LOCUS       sequence-176139-        6753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor amino acid residues 
            1-660, insert can be excised with EcoRI/Xba or BamHI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Langley E, Zhou ZX, Wilson EM
  TITLE     Evidence for an anti-parallel orientation of the ligand-activated 
            human androgen receptor dimer.
  JOURNAL   J Biol Chem. 1995 Dec 15;270(50):29983-90. doi: 
            10.1074/jbc.270.50.29983.
  PUBMED    8530400
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.270.50"; journalName: "J Biol Chem"; date: "1995-12-15-
            15"; volume: "270"; issue: "50"; pages: "29983-90"
COMMENT     Sequence Label: pCMV-hAR-1-660
FEATURES             Location/Qualifiers
     source          1..6753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    582..1204
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(781..800)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(1228..1248)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1233..1562
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1413..1548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1600..1619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1601..1619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1633..1649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1633..1649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1646..1668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1657..1673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1681..1687,1688..1705,1706..1711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1726..1747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1864..1881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2035..2623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2115..2134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2794..3585,3586..3654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3417..3436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3655..3759)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3786..4241
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3873..3892)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4083..4104
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4367..4389
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4381..4398
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4382..4398
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4559..4938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4939..5142
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5093..5113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggcgggagct gtagccccct acggctacac tcggccccct caggggctgg cgggccagga
       61 aagcgacttc accgcacctg atgtgtggta ccctggcggc atggtgagca gagtgcccta
      121 tcccagtccc acttgtgtca aaagcgaaat gggcccctgg atggatagct actccggacc
      181 ttacggggac atgcgtttgg agactgccag ggaccatgtt ttgcccattg actattactt
      241 tccaccccag aagacctgcc tgatctgtgg agatgaagct tctgggtgtc actatggagc
      301 tctcacatgt ggaagctgca aggtcttctt caaaagagcc gctgaaggga aacagaagta
      361 cctgtgcgcc agcagaaatg attgcactat tgataaattc cgaaggaaaa attgtccatc
      421 ttgtcgtctt cggaaatgtt atgaagcagg gatgactctg ggagcccgga agctgaagaa
      481 acttggtaat ctgaaactac aggaggaagg agaggcttcc agcaccacca gccccactga
      541 ggagacaacc cagaagctga cagtcgactc tagaggatcc cgggtggcat ccctgtgacc
      601 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
      661 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
      721 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
      781 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
      841 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
      901 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
      961 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     1021 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
     1081 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
     1141 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
     1201 aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1261 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1381 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1441 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1501 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1561 aaaagctcct cgaggaactg aaaaaccaga aagttaattc cctatagtga gtcgtattaa
     1621 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1681 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1741 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1801 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1861 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1921 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2221 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2461 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3781 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4201 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     4261 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     4321 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     4381 tgtaaaacga cggccagtgc caagctgatc tatacattga atcaatattg gcaattagcc
     4441 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     4501 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga
     4561 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4621 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4681 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4741 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4801 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4861 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     4921 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg
     4981 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5041 caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg
     5101 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     5161 aattcagatc tgtccaccgt gtgtcttctt ctgcacgaga ctttgaggct gtcagagcgc
     5221 tttttgcgtg gttgctcccg caagtttcct tctctggagc ttcccgcagg tgggcagcta
     5281 gctgcagcga ctaccgcatc atcacagcct gttgaactct tctgagcaag agaaggggag
     5341 gcggggtaag ggaagtaggt ggaagattca gccaagctca aggatggaag tgcagttagg
     5401 gctgggaagg gtctaccctc ggccgccgtc caagacctac cgaggagctt tccagaatct
     5461 gttccagagc gtgcgcgaag tgatccagaa cccgggcccc aggcacccag aggccgcgag
     5521 cgcagcacct cccggcgcca gtttgctgct gctgcagcag cagcagcagc agcagcagca
     5581 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcaag agactagccc
     5641 caggcagcag cagcagcagc agggtgagga tggttctccc caagcccatc gtagaggccc
     5701 cacaggctac ctggtcctgg atgaggaaca gcaaccttca cagccgcagt cggccctgga
     5761 gtgccacccc gagagaggtt gcgtcccaga gcctggagcc gccgtggccg ccagcaaggg
     5821 gctgccgcag cagctgccag cacctccgga cgaggatgac tcagctgccc catccacgtt
     5881 gtccctgctg ggccccactt tccccggctt aagcagctgc tccgctgacc ttaaagacat
     5941 cctgagcgag gccagcacca tgcaactcct tcagcaacag cagcaggaag cagtatccga
     6001 aggcagcagc agcgggagag cgagggaggc ctcgggggct cccacttcct ccaaggacaa
     6061 ttacttaggg ggcacttcga ccatttctga caacgccaag gagttgtgta aggcagtgtc
     6121 ggtgtccatg ggcctgggtg tggaggcgtt ggagcatctg agtccagggg aacagcttcg
     6181 gggggattgc atgtacgccc cacttttggg agttccaccc gctgtgcgtc ccactccttg
     6241 tgccccattg gccgaatgca aaggttctct gctagacgac agcgcaggca agagcactga
     6301 agatactgct gagtattccc ctttcaaggg aggttacacc aaagggctag aaggcgagag
     6361 cctaggctgc tctggcagcg ctgcagcagg gagctccggg acacttgaac tgccgtctac
     6421 cctgtctctc tacaagtccg gagcactgga cgaggcagct gcgtaccaga gtcgcgacta
     6481 ctacaacttt ccactggctc tggccggacc gccgccccct ccgccgcctc cccatcccca
     6541 cgctcgcatc aagctggaga acccgctgga ctacggcagc gcctgggcgg ctgcggcggc
     6601 gcagtgccgc tatggggacc tggcgagcct gcatggcgcg ggtgcagcgg gacccggttc
     6661 tgggtcaccc tcagccgccg cttcctcatc ctggcacact ctcttcacag ccgaagaagg
     6721 ccagttgtat ggaccgtgtg gtggtggtgg ggg
//
