LOCUS       sequence-176135-        7510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor with deletion of 
            amino acids 538-614, deletion of AR DNA binding domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Zhou ZX, Sar M, Simental JA, Lane MV, Wilson EM
  TITLE     A ligand-dependent bipartite nuclear targeting signal in the human 
            androgen receptor. Requirement for the DNA-binding domain and 
            modulation by NH2-terminal and carboxyl-terminal sequences.
  JOURNAL   J Biol Chem. 1994 May 6;269(18):13115-23.
  PUBMED    8175737
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1994-05-6"; volume: "269(18)"; pages: "13115-23"
COMMENT     Sequence Label: pCMV-hARdelta538-614
FEATURES             Location/Qualifiers
     source          1..7510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1321..1943
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1520..1539)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(1967..1987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1972..2301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2152..2287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2339..2358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2340..2358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2372..2388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2372..2388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2385..2407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2396..2412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2420..2426,2427..2444,2445..2450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2465..2486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2603..2620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2774..3362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2854..2873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3533..4324,4325..4393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4156..4175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4394..4498)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4525..4980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4612..4631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4822..4843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5106..5128
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5120..5137
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5121..5137
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5298..5677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5678..5881
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5832..5852
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcgaggcggg agctgtagcc
       61 ccctacggct acactcggcc ccctcagggg ctggcgggcc aggaaagcga cttcaccgca
      121 cctgatgtgt ggtaccctgg cggcatggtg agcagagtgc cctatcccag tcccacttgt
      181 gtcaaaagcg aaatgggccc ctggatggat agctactccg gaccttacgg ggacatgcgt
      241 cttcggaaat gttatgaagc agggatgact ctgggagccc ggaagctgaa gaaacttggt
      301 aatctgaaac tacaggagga aggagaggct tccagcacca ccagccccac tgaggagaca
      361 acccagaagc tgacagtgtc acacattgaa ggctatgaat gtcagcccat ctttctgaat
      421 gtcctggaag ccattgagcc aggtgtagtg tgtgctggac acgacaacaa ccagcccgac
      481 tcctttgcag ccttgctctc tagcctcaat gaactgggag agagacagct tgtacacgtg
      541 gtcaagtggg ccaaggcctt gcctggcttc cgcaacttac acgtggacga ccagatggct
      601 gtcattcagt actcctggat ggggctcatg gtgtttgcca tgggctggcg atccttcacc
      661 aatgtcaact ccaggatgct ctacttcgcc cctgatctgg ttttcaatga gtaccgcatg
      721 cacaagtccc ggatgtacag ccagtgtgtc cgaatgaggc acctctctca agagtttgga
      781 tggctccaaa tcacccccca ggaattcctg tgcatgaaag cactgctact cttcagcatt
      841 attccagtgg atgggctgaa aaatcaaaaa ttctttgatg aacttcgaat gaactacatc
      901 aaggaactcg atcgtatcat tgcatgcaaa agaaaaaatc ccacatcctg ctcaagacgc
      961 ttctaccagc tcaccaagct cctggactcc gtgcagccta ttgcgagaga gctgcatcag
     1021 ttcacttttg acctgctaat caagtcacac atggtgagcg tggactttcc ggaaatgatg
     1081 gcagagatca tctctgtgca agtgcccaag atcctttctg ggaaagtcaa gcccatctat
     1141 ttccacaccc agtgaagcat tggaaaccct atttccccac cccagctcat gccccctttc
     1201 agatgtcttc tgcctgttat aactctgcac tactcctctg cagtgccttg gggaatttcc
     1261 tctattgatg tacagtctgt catgagcttg catgcctgca ggtcgactct agaggatccc
     1321 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     1381 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     1441 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     1501 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     1561 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     1621 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     1681 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     1741 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     1801 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
     1861 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
     1921 actcagtaga tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag
     1981 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2101 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2221 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2281 ggaggcctag gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc
     2341 ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     2401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2701 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2761 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2941 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3061 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3181 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3301 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4501 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4741 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4861 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4921 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4981 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     5041 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     5101 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
     5161 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
     5221 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     5281 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     5341 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     5401 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     5461 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     5521 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     5581 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     5641 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     5701 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     5761 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     5821 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5881 tcgtttagtg aaccgtcaga attcagatct gtccaccgtg tgtcttcttc tgcacgagac
     5941 tttgaggctg tcagagcgct ttttgcgtgg ttgctcccgc aagtttcctt ctctggagct
     6001 tcccgcaggt gggcagctag ctgcagcgac taccgcatca tcacagcctg ttgaactctt
     6061 ctgagcaaga gaaggggagg cggggtaagg gaagtaggtg gaagattcag ccaagctcaa
     6121 ggatggaagt gcagttaggg ctgggaaggg tctaccctcg gccgccgtcc aagacctacc
     6181 gaggagcttt ccagaatctg ttccagagcg tgcgcgaagt gatccagaac ccgggcccca
     6241 ggcacccaga ggccgcgagc gcagcacctc ccggcgccag tttgctgctg ctgcagcagc
     6301 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     6361 agcagcaaga gactagcccc aggcagcagc agcagcagca gggtgaggat ggttctcccc
     6421 aagcccatcg tagaggcccc acaggctacc tggtcctgga tgaggaacag caaccttcac
     6481 agccgcagtc ggccctggag tgccaccccg agagaggttg cgtcccagag cctggagccg
     6541 ccgtggccgc cagcaagggg ctgccgcagc agctgccagc acctccggac gaggatgact
     6601 cagctgcccc atccacgttg tccctgctgg gccccacttt ccccggctta agcagctgct
     6661 ccgctgacct taaagacatc ctgagcgagg ccagcaccat gcaactcctt cagcaacagc
     6721 agcaggaagc agtatccgaa ggcagcagca gcgggagagc gagggaggcc tcgggggctc
     6781 ccacttcctc caaggacaat tacttagggg gcacttcgac catttctgac aacgccaagg
     6841 agttgtgtaa ggcagtgtcg gtgtccatgg gcctgggtgt ggaggcgttg gagcatctga
     6901 gtccagggga acagcttcgg ggggattgca tgtacgcccc acttttggga gttccacccg
     6961 ctgtgcgtcc cactccttgt gccccattgg ccgaatgcaa aggttctctg ctagacgaca
     7021 gcgcaggcaa gagcactgaa gatactgctg agtattcccc tttcaaggga ggttacacca
     7081 aagggctaga aggcgagagc ctaggctgct ctggcagcgc tgcagcaggg agctccggga
     7141 cacttgaact gccgtctacc ctgtctctct acaagtccgg agcactggac gaggcagctg
     7201 cgtaccagag tcgcgactac tacaactttc cactggctct ggccggaccg ccgccccctc
     7261 cgccgcctcc ccatccccac gctcgcatca agctggagaa cccgctggac tacggcagcg
     7321 cctgggcggc tgcggcggcg cagtgccgct atggggacct ggcgagcctg catggcgcgg
     7381 gtgcagcggg acccggttct gggtcaccct cagccgccgc ttcctcatcc tggcacactc
     7441 tcttcacagc cgaagaaggc cagttgtatg gaccgtgtgg tggtggtggg ggtggtggcg
     7501 gcggcggcgg
//
