttctggg aaagtcaagc
     1321 ccatctattt ccacacccag tgaagcattg gaaaccctat ttccccaccc cagctcatgc
     1381 cccctttcag atgtcttctg cctgttataa ctctgcacta ctcctctgca gtgccttggg
     1441 gaatttcctc tattgatgta cagtctgtca tgagcttgca tgcctgcagg tcgactctag
     1501 aggatcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     1561 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     1621 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     1681 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     1741 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     1801 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     1861 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     1921 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     1981 tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag cataggctac
     2041 ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc ctctcatgcg
     2101 ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcttgg ctgtggaatg
     2161 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2221 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2281 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2461 gcttttttgg aggcctaggc ttttgcaaaa agctcctcga ggaactgaaa aaccagaaag
     2521 ttaattccct atagtgagtc gtattaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     2581 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2641 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2701 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2761 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2821 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2881 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2941 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3001 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3061 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3361 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3601 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3661 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3721 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3781 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3841 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3901 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3961 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4021 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4081 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4141 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4201 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4261 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4321 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4381 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4441 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4501 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4561 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4681 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     4741 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4801 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4861 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4921 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4981 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5041 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5101 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5161 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5221 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5281 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgatctat
     5341 acattgaatc aatattggca attagccata ttagtcattg gttatatagc ataaatcaat
     5401 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     5461 tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     5521 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5581 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     5641 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     5701 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     5761 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     5821 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     5881 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     5941 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6001 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6061 agcagagctc gtttagtgaa ccgtcagaat tcagatctgt ccaccgtgtg tcttcttctg
     6121 cacgagactt tgaggctgtc agagcgcttt ttgcgtggtt gctcccgcaa gtttccttct
     6181 ctggagcttc ccgcaggtgg gcagctagct gcagcgacta ccgcatcatc acagcctgtt
     6241 gaactcttct gagcaagaga aggggaggcg gggtaaggga agtaggtgga agattcagcc
     6301 aagctcaagg atggaagtgc agttagggct gggaagggtc taccctcggc cgccgtccaa
     6361 gacctaccga ggagctgccc agaatctgtt ccagagcgtg cgcgaagtga tccagaaccc
     6421 gggccccagg cacccagagg ccgcgagcgc agcacctccc ggcgccagtt tgctgctgct
     6481 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     6541 gcagcagcaa gagactagcc ccaggcagca gcagcagcag cagggtgagg atggttctcc
     6601 ccaagcccat cgtagaggcc ccacaggcta cctggtcctg gatgaggaac agcaaccttc
     6661 acagccgcag tcggccctgg agtgccaccc cgagagaggt tgcgtcccag agcctggagc
     6721 cgccgtggcc gccagcaagg ggctgccgca gcagctgcca gcacctccgg acgaggatga
     6781 ctcagctgcc ccatccacgt tgtccctgct gggccccact ttccccggct taagcagctg
     6841 ctccgctgac cttaaagaca tcctgagcga ggccagcacc atgcaactcc ttcagcaaca
     6901 gcagcaggaa gcagtatccg aaggcagcag cagcgggaga gcgagggagg cctcgggggc
     6961 tcccacttcc tccaaggaca attacttagg gggcacttcg accatttctg acaacgccaa
     7021 ggagttgtgt aaggcagtgt cggtgtccat gggcctgggt gtggaggcgt tggagcatct
     7081 gagtccaggg gaacagcttc ggggggattg catgtacgcc ccacttttgg gagttccacc
     7141 cgctgtgcgt cccactcctt gtgccccatt ggccgaatgc aaaggttctc tgctagacga
     7201 cagcgcaggc aagagcactg aagatactgc tgagtattcc cctttcaagg gaggttacac
     7261 caaagggcta gaaggcgaga gcctaggctg ctctggcagc gctgcagcag ggagctccgg
     7321 gacacttgaa ctgccgtcta ccctgtctct ctacaagtcc ggagcactgg acgaggcagc
     7381 tgcgtaccag agtcgcgact actacaactt tccactggct ctggccggac cgccgccccc
     7441 tccgccgcct ccccatcccc acgctcgcat caagctggag aacccgctgg actacggcag
     7501 cgcctgggcg gctgcggcgg cgcagtgccg ctatggggac ctggcgagcc tgcatggcgc
     7561 gggtgcagcg ggacccggtt ctgggtcacc ctcagccgcc gcttcctcat cctggcacac
     7621 tctcttcaca gccgaagaag gccagttgta tggaccgtgt ggtggtggtg ggggtggtgg
     7681 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg
//
