LOCUS       sequence-176170-        6579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of FLAG-tagged human androgen receptor amino 
            acid residues 1-660 in pCMV5-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6579)
  TITLE     Wilson Lab plasmids
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLAG-AR1-660
FEATURES             Location/Qualifiers
     source          1..6579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    575..1197
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(774..793)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(1221..1241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1226..1555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1406..1541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1593..1612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1594..1612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1626..1642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1626..1642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1639..1661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1650..1666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1674..1680,1681..1698,1699..1704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1719..1740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1857..1874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2028..2616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2108..2127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2787..3578,3579..3647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3410..3429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3648..3752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3779..4234
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3866..3885)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4076..4097
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4360..4382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4374..4391
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4375..4391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4552..4931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4932..5135
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5086..5106
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             5182..5205
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcgggagct gtagccccct acggctacac tcggccccct caggggctgg cgggccagga
       61 aagcgacttc accgcacctg atgtgtggta ccctggcggc atggtgagca gagtgcccta
      121 tcccagtccc acttgtgtca aaagcgaaat gggcccctgg atggatagct actccggacc
      181 ttacggggac atgcgtttgg agactgccag ggaccatgtt ttgcccattg actattactt
      241 tccaccccag aagacctgcc tgatctgtgg agatgaagct tctgggtgtc actatggagc
      301 tctcacatgt ggaagctgca aggtcttctt caaaagagcc gctgaaggga aacagaagta
      361 cctgtgcgcc agcagaaatg attgcactat tgataaattc cgaaggaaaa attgtccatc
      421 ttgtcgtctt cggaaatgtt atgaagcagg gatgactctg ggagcccgga agctgaagaa
      481 acttggtaat ctgaaactac aggaggaagg agaggcttcc agcaccacca gccccactga
      541 ggagacaacc cagaagctga cactagagga tcccgggtgg catccctgtg acccctcccc
      601 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
      661 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
      721 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
      781 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
      841 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
      901 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
      961 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     1021 ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat accagcagga
     1081 ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg
     1141 cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg
     1201 tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     1261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     1321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     1381 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     1441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     1501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     1561 cctcgaggaa ctgaaaaacc agaaagttaa ttccctatag tgagtcgtat taaattcgta
     1621 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1681 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2701 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2761 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2821 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2881 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2941 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3001 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3061 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3121 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3181 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3241 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3301 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3361 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3421 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3481 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3541 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3601 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3661 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3781 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3841 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3901 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3961 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4021 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4081 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4141 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4201 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     4261 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     4321 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     4381 cgacggccag tgccaagctg atctatacat tgaatcaata ttggcaatta gccatattag
     4441 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     4501 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat
     4561 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4621 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4681 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4741 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4801 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4861 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     4921 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     4981 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     5041 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     5101 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattcag
     5161 atctagccaa gctcaaggat ggactacaaa gacgatgacg ataaagaagt gcagttaggg
     5221 ctgggaaggg tctaccctcg gccgccgtcc aagacctacc gaggagcttt ccagaatctg
     5281 ttccagagcg tgcgcgaagt gatccagaac ccgggcccca ggcacccaga ggccgcgagc
     5341 gcagcacctc ccggcgccag tttgctgctg ctgcagcagc agcagcagca gcagcagcag
     5401 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcaaga gactagcccc
     5461 aggcagcagc agcagcagca gggtgaggat ggttctcccc aagcccatcg tagaggcccc
     5521 acaggctacc tggtcctgga tgaggaacag caaccttcac agccgcagtc ggccctggag
     5581 tgccaccccg agagaggttg cgtcccagag cctggagccg ccgtggccgc cagcaagggg
     5641 ctgccgcagc agctgccagc acctccggac gaggatgact cagctgcccc atccacgttg
     5701 tccctgctgg gccccacttt ccccggctta agcagctgct ccgctgacct taaagacatc
     5761 ctgagcgagg ccagcaccat gcaactcctt cagcaacagc agcaggaagc agtatccgaa
     5821 ggcagcagca gcgggagagc gagggaggcc tcgggggctc ccacttcctc caaggacaat
     5881 tacttagggg gcacttcgac catttctgac aacgccaagg agttgtgtaa ggcagtgtcg
     5941 gtgtccatgg gcctgggtgt ggaggcgttg gagcatctga gtccagggga acagcttcgg
     6001 ggggattgca tgtacgcccc acttttggga gttccacccg ctgtgcgtcc cactccttgt
     6061 gccccattgg ccgaatgcaa aggttctctg ctagacgaca gcgcaggcaa gagcactgaa
     6121 gatactgctg agtattcccc tttcaaggga ggttacacca aagggctaga aggcgagagc
     6181 ctaggctgct ctggcagcgc tgcagcaggg agctccggga cacttgaact gccgtctacc
     6241 ctgtctctct acaagtccgg agcactggac gaggcagctg cgtaccagag tcgcgactac
     6301 tacaactttc cactggctct ggccggaccg ccgccccctc cgccgcctcc ccatccccac
     6361 gctcgcatca agctggagaa cccgctggac tacggcagcg cctgggcggc tgcggcggcg
     6421 cagtgccgct atggggacct ggcgagcctg catggcgcgg gtgcagcggg acccggttct
     6481 gggtcaccct cagccgccgc ttcctcatcc tggcacactc tcttcacagc cgaagaaggc
     6541 cagttgtatg gaccgtgtgg tggtggtggg ggtggtggc
//
