LOCUS       sequence-176265-        1290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CohIII-CBM handle with C-Terminal Sortase Recognition Sequence 
            LPETGG for Posttranslational Coupling to a N-terminal GGG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Ott W, Jobst MA, Bauer MS, Durner E, Milles LF, Nash MA, Gaub HE
  TITLE     Elastin-like Polypeptide Linkers for Single-Molecule Force 
            Spectroscopy.
  JOURNAL   ACS Nano. 2017 Jun 12. doi: 10.1021/acsnano.7b02694.
  PUBMED    28591514
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2017 Jun 12. doi: 10.1021/acsnano.7b02694."
COMMENT     Sequence Label: pET28a-CohIII-CBM-HIS-LPETGG
FEATURES             Location/Qualifiers
     source          1..1290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1222..1239
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1246..1269
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atggcgctca cagacagagg aatgacttac gatctcgatc ctaaggatgg atcatctgct
       61 gctacaaagc cagttctcga agtaactaag aaggttttcg acacagctgc tgatgctgct
      121 ggccagactg ttacagttga attcaaggtt agcggtgcag aaggcaagta cgctacaact
      181 ggttatcaca tctactggga tgagagactt gaagttgttg caaccaagac aggtgcatac
      241 gctaagaagg gcgcagctct tgaagattca agcttagcta aggctgagaa caacggtaac
      301 ggtgttttcg ttgcttcagg tgctgatgat gattttggtg ctgacggtgt aatgtggact
      361 gttgaactca aggttccagc agacgctaag gctggcgatg tatatccaat cgacgttgct
      421 tatcagtggg atccttcaaa gggtgacctt ttcacagata acaaggactc agcacagggc
      481 aagctgatgc aggcttactt cttcacacag ggtatcaagt catcttctaa tccttcaaca
      541 gatgagtacc tcgtaaaggc taacgctaca tatgctgatg gttacattgc aatcaaggct
      601 ggtgagccag gcagtgtagt accatcaaca cagcctgtaa caacaccacc tgcaacaaca
      661 aaaccacctg caacaacaat accgccgtca gatgatccga atgccatggc caatacaccg
      721 gtatcaggca atttgaaggt tgaattctac aacagcaatc cttcagatac tactaactca
      781 atcaatcctc agttcaaggt tactaatacc ggaagcagtg caattgattt gtccaaactc
      841 acattgagat attattatac agtagacggt cagaaagatc agaccttctg gagtgaccat
      901 gctgcaataa tcggcagtaa cggcagctac aacggaatta cttcaaatgt aaaaggaaca
      961 tttgtaaaaa tgagttcctc aacaaataac gcagacacct accttgaaat aagctttaca
     1021 ggcggaactc ttgaaccggg tgcacatgtt cagatacaag gtagatttgc aaagaatgac
     1081 tggagtaact atacacagtc aaatgactac tcattcaagt ctgcttcaca gtttgttgaa
     1141 tgggatcagg taacagcata cttgaacggt gttcttgtat ggggtaaaga acccggtgag
     1201 ctcaagcttc ctaggtctag gcaccaccac caccaccacg gatccctgga agttctgttc
     1261 cagggtccgc tgccggaaac cggcggctaa
//
