LOCUS       sequence-176281-        5909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length human MAGE-A11 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Bai S, He B, Wilson EM
  TITLE     Melanoma antigen gene protein MAGE-11 regulates androgen receptor 
            function by modulating the interdomain interaction.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(4):1238-57.
  PUBMED    15684378
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(4)"; pages: "1238-57"
COMMENT     Sequence Label: pSG5-MAGE-A11
FEATURES             Location/Qualifiers
     source          1..5909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2771..2892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2808..2827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2862..2881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2926..2943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3097..3685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3177..3196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3856..4647,4648..4716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4479..4498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4717..4821)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4889..4907
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4945..4967)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5067..5086
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5153..5608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5290..5311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5502..5521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5734..5756
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5748..5765
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5749..5765
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5802..5822)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttcgcggccg cgtcgacgag tggcagaggg cagcgggtcc aggctccatg aggaggcaag
      961 ccttgggaat ctgagggatg gagactcagt tccgcagagg gggtctgggg tgcagccctg
     1021 ccagcatcaa gaggaagaag aagagggagg actcaggaga ctttggactc caggtgagca
     1081 ctatgttctc agaggacgac ttccagtcaa cagaaagagc cccatatggt ccacaactac
     1141 agtggtccca ggatctgcca agagtccagg tttttagaga acaggccaac ctggaggaca
     1201 ggagtcccag gagaacccag aggatcactg gaggagaaca agtgctgtgg ggccccatca
     1261 cccagatatt tcccacagtt cggcctgctg acctaaccag agtcatcatg cctcttgagc
     1321 aaagaagtca gcactgcaag cctgaggaag gccttcaggc ccaagaagaa gacctgggcc
     1381 tggtgggtgc acaggctctc caagctgagg agcaggaggc tgccttcttc tcctctactc
     1441 tgaatgtggg cactctagag gagttgcctg ctgctgagtc accaagtcct ccccagagtc
     1501 ctcaggaaga gtccttctct cccactgcca tggatgccat ctttgggagc ctatctgatg
     1561 agggctctgg cagccaagaa aaggaggggc caagtacctc gcctgacctg atagaccctg
     1621 agtccttttc ccaagatata ctacatgaca agataattga tttggttcat ttattgctcc
     1681 gcaagtatcg agtcaagggg ctgatcacaa aggcagaaat gctggggagt gtcatcaaaa
     1741 attatgagga ctactttcct gagatattta gggaagcctc tgtatgcatg caactgctct
     1801 ttggcattga tgtgaaggaa gtggacccca ctagccactc ctatgtcctt gtcacctccc
     1861 tcaacctctc ttatgatggc atacagtgta atgagcagag catgcccaag tctggcctcc
     1921 tgataatagt cctgggtgta atcttcatgg aggggaactg catccctgaa gaggttatgt
     1981 gggaagtcct gagcattatg ggggtgtatg ctggaaggga gcacttcctc tttggggagc
     2041 ccaagaggct ccttacccaa aattgggtgc aggaaaagta cctggtgtac cggcaggtgc
     2101 ccggcactga tcctgcatgc tatgagttcc tgtggggtcc aagggcccac gctgagacca
     2161 gcaagatgaa agttcttgag tacatagcca atgccaatgg gagggatccc acttcttacc
     2221 catccctgta tgaagatgct ttgagagagg agggagaggg agtctgagca tgagatgcaa
     2281 ccagggccag cgggcaggga aatgggccaa tgcatgcttc agggccacac ccagcagttt
     2341 ccctgtcctg tgtgaaatca ggcccattct tccctctgtg tttgatgaga gaagtcagtg
     2401 ttctcagtag tagaaggcac agtgaatgga agggaacaca ttgtatactg cctttaggtt
     2461 tctcttccat cgggtgactt ggagatttct ttttgtttcc ctttggtaat tttcaaatat
     2521 tgttcctgta ataaaagttt tagttagctt caacatctaa gtgtatggat gatactgacc
     2581 acacatgttg ttttgcttat ccatttcaag tgcaagtgtt tgccattttg taaaacattt
     2641 tgggaaatct tccatcttgc tgtgatttgc aataggtatt ttcttggaga atgtaagaac
     2701 ttaacaataa agctgaactg gtgttgtgaa acagagaaat aaaaggagaa ggtcattaat
     2761 attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2821 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2881 caatgtatct tatcatgtct ggtcgactct agactcttcc gcttcctcgc tcactgactc
     2941 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3001 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3841 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3901 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3961 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4021 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4081 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4141 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4201 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4261 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4321 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4381 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4441 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4501 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4561 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4621 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4681 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4741 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4801 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4861 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     4921 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4981 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5041 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5101 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     5161 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5221 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     5281 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     5341 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     5401 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     5461 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     5521 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     5581 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     5641 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     5701 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     5761 ccagtgaatt gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     5821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5881 gtcagcaagg aaagtcccca ggctcccca
//
