LOCUS       sequence-176177-        6301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal FLAG-tagged full-length human androgen receptor in 
            pIRESneo for stable expression, selection using G418 neomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6301)
  TITLE     Wilson Lab plasmids
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRESneoFLAGhAR
FEATURES             Location/Qualifiers
     source          1..6301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    99..561
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(155..172)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     382..401
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             597..1400
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(660..679)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1270..1289
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1432..1449)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1438..1662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1709..1728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1788..1804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1788..1804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1801..1823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1812..1828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1836..1842,1843..1860,1861..1866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1881..1902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2019..2036)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2190..2778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2270..2289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2949..3740,3741..3809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3572..3591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3810..3914)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3989..4008)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4180..4559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4560..4763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4714..4734
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4808..4827
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4808..4826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4911..4934
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
       61 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
      121 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
      181 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
      241 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
      301 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
      361 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
      421 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
      481 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      541 ctttgaaaaa cacgatgata agcttgccac aacccgggat aattcctgca gccaatatgg
      601 gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      661 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      721 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
      781 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
      841 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
      901 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
      961 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     1021 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     1081 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     1141 ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     1201 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     1261 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     1321 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     1381 ttcttgacga gttcttctga ggggatcaat tctctagagc tcgctgatca gcctcgactg
     1441 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1501 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1561 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     1621 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     1681 ccagctgggg ctcgagtgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1741 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     1801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1981 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2041 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2101 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2641 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2701 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2761 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2941 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3001 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3061 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3121 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3181 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3241 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3301 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3361 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3421 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3481 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3541 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3601 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3661 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3721 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3781 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3901 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     3961 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4021 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     4081 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     4141 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     4201 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4261 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     4321 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     4381 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     4441 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     4501 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     4561 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     4621 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4681 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4741 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     4801 tcgaaattaa tacgactcac tatagggaga cccaagcttg gtaccgagct cggatcgata
     4861 tctgcggccg cgtcgacgga attcagtgga tctagccaag ctcaaggatg gactacaaag
     4921 acgatgacga taaagaagtg cagttagggc tgggaagggt ctaccctcgg ccgccgtcca
     4981 agacctaccg aggagctttc cagaatctgt tccagagcgt gcgcgaagtg atccagaacc
     5041 cgggccccag gcacccagag gccgcgagcg cagcacctcc cggcgccagt ttgctgctgc
     5101 tgcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     5161 agcagcagca gcagcaagag actagcccca ggcagcagca gcagcagcag ggtgaggatg
     5221 gttctcccca agcccatcgt agaggcccca caggctacct ggtcctggat gaggaacagc
     5281 aaccttcaca gccgcagtcg gccctggagt gccaccccga gagaggttgc gtcccagagc
     5341 ctggagccgc cgtggccgcc agcaaggggc tgccgcagca gctgccagca cctccggacg
     5401 aggatgactc agctgcccca tccacgttgt ccctgctggg ccccactttc cccggcttaa
     5461 gcagctgctc cgctgacctt aaagacatcc tgagcgaggc cagcaccatg caactccttc
     5521 agcaacagca gcaggaagca gtatccgaag gcagcagcag cgggagagcg agggaggcct
     5581 cgggggctcc cacttcctcc aaggacaatt acttaggggg cacttcgacc atttctgaca
     5641 acgccaagga gttgtgtaag gcagtgtcgg tgtccatggg cctgggtgtg gaggcgttgg
     5701 agcatctgag tccaggggaa cagcttcggg gggattgcat gtacgcccca cttttgggag
     5761 ttccacccgc tgtgcgtccc actccttgtg ccccattggc cgaatgcaaa ggttctctgc
     5821 tagacgacag cgcaggcaag agcactgaag atactgctga gtattcccct ttcaagggag
     5881 gttacaccaa agggctagaa ggcgagagcc taggctgctc tggcagcgct gcagcaggga
     5941 gctccgggac acttgaactg ccgtctaccc tgtctctcta caagtccgga gcactggacg
     6001 aggcagctgc gtaccagagt cgcgactact acaactttcc actggctctg gccggaccgc
     6061 cgccccctcc gccgcctccc catccccacg ctcgcatcaa gctggagaac ccgctggact
     6121 acggcagcgc ctgggcggct gcggcggcgc agtgccgcta tggggacctg gcgagcctgc
     6181 atggcgcggg tgcagcggga cccggttctg ggtcaccctc agccgccgct tcctcatcct
     6241 ggcacactct cttcacagcc gaagaaggcc agttgtatgg accgtgtggt ggtggtgggg
     6301 g
//
