LOCUS       sequence-176269-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 
            cassette, and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 371 pTol2-fli1ep:MTDest
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             3425..3454
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3464..3493
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3503..3532
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3542..3571
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3581..3610
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3644..3673
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    3701..3825
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3850..3855,3856..3873,3874..3880)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3934..4593
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4000..4019)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4935..5240
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5119..5138
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5281..5405)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    5449..5583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5473..5492)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5527..5546
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7724..7911,7912..7923)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7957..7973)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7957..7973)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8010..8030)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8010..8028)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8049..8065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8049..8065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8062..8084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8073..8089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8097..8103,8104..8121,8122..8127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8142..8163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8280..8297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8451..9039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8531..8550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9210..10001,10002..10070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9833..9852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10071..10175)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 taattcgccc ttggagatct catctttgac ccataaacat acactaaaac ccaacagtca
     1381 actttatcaa atgaaatgag tgtaattaac tcaaaattta ctgaatgttg attctactca
     1441 ttcgaaaaga gttttaaact cagtgttgaa ggtagtgagt tcattaaata cctcattact
     1501 tcaacttaac tggagtaagt tcatggtact cacatagatt agtttagctc aaatggtttg
     1561 tagcagtcgg ttccctcaag cagtttgagt tgccttaact ttttgggttt tacagtactc
     1621 agttggtttg agttcacttc acttattggg ttcaacatgt gctcaaattg catcgtttaa
     1681 tcaaatagat taagttcaca gttactcatt aggattagtt tttgaactta aatggtttgt
     1741 tgcagtcggt ttcctcaaac ggtttgagtt tccataactt tatgggtttt tcagtgtagt
     1801 aaacatatgc atccacagat tataatagta acctgcatgt tttttccttc acaatttgac
     1861 acagatacct cacatgattg acttttaact gtacacttta gtcgacaatg agaagggtgt
     1921 ctcaatctat gggtatttcc tgttccacac aatctggttt cctgtcaggt ttccttttcc
     1981 tcctgaaaca aaagacagga atgccctctg tataaaatag cacagcgctt ttaatgggtc
     2041 tctgtacatc ccgacctcat gacgcatctt ctgcgggaca gctcaatcac gatcaagttg
     2101 gcttatgaaa acagtttttt gtccccctgt taagttaaaa gcacggtagc atacatgcat
     2161 tattcatcat gcagcatatt cagcagtaca aaagatgagc tcttgacaca cctcagcgta
     2221 aaatctctat tccagcgtaa tcctgcggtt ctggatggtt aagcgagcct tgcaaacacg
     2281 gccaatttat agccatgttg aaaatagcag ctttgtgagc atttcgacct caatgtacca
     2341 agaatgcgtg ctcggtaact gaaaaagagg gggtggagat tttggattta gagatggaat
     2401 cacatgcacg ctgtatatgc tatataaaaa tagcaggcat ttaatgcatg caaaatatgt
     2461 ttcgacacta aacactacaa gtcggaaata tatctatttt tcttttttta acgaaatgag
     2521 acatttaatg cgcagtaaat gtagtctaac acgcacaata ttcctattat tgtccatgaa
     2581 aaatgtgagg tcatatgcta ctgatttaaa gaaaatgttg aacaggcaca gcacacaaga
     2641 ggtatcagcc tctctatatg gtccatacag caccaggctc gctgcgttca tgtgcattca
     2701 gaggcgcgca cacgtgtgga gcagacagac tcgacgcagc gggctttgct ttttattcat
     2761 ggaagcccat tttagaagaa gctcgggcag actcgtcaca ttcttcactt caccataggt
     2821 agatcaaatc aaagttattg gatataacgg aacaatattc atcatattat gagattatta
     2881 ttaagaccga gtgcattaga catattttgt atttcgatcg ttggaaaaag tatatatttt
     2941 attttagcct taaactatgg ttagcctata ttaatcagca taatctgtta tttcagacgt
     3001 tgtattaatg attatttcta gcacagcttt gatgcatcaa tgcaatgttt ctacaaaccc
     3061 gccttgttta atgtcggaaa aaaagcgcaa caaatgtaaa gagttggcag gcattctgta
     3121 aatatttcca ggcaaaaaca tgcacaaagg aaagaaacac cattgttttg gtgtcttatc
     3181 ttcgctggac aaatatcctg ctttgggtgc cgtaatgaag gcagggccag tgacacacac
     3241 acatacacac acacacatac acgcatacac ggcttccttc tcaagcgcag tgacttcact
     3301 tcccaaattt agaggaaaaa aaaccatccg gcgaaactgt ctctgtctct ccgccacata
     3361 tcgggggctg gaattaattc agacgcgaag ggcgaattat cgctagcatc gatttaaagc
     3421 tatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc
     3481 tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat
     3541 ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga
     3601 agaggacttg aatgaaatgg agagcttggg cgacctcacc atggagcaaa agctcatttc
     3661 tgaagaggac ttgaatttag agatctcgaa ttcaaggatc acaagtttgt acaaaaaagc
     3721 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3781 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3841 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3901 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3961 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     4021 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     4081 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     4141 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     4201 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     4261 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     4321 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     4381 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     4441 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     4501 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     4561 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc
     4621 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     4681 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     4741 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     4801 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     4861 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     4921 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     4981 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     5041 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     5101 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     5161 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     5221 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     5281 catagtgact ggatatgttg tgttttacag cattatgtag tctgtttttt atgcaaaatc
     5341 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     5401 gtggtgatcc tctcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg
     5461 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5521 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5581 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5641 ttttaattcg cggccgcggc gccaatgcat tgggccctcg agcatatgag atctgtcccg
     5701 atctttctct tctgtgctgt caatttagag tagtttttga ggtaatttgg gtgcattctc
     5761 tgtaaaaaca acaaataaat atttatattg tatgcaataa atgtaacaat tacacataca
     5821 ctctctgatt acagcattaa agggttagtt cacccaaaaa tgaaaagatg tcattaatga
     5881 ctcaccctca tgtcgttcca agcccgtaac acctccgttc atcttcggaa cacagtttaa
     5941 gatattttag atttagtccg agagctttct gtccctccac tgaaaatgta tgtacggtat
     6001 actgtccatg tccagaaagg taataaaaac atcaaagtag tccatgtgac atcagtgggt
     6061 taggtagaat tatttgaagc atcgaaaata cattttggtc caaaaataac aaaacctacg
     6121 actttattca gcattgtagc gtcactgcga agtcgtgaac gcggattgac aacagacccg
     6181 gaagagaata caatgccgaa taaagtcgta ggttttgtta tttttggacc aaaatgtatt
     6241 cgatgcttca aaaaattcta actaacccac tgatgtcaca tggactactt tgatgttttt
     6301 attacctttc tggacatgga cagtataccg tacatacatt ctcaatggag gcacagaaag
     6361 ctctcggact aaatctaaaa tatcttaaac tgtgttctga agatgaacgg aggtcttacg
     6421 ggcttggaac gacatgaggg cgagtcatta atgacattat tttcattttt gggtgaacta
     6481 accctttaat aaaactgaca tttgcagcca attgcacact ttataatgtt aaatacttgt
     6541 tgattttaga aatacagtag catttagtga caatcaaaca ttaattttat ttctgattat
     6601 attgacaaaa acacaggact gtaaggctga catgacaggg tgaattgatt tcattacatt
     6661 agttttgatg gtaacacttt tacaataagg ttcgttagtt aactacatta gttaacatga
     6721 actaataatg aactgcacgt atacatgtta atttcaacat ttactaatac tttatatctt
     6781 gttaacatta gttaatgcac tgtgaactaa catgaacaaa caatgaacat ctgtatttct
     6841 attaactaac gttaacaaag attaacagat acagtaacaa atgtattgct catggttatt
     6901 taatgttagt taatacatga actaatcaac ctcattgtaa agtgttaccg ttttgacaat
     6961 acaacatgtt tggaaaaaaa tttctcgctt ggttttcttg tgttttgcat gaattcaaac
     7021 acaatgagag cacggaatga taatgattat tcaaagctgc aataggcgcc aaatttcccg
     7081 tggacctaac gttaccaatt acaaatgcat tttagcattt tattacgtga gccttatatt
     7141 gtcaatcgca tgcattagtg aaaataggcc tactacacat ataaaaggcg cgccatcaac
     7201 gtcaaaacat gcacacccaa aaaaaaaaaa aaaaaaaaaa aaaaaaacta tcagtaaaac
     7261 aaaatactta atgtacttac ctcaatatgc ttccttaggt ttgatggcga acttttgaag
     7321 gccgatattt ctttattaag cgggagacag aggacacatt tcatcttgaa tgaatcttta
     7381 tttacaccac ttaaagaaaa gaattcgcga agatacggcc acgggtgctc ttgatcctgt
     7441 ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc ctccattttc
     7501 ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg tgcattgtgg
     7561 taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga ccaatttcat
     7621 ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa ctgggcatca
     7681 gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat ctccaaaaaa
     7741 taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt ttttctaaaa
     7801 aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt aaagtaaaaa
     7861 tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta ctttacacct
     7921 ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct agagcggccg
     7981 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     8041 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8101 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8161 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8221 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8281 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8341 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8401 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8461 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8521 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8581 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8641 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8701 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8761 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8821 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8881 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8941 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9001 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9061 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9121 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9181 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9241 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9301 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9361 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9421 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9481 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9541 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9601 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9661 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9721 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9781 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9841 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9901 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9961 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10021 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10081 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10141 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtg
//
