LOCUS       sequence-176173-        6816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human androgen receptor in pSG5,
            internal AR EcoRI site taken out.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Zhou ZX, He B, Hall SH, Wilson EM, French FS
  TITLE     Domain interactions between coregulator ARA(70) and the androgen 
            receptor (AR).
  JOURNAL   Mol Endocrinol. 2002 Feb;16(2):287-300. doi: 10.1210/mend.16.2.0765.
  PUBMED    11818501
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1210/mend.16.2";
            journalName: "Mol Endocrinol"; date: "2002-02"; volume: "16"; issue:
            "2"; pages: "287-300"
COMMENT     Sequence Label: pSG5-hAR(16)
FEATURES             Location/Qualifiers
     source          1..6816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1373..1494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1410..1429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1464..1483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1528..1545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1699..2287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1779..1798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2458..3249,3250..3318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3081..3100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3319..3423)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3491..3509
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3547..3569)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3669..3688
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3755..4210)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3892..3913)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4104..4123
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4336..4358
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4350..4367
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4351..4367
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4404..4424)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4512..4722
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4534..4730
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4581..4716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4761..5333
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5341..5360
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5388..5407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5388..5406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aggcgggagc tgtagccccc tacggctaca ctcggccccc tcaggggctg gcgggccagg
       61 aaagcgactt caccgcacct gatgtgtggt accctggcgg catggtgagc agagtgccct
      121 atcccagtcc cacttgtgtc aaaagcgaaa tgggcccctg gatggatagc tactccggac
      181 cttacgggga catgcgtttg gagactgcca gggaccatgt tttgcccatt gactattact
      241 ttccacccca gaagacctgc ctgatctgtg gagatgaagc ttctgggtgt cactatggag
      301 ctctcacatg tggaagctgc aaggtcttct tcaaaagagc cgctgaaggg aaacagaagt
      361 acctgtgcgc cagcagaaat gattgcacta ttgataaatt ccgaaggaaa aattgtccat
      421 cttgtcgtct tcggaaatgt tatgaagcag ggatgactct gggagcccgg aagctgaaga
      481 aacttggtaa tctgaaacta caggaggaag gagaggcttc cagcaccacc agccccactg
      541 aggagacaac ccagaagctg acagtgtcac acattgaagg ctatgaatgt cagcccatct
      601 ttctgaatgt cctggaagcc attgagccag gtgtagtgtg tgctggacac gacaacaacc
      661 agcccgactc ctttgcagcc ttgctctcta gcctcaatga actgggagag agacagcttg
      721 tacacgtggt caagtgggcc aaggccttgc ctggcttccg caacttacac gtggacgacc
      781 agatggctgt cattcagtac tcctggatgg ggctcatggt gtttgccatg ggctggcgat
      841 ccttcaccaa tgtcaactcc aggatgctct acttcgcccc tgatctggtt ttcaatgagt
      901 accgcatgca caagtcccgg atgtacagcc agtgtgtccg aatgaggcac ctctctcaag
      961 agtttggatg gctccaaatc accccccagg agttcctgtg catgaaagca ctgctactct
     1021 tcagcattat tccagtggat gggctgaaaa atcaaaaatt ctttgatgaa cttcgaatga
     1081 actacatcaa ggaactcgat cgtatcattg catgcaaaag aaaaaatccc acatcctgct
     1141 caagacgctt ctaccagctc accaagctcc tggactccgt gcagcctatt gcgagagagc
     1201 tgcatcagtt cacttttgac ctgctaatca agtcacacat ggtgagcgtg gactttccgg
     1261 aaatgatggc agagatcatc tctgtgcaag tgcccaagat cctttctggg aaagtcaagc
     1321 ccatctattt ccacacacag tgagaattcg gatccagatc ttattaaagc agaacttgtt
     1381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1501 ctggtcgact ctagactctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3421 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3481 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     3541 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3601 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     3661 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     3721 gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta
     3781 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3841 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3901 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3961 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4021 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4081 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4141 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4201 cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4261 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4321 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtcgactt
     4381 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4441 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ggaaagtccc
     4501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4681 tattccagaa gtagtgaaga ggcttttttg gaggcctagg cttttgcaaa aagctccgga
     4741 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     4801 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     4861 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     4921 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     4981 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     5041 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     5101 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     5161 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     5221 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     5281 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     5341 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac
     5401 tatagggcga attcatggaa gtgcagttag ggctgggaag ggtctaccct cggccgccgt
     5461 ccaagaccta ccgaggagct ttccagaatc tgttccagag cgtgcgcgaa gtgatccaga
     5521 acccgggccc caggcaccca gaggccgcga gcgcagcacc tcccggcgcc agtttgctgc
     5581 tgctgcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     5641 agcagcagca gcagcagcaa gagactagcc ccaggcagca gcagcagcag cagggtgagg
     5701 atggttctcc ccaagcccat cgtagaggcc ccacaggcta cctggtcctg gatgaggaac
     5761 agcaaccttc acagccgcag tcggccctgg agtgccaccc cgagagaggt tgcgtcccag
     5821 agcctggagc cgccgtggcc gccagcaagg ggctgccgca gcagctgcca gcacctccgg
     5881 acgaggatga ctcagctgcc ccatccacgt tgtccctgct gggccccact ttccccggct
     5941 taagcagctg ctccgctgac cttaaagaca tcctgagcga ggccagcacc atgcaactcc
     6001 ttcagcaaca gcagcaggaa gcagtatccg aaggcagcag cagcgggaga gcgagggagg
     6061 cctcgggggc tcccacttcc tccaaggaca attacttagg gggcacttcg accatttctg
     6121 acaacgccaa ggagttgtgt aaggcagtgt cggtgtccat gggcctgggt gtggaggcgt
     6181 tggagcatct gagtccaggg gaacagcttc ggggggattg catgtacgcc ccacttttgg
     6241 gagttccacc cgctgtgcgt cccactcctt gtgccccatt ggccgaatgc aaaggttctc
     6301 tgctagacga cagcgcaggc aagagcactg aagatactgc tgagtattcc cctttcaagg
     6361 gaggttacac caaagggcta gaaggcgaga gcctaggctg ctctggcagc gctgcagcag
     6421 ggagctccgg gacacttgaa ctgccgtcta ccctgtctct ctacaagtcc ggagcactgg
     6481 acgaggcagc tgcgtaccag agtcgcgact actacaactt tccactggct ctggccggac
     6541 cgccgccccc tccgccgcct ccccatcccc acgctcgcat caagctggag aacccgctgg
     6601 actacggcag cgcctgggcg gctgcggcgg cgcagtgccg ctatggggac ctggcgagcc
     6661 tgcatggcgc gggtgcagcg ggacccggtt ctgggtcacc ctcagccgcc gcttcctcat
     6721 cctggcacac tctcttcaca gccgaagaag gccagttgta tggaccgtgt ggtggtggtg
     6781 ggggtggtgg cggcggcggc ggcggcggcg gcggcg
//
