LOCUS       sequence-176136-        7680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human androgen receptor with DNA
            binding domain mutation CA576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Zhou ZX, Sar M, Simental JA, Lane MV, Wilson EM
  TITLE     A ligand-dependent bipartite nuclear targeting signal in the human 
            androgen receptor. Requirement for the DNA-binding domain and 
            modulation by NH2-terminal and carboxyl-terminal sequences.
  JOURNAL   J Biol Chem. 1994 May 6;269(18):13115-23.
  PUBMED    8175737
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1994-05-6"; volume: "269(18)"; pages: "13115-23"
COMMENT     Sequence Label: pCMV-hAR-CA576-Z
FEATURES             Location/Qualifiers
     source          1..7680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1508..2130
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1707..1726)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2154..2174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2159..2488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2339..2474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2526..2545)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2527..2545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2559..2575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2559..2575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2572..2594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2583..2599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2607..2613,2614..2631,2632..2637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2652..2673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2790..2807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2961..3549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3041..3060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3720..4511,4512..4580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4343..4362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4581..4685)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4712..5167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4799..4818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5009..5030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5293..5315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5307..5324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5308..5324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5485..5864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5865..6068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6019..6039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggcgggagct gtagccccct acggctacac tcggccccct caggggctgg cgggccagga
       61 aagcgacttc accgcacctg atgtgtggta ccctggcggc atggtgagca gagtgcccta
      121 tcccagtccc acttgtgtca aaagcgaaat gggcccctgg atggatagct actccggacc
      181 ttacggggac atgcgtttgg agactgccag ggaccatgtt ttgcccattg actattactt
      241 tccaccccag aagacctgcc tgatctgtgg agatgaagct tctgggtgtc actatggagc
      301 tctcacagct ggaagctgca aggtcttctt caaaagagcc gctgaaggga aacagaagta
      361 cctgtgcgcc agcagaaatg attgcactat tgataaattc cgaaggaaaa attgtccatc
      421 ttgtcgtctt cggaaatgtt atgaagcagg gatgactctg ggagcccgga agctgaagaa
      481 acttggtaat ctgaaactac aggaggaagg agaggcttcc agcaccacca gccccactga
      541 ggagacaacc cagaagctga cagtgtcaca cattgaaggc tatgaatgtc agcccatctt
      601 tctgaatgtc ctggaagcca ttgagccagg tgtagtgtgt gctggacacg acaacaacca
      661 gcccgactcc tttgcagcct tgctctctag cctcaatgaa ctgggagaga gacagcttgt
      721 acacgtggtc aagtgggcca aggccttgcc tggcttccgc aacttacacg tggacgacca
      781 gatggctgtc attcagtact cctggatggg gctcatggtg tttgccatgg gctggcgatc
      841 cttcaccaat gtcaactcca ggatgctcta cttcgcccct gatctggttt tcaatgagta
      901 ccgcatgcac aagtcccgga tgtacagcca gtgtgtccga atgaggcacc tctctcaaga
      961 gtttggatgg ctccaaatca ccccccagga attcctgtgc atgaaagcac tgctactctt
     1021 cagcattatt ccagtggatg ggctgaaaaa tcaaaaattc tttgatgaac ttcgaatgaa
     1081 ctacatcaag gaactcgatc gtatcattgc atgcaaaaga aaaaatccca catcctgctc
     1141 aagacgcttc taccagctca ccaagctcct ggactccgtg cagcctattg cgagagagct
     1201 gcatcagttc acttttgacc tgctaatcaa gtcacacatg gtgagcgtgg actttccgga
     1261 aatgatggca gagatcatct ctgtgcaagt gcccaagatc ctttctggga aagtcaagcc
     1321 catctatttc cacacccagt gaagcattgg aaaccctatt tccccacccc agctcatgcc
     1381 ccctttcaga tgtcttctgc ctgttataac tctgcactac tcctctgcag tgccttgggg
     1441 aatttcctct attgatgtac agtctgtcat gagcttgcat gcctgcaggt cgactctaga
     1501 ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     1561 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     1621 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     1681 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     1741 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     1801 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     1861 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     1921 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     1981 ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc
     2041 tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt
     2101 tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt
     2161 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2221 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2281 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2341 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2401 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     2461 cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt
     2521 taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt tcctgtgtga
     2581 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2641 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2701 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2761 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3361 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3601 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3661 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3721 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3781 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3841 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3901 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3961 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4021 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4081 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4141 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4201 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4261 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4321 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4381 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4441 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4501 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4561 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4621 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4681 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     4741 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4801 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4861 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4921 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5041 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5161 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5221 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5281 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgatctata
     5341 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     5401 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     5461 catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     5521 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5581 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5641 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5701 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     5761 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     5821 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5881 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5941 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     6001 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     6061 gcagagctcg tttagtgaac cgtcagaatt cagatctgtc caccgtgtgt cttcttctgc
     6121 acgagacttt gaggctgtca gagcgctttt tgcgtggttg ctcccgcaag tttccttctc
     6181 tggagcttcc cgcaggtggg cagctagctg cagcgactac cgcatcatca cagcctgttg
     6241 aactcttctg agcaagagaa ggggaggcgg ggtaagggaa gtaggtggaa gattcagcca
     6301 agctcaagga tggaagtgca gttagggctg ggaagggtct accctcggcc gccgtccaag
     6361 acctaccgag gagctttcca gaatctgttc cagagcgtgc gcgaagtgat ccagaacccg
     6421 ggccccaggc acccagaggc cgcgagcgca gcacctcccg gcgccagttt gctgctgctg
     6481 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     6541 cagcagcagc agcaagagac tagccccagg cagcagcagc agcagcaggg tgaggatggt
     6601 tctccccaag cccatcgtag aggccccaca ggctacctgg tcctggatga ggaacagcaa
     6661 ccttcacagc cgcagtcggc cctggagtgc caccccgaga gaggttgcgt cccagagcct
     6721 ggagccgccg tggccgccag caaggggctg ccgcagcagc tgccagcacc tccggacgag
     6781 gatgactcag ctgccccatc cacgttgtcc ctgctgggcc ccactttccc cggcttaagc
     6841 agctgctccg ctgaccttaa agacatcctg agcgaggcca gcaccatgca actccttcag
     6901 caacagcagc aggaagcagt atccgaaggc agcagcagcg ggagagcgag ggaggcctcg
     6961 ggggctccca cttcctccaa ggacaattac ttagggggca cttcgaccat ttctgacaac
     7021 gccaaggagt tgtgtaaggc agtgtcggtg tccatgggcc tgggtgtgga ggcgttggag
     7081 catctgagtc caggggaaca gcttcggggg gattgcatgt acgccccact tttgggagtt
     7141 ccacccgctg tgcgtcccac tccttgtgcc ccattggccg aatgcaaagg ttctctgcta
     7201 gacgacagcg caggcaagag cactgaagat actgctgagt attccccttt caagggaggt
     7261 tacaccaaag ggctagaagg cgagagccta ggctgctctg gcagcgctgc agcagggagc
     7321 tccgggacac ttgaactgcc gtctaccctg tctctctaca agtccggagc actggacgag
     7381 gcagctgcgt accagagtcg cgactactac aactttccac tggctctggc cggaccgccg
     7441 ccccctccgc cgcctcccca tccccacgct cgcatcaagc tggagaaccc gctggactac
     7501 ggcagcgcct gggcggctgc ggcggcgcag tgccgctatg gggacctggc gagcctgcat
     7561 ggcgcgggtg cagcgggacc cggttctggg tcaccctcag ccgccgcttc ctcatcctgg
     7621 cacactctct tcacagccga agaaggccag ttgtatggac cgtgtggtgg tggtgggggt
//
