LOCUS       sequence-176262-        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence 
            GGG for posttranslational coupling to a C-Terminal LPETGG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ott W, Jobst MA, Bauer MS, Durner E, Milles LF, Nash MA, Gaub HE
  TITLE     Elastin-like Polypeptide Linkers for Single-Molecule Force 
            Spectroscopy.
  JOURNAL   ACS Nano. 2017 Jun 12. doi: 10.1021/acsnano.7b02694.
  PUBMED    28591514
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2017 Jun 12. doi: 10.1021/acsnano.7b02694."
COMMENT     Sequence Label: pET28a-GGG-HIS-CBM-Xmod-DocIII
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggggggcg gtcaccacca ccaccaccac ggaatggcca atacaccggt atcaggcaat
       61 ttgaaggttg aattctacaa cagcaatcct tcagatacta ctaactcaat caatcctcag
      121 ttcaaggtta ctaataccgg aagcagtgca attgatttgt ccaaactcac attgagatat
      181 tattatacag tagacggtca gaaagatcag accttctgga gtgaccatgc tgcaataatc
      241 ggcagtaacg gcagctacaa cggaattact tcaaatgtaa aaggaacatt tgtaaaaatg
      301 agttcctcaa caaataacgc agacacctac cttgaaataa gctttacagg cggaactctt
      361 gaaccgggtg cacatgttca gatacaaggt agatttgcaa agaatgactg gagtaactat
      421 acacagtcaa atgactactc attcaagtct gcttcacagt ttgttgaatg ggatcaggta
      481 acagcatact tgaacggtgt tcttgtatgg ggtaaagaac ccggtggcag tgtagtacca
      541 tcaacacagc ctgtaacaac accacctgca acaacaaaac cacctgcaac aacaataccg
      601 ccgtcagatg atccgaatgc cgtggtacca aacactgtta catcagctgt aaagacacag
      661 tatgttgaga tcgaatctgt tgacggcttc tacttcaaca cagaagacaa gttcgataca
      721 gctcagatca agaaggctgt tctccacaca gtttataacg aaggctacac aggtgatgac
      781 ggcgtggctg tagttctcag agaatatgag agcgagcctg ttgatatcac agctgagtta
      841 acattcggtg atgcaacacc tgctaacaca tacaaggctg ttgaaaacaa gttcgattac
      901 gagatccctg tttactacaa caacgctaca ctcaaggatg ctgagggcaa cgatgctaca
      961 gtaactgttt acatcggtct caagggcgat acagacctta acaacatcgt tgacggtcgt
     1021 gacgctacag caacacttac ttactacgct gcaacatcta cagacggcaa ggatgctaca
     1081 acagttgcac tttcaccaag cacactggtt ggcggcaacc ctgagtcagt atatgatgac
     1141 ttctctgcat tccttagcga tgttaaggta gacgctggta aggaactcac aagattcgct
     1201 aagaaggctg agagacttat cgacggtaga gatgcttcaa gcatactcac attctacact
     1261 aagtcttctg tagatcagta caaggatatg gctgctaacg agccaaacaa gctttgggat
     1321 atcgttacag gcgatgctga agaagaataa
//
