LOCUS       sequence-176156-        4260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human androgen receptor ligand binding 
            domain 658-919 amino acids linked to GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Askew EB, Gampe RT Jr, Stanley TB, Faggart JL, Wilson EM
  TITLE     Modulation of androgen receptor activation function 2 by 
            testosterone and dihydrotestosterone.
  JOURNAL   J Biol Chem. 2007 Aug 31;282(35):25801-16. doi: 
            10.1074/jbc.M703268200. Epub 2007 Jun 25.
  PUBMED    17591767
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M703268200"; journalName: "J Biol Chem"; date: 
            "2007-08-31- 31"; volume: "282"; issue: "35"; pages: "25801-16"
COMMENT     Sequence Label: GAL-hAR-658-919
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    1884..2018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1921..1940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1975..1994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2081..2100)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2365..2382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2536..3124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2616..2635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3295..4086,4087..4155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3918..3937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4156..4260)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtgtcacac
      841 attgaaggct atgaatgtca gcccatcttt ctgaatgtcc tggaagccat tgagccaggt
      901 gtagtgtgtg ctggacacga caacaaccag cccgactcct ttgcagcctt gctctctagc
      961 ctcaatgaac tgggagagag acagcttgta cacgtggtca agtgggccaa ggccttgcct
     1021 ggcttccgca acttacacgt ggacgaccag atggctgtca ttcagtactc ctggatgggg
     1081 ctcatggtgt ttgccatggg ctggcgatcc ttcaccaatg tcaactccag gatgctctac
     1141 ttcgcccctg atctggtttt caatgagtac cgcatgcaca agtcccggat gtacagccag
     1201 tgtgtccgaa tgaggcacct ctctcaagag tttggatggc tccaaatcac cccccaggaa
     1261 ttcctgtgca tgaaagcact gctactcttc agcattattc cagtggatgg gctgaaaaat
     1321 caaaaattct ttgatgaact tcgaatgaac tacatcaagg aactcgatcg tatcattgca
     1381 tgcaaaagaa aaaatcccac atcctgctca agacgcttct accagctcac caagctcctg
     1441 gactccgtgc agcctattgc gagagagctg catcagttca cttttgacct gctaatcaag
     1501 tcacacatgg tgagcgtgga ctttccggaa atgatggcag agatcatctc tgtgcaagtg
     1561 cccaagatcc tttctgggaa agtcaagccc atctatttcc acacccagtg aagcattgga
     1621 aaccctattt ccccacccca gctcatgccc cctttcagat gtcttctgcc tgttataact
     1681 ctgcactact cctctgcagt gccttgggga atttcctcta ttgatgtaca gtctgtcatg
     1741 agcttgcatg cctgcaggtc gactctagat aagtaatgat cataatcagc catatcacat
     1801 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1861 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1981 tgtccaaact catcaatgta tcttatcatg tctggatcga tcctctacgc cggacgcatc
     2041 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     2101 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     2161 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     2221 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     2281 cataagggag agcgtcgata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
//
