LOCUS       sequence-176263-        5001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Creasy CL, Ambrose DM, Chernoff J
  TITLE     The Ste20-like protein kinase, Mst1, dimerizes and contains an 
            inhibitory domain.
  JOURNAL   J Biol Chem. 1996 Aug 30. 271(35):21049-53.
  PUBMED    8702870
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1996 Aug 30. 271(35):21049-53."
COMMENT     Sequence Label: pJ3H-Mst1
FEATURES             Location/Qualifiers
     source          1..5001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..27
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          1647..1712
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1842..1862
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2287..2421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2324..2343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2378..2397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2452..2556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2557..2625,2626..3417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2775..2794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3588..4176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4077..4096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4330..4347
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4362..4502
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4428..4447)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4588..4610
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4648..4668)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4653..4982
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4833..4968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tacccatacg atgttccaga ttacgcttct agaggatccg ccatggagac ggtacagctg
       61 aggaacccgc cgcgccggca gctgaaaaag ttggatgaag atagtttaac caaacaacca
      121 gaagaagtat ttgatgtctt agagaaactt ggagaagggt cctatggcag cgtatacaaa
      181 gctattcata aagagaccgg ccagattgtt gctattaagc aagttcctgt ggaatcagac
      241 ctccaggaga taatcaaaga aatctctata atgcagcaat gtgacagccc tcatgtagtc
      301 aaatattatg gcagttattt taagaacaca gacttatgga tcgttatgga gtactgtggg
      361 gctggttctg tatctgatat cattcgatta cgaaataaaa cgttaacaga agatgaaata
      421 gctacaatat tacaatcaac tcttaaggga cttgaatacc ttcattttat gagaaaaata
      481 caccgagata tcaaggcagg aaatattttg ctaaatacag aaggacatgc aaaacttgca
      541 gattttgggg tagcaggtca acttacagat accatggcca agcggaatac agtgatagga
      601 acaccatttt ggatggctcc agaagtgatt caggaaattg gatacaactg tgtagcagac
      661 atctggtccc tgggaataac tgccatagaa atggctgaag gaaagccccc ttatgctgat
      721 atccatccaa tgagggcaat cttcatgatt cctacaaatc ctcctcccac attccgaaaa
      781 ccagagctat ggtcagataa ctttacagat tttgtgaaac agtgtcttgt aaagagccct
      841 gagcagaggg ccacagccac tcagctcctg cagcacccat ttgtcaggag tgccaaagga
      901 gtgtcaatac tgcgagactt aattaatgaa gccatggatg tgaaactgaa acgccaggaa
      961 tcccagcagc gggaagtgga ccaggacgat gaagaaaact cagaagagga tgaaatggat
     1021 tctggcacga tggttcgagc agtgggtgat gagatgggca ctgtccgagt agccagcacc
     1081 atgactgatg gagccaatac tatgattgag cacgatgaca cgttgccatc acaactgggc
     1141 accatggtga tcaatgcaga ggatgaggaa gaggaaggaa ctatgaaaag aagggatgag
     1201 accatgcagc ctgcgaaacc atcctttctt gaatattttg aacaaaaaga aaaggaaaac
     1261 cagatcaaca gctttggcaa gagtgtacct ggtccactga aaaattcttc agattggaaa
     1321 ataccacagg atggagacta cgagtttctt aagagttgga cagtggagga ccttcagaag
     1381 aggctcttgg ccctggaccc catgatggag caggagattg aagagatccg gcagaagtac
     1441 cagtccaagc ggcagcccat cctggatgcc atagaggcta agaagagacg gcaacaaaac
     1501 ttctgagcaa ggccaggctg tgagggcccc agctccaccc aggctttggg tgaattcatc
     1561 gatggtacca gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     1621 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     1681 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     1741 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     1801 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     1861 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     1921 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     1981 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     2041 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     2101 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     2161 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     2221 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2281 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2401 gtatcttatc atgtctggat ctctgcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2461 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2521 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2581 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2641 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2701 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2761 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2821 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2881 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2941 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3001 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3061 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3121 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3181 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3241 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3301 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3361 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3421 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3481 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3541 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3601 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3661 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3721 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3781 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3841 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3901 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3961 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4021 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4081 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4141 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4201 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4261 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4321 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     4381 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     4441 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     4501 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4561 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4621 catcaccgaa acgcgcgagg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4801 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4861 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4921 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4981 aaagcttcga tcgtcgacat g
//
