LOCUS       sequence-17627-e         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   DiRenzo J, Signoretti S, Nakamura N, Rivera-Gonzalez R, Sellers W, 
            Loda M, Brown M
  TITLE     Growth factor requirements and basal phenotype of an immortalized 
            mammary epithelial cell line.
  JOURNAL   Cancer Res. 2002 Jan 1. 62(1):89-98.
  PUBMED    11782364
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2002 Jan 1. 62(1):89-98."
COMMENT     Sequence Label: 1072 pBabe puroL myr Akt K179M T308A S473A
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..46
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             50..76
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     CDS             56..82
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 caccatgggg agcagcaaga gcaagcccaa ggaccccagc cagcgcgcct acgcctacga
       61 cgtgcccgac tacgcctccc tcggatccat gagcgacgtg gctattgtga aggagggttg
      121 gctgcacaaa cgaggggagt acatcaagac ctggcggcca cgctacttcc tcctcaagaa
      181 tgatggcacc ttcattggct acaaggagcg gccgcaggat gtggaccaac gtgaggctcc
      241 cctcaacaac ttctctgtgg cgcagtgcca gctgatgaag acggagcggc cccggcccaa
      301 caccttcatc atccgctgcc tgcagtggac cactgtcatc gaacgcacct tccatgtgga
      361 gactcctgag gagcgggagg agtggacaac cgccatccag actgtggctg acggcctcaa
      421 gaagcaggag gaggaggaga tggacttccg gtcgggctca cccagtgaca actc
//
