LOCUS       sequence-176137-        7523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor with deletion of 
            amino acids 500-558 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7523)
  TITLE     Wilson Lab plasmids
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-hARdelta500-558
FEATURES             Location/Qualifiers
     source          1..7523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1332..1954
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1531..1550)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(1978..1998)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1983..2312
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2163..2298
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2350..2369)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2351..2369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2383..2399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2383..2399)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2396..2418)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2407..2423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2431..2437,2438..2455,2456..2461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2476..2497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2614..2631)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2785..3373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2865..2884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3544..4335,4336..4404))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4167..4186
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4405..4509)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4536..4991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4623..4642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4833..4854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5117..5139
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5131..5148
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5132..5148
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5309..5688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5689..5892
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5843..5863
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aggcgggagc tgtagccccc tacggctaca ctcggccccc tcaggggctg gcgggccagg
       61 aaagcgactt caccgcacct tgcctgatct gtggagatga agcttctggg tgtcactatg
      121 gagctctcac atgtggaagc tgcaaggtct tcttcaaaag agccgctgaa gggaaacaga
      181 agtacctgtg cgccagcaga aatgattgca ctattgataa attccgaagg aaaaattgtc
      241 catcttgtcg tcttcggaaa tgttatgaag cagggatgac tctgggagcc cggaagctga
      301 agaaacttgg taatctgaaa ctacaggagg aaggagaggc ttccagcacc accagcccca
      361 ctgaggagac aacccagaag ctgacagtgt cacacattga aggctatgaa tgtcagccca
      421 tctttctgaa tgtcctggaa gccattgagc caggtgtagt gtgtgctgga cacgacaaca
      481 accagcccga ctcctttgca gccttgctct ctagcctcaa tgaactggga gagagacagc
      541 ttgtacacgt ggtcaagtgg gccaaggcct tgcctggctt ccgcaactta cacgtggacg
      601 accagatggc tgtcattcag tactcctgga tggggctcat ggtgtttgcc atgggctggc
      661 gatccttcac caatgtcaac tccaggatgc tctacttcgc ccctgatctg gttttcaatg
      721 agtaccgcat gcacaagtcc cggatgtaca gccagtgtgt ccgaatgagg cacctctctc
      781 aagagtttgg atggctccaa atcacccccc aggaattcct gtgcatgaaa gcactgctac
      841 tcttcagcat tattccagtg gatgggctga aaaatcaaaa attctttgat gaacttcgaa
      901 tgaactacat caaggaactc gatcgtatca ttgcatgcaa aagaaaaaat cccacatcct
      961 gctcaagacg cttctaccag ctcaccaagc tcctggactc cgtgcagcct attgcgagag
     1021 agctgcatca gttcactttt gacctgctaa tcaagtcaca catggtgagc gtggactttc
     1081 cggaaatgat ggcagagatc atctctgtgc aagtgcccaa gatcctttct gggaaagtca
     1141 agcccatcta tttccacacc cagtgaagca ttggaaaccc tatttcccca ccccagctca
     1201 tgcccccttt cagatgtctt ctgcctgtta taactctgca ctactcctct gcagtgcctt
     1261 ggggaatttc ctctattgat gtacagtctg tcatgagctt gcatgcctgc aggtcgactc
     1321 tagaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     1381 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     1441 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     1501 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     1561 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     1621 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     1681 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     1741 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     1801 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc
     1861 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat
     1921 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga
     1981 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2041 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2101 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2161 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2221 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2281 gaggcttttt tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga
     2341 aagttaattc cctatagtga gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt
     2401 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2461 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2521 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3841 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4141 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4501 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     4561 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4621 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4681 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4741 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4801 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4861 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4921 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4981 gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     5041 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     5101 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgatc
     5161 tatacattga atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc
     5221 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
     5281 ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa
     5341 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     5401 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     5461 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     5521 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
     5581 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
     5641 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     5701 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     5761 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     5821 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     5881 ataagcagag ctcgtttagt gaaccgtcag aattcagatc tgtccaccgt gtgtcttctt
     5941 ctgcacgaga ctttgaggct gtcagagcgc tttttgcgtg gttgctcccg caagtttcct
     6001 tctctggagc ttcccgcagg tgggcagcta gctgcagcga ctaccgcatc atcacagcct
     6061 gttgaactct tctgagcaag agaaggggag gcggggtaag ggaagtaggt ggaagattca
     6121 gccaagctca aggatggaag tgcagttagg gctgggaagg gtctaccctc ggccgccgtc
     6181 caagacctac cgaggagctt tccagaatct gttccagagc gtgcgcgaag tgatccagaa
     6241 cccgggcccc aggcacccag aggccgcgag cgcagcacct cccggcgcca gtttgctgct
     6301 gctgcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     6361 gcagcagcag cagcagcaag agactagccc caggcagcag cagcagcagc agggtgagga
     6421 tggttctccc caagcccatc gtagaggccc cacaggctac ctggtcctgg atgaggaaca
     6481 gcaaccttca cagccgcagt cggccctgga gtgccacccc gagagaggtt gcgtcccaga
     6541 gcctggagcc gccgtggccg ccagcaaggg gctgccgcag cagctgccag cacctccgga
     6601 cgaggatgac tcagctgccc catccacgtt gtccctgctg ggccccactt tccccggctt
     6661 aagcagctgc tccgctgacc ttaaagacat cctgagcgag gccagcacca tgcaactcct
     6721 tcagcaacag cagcaggaag cagtatccga aggcagcagc agcgggagag cgagggaggc
     6781 ctcgggggct cccacttcct ccaaggacaa ttacttaggg ggcacttcga ccatttctga
     6841 caacgccaag gagttgtgta aggcagtgtc ggtgtccatg ggcctgggtg tggaggcgtt
     6901 ggagcatctg agtccagggg aacagcttcg gggggattgc atgtacgccc cacttttggg
     6961 agttccaccc gctgtgcgtc ccactccttg tgccccattg gccgaatgca aaggttctct
     7021 gctagacgac agcgcaggca agagcactga agatactgct gagtattccc ctttcaaggg
     7081 aggttacacc aaagggctag aaggcgagag cctaggctgc tctggcagcg ctgcagcagg
     7141 gagctccggg acacttgaac tgccgtctac cctgtctctc tacaagtccg gagcactgga
     7201 cgaggcagct gcgtaccaga gtcgcgacta ctacaacttt ccactggctc tggccggacc
     7261 gccgccccct ccgccgcctc cccatcccca cgctcgcatc aagctggaga acccgctgga
     7321 ctacggcagc gcctgggcgg ctgcggcggc gcagtgccgc tatggggacc tggcgagcct
     7381 gcatggcgcg ggtgcagcgg gacccggttc tgggtcaccc tcagccgccg cttcctcatc
     7441 ctggcacact ctcttcacag ccgaagaagg ccagttgtat ggaccgtgtg gtggtggtgg
     7501 gggtggtggc ggcggcggcg gcg
//
