LOCUS       sequence-176282-        5561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of N-terminal HA-tagged human MAGE-A11 amino 
            acid residues 112-429.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Bai S, Wilson EM
  TITLE     Epidermal-growth-factor-dependent phosphorylation and 
            ubiquitinylation of MAGE-11 regulates its interaction with the 
            androgen receptor.
  JOURNAL   Mol Cell Biol. 2008 Mar;28(6):1947-63. doi: 10.1128/MCB.01672-07. 
            Epub 2008 Jan 22.
  PUBMED    18212060
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01672-07"; journalName: "Mol Cell Biol"; date: 
            "2008-03"; volume: "28"; issue: "6"; pages: "1947-63"
COMMENT     Sequence Label: pSG5HA-MAGE-112-429
FEATURES             Location/Qualifiers
     source          1..5561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      906..915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             918..944
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2423..2544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2460..2479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2514..2533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2578..2595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2749..3337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2829..2848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3508..4299,4300..4368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4131..4150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4369..4473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4541..4559
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4597..4619)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4719..4738
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4805..5260)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4942..4963)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5154..5173
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5386..5408
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5400..5417
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5401..5417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5454..5474)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttaccgccgc catgggctac ccatacgatg ttcctgacta tgcggaattc cctcttgagc
      961 aaagaagtca gcactgcaag cctgaggaag gccttcaggc ccaagaagaa gacctgggcc
     1021 tggtgggtgc acaggctctc caagctgagg agcaggaggc tgccttcttc tcctctactc
     1081 tgaatgtggg cactctagag gagttgcctg ctgctgagtc accaagtcct ccccagagtc
     1141 ctcaggaaga gtccttctct cccactgcca tggatgccat ctttgggagc ctatctgatg
     1201 agggctctgg cagccaagaa aaggaggggc caagtacctc gcctgacctg atagaccctg
     1261 agtccttttc ccaagatata ctacatgaca agataattga tttggttcat ttattgctcc
     1321 gcaagtatcg agtcaagggg ctgatcacaa aggcagaaat gctggggagt gtcatcaaaa
     1381 attatgagga ctactttcct gagatattta gggaagcctc tgtatgcatg caactgctct
     1441 ttggcattga tgtgaaggaa gtggacccca ctagccactc ctatgtcctt gtcacctccc
     1501 tcaacctctc ttatgatggc atacagtgta atgagcagag catgcccaag tctggcctcc
     1561 tgataatagt cctgggtgta atcttcatgg aggggaactg catccctgaa gaggttatgt
     1621 gggaagtcct gagcattatg ggggtgtatg ctggaaggga gcacttcctc tttggggagc
     1681 ccaagaggct ccttacccaa aattgggtgc aggaaaagta cctggtgtac cggcaggtgc
     1741 ccggcactga tcctgcatgc tatgagttcc tgtggggtcc aagggcccac gctgagacca
     1801 gcaagatgaa agttcttgag tacatagcca atgccaatgg gagggatccc acttcttacc
     1861 catccctgta tgaagatgct ttgagagagg agggagaggg agtctgagca tgagatgcaa
     1921 ccagggccag cgggcaggga aatgggccaa tgcatgcttc agggccacac ccagcagttt
     1981 ccctgtcctg tgtgaaatca ggcccattct tccctctgtg tttgatgaga gaagtcagtg
     2041 ttctcagtag tagaaggcac agtgaatgga agggaacaca ttgtatactg cctttaggtt
     2101 tctcttccat cgggtgactt ggagatttct ttttgtttcc ctttggtaat tttcaaatat
     2161 tgttcctgta ataaaagttt tagttagctt caacatctaa gtgtatggat gatactgacc
     2221 acacatgttg ttttgcttat ccatttcaag tgcaagtgtt tgccattttg taaaacattt
     2281 tgggaaatct tccatcttgc tgtgatttgc aataggtatt ttcttggaga atgtaagaac
     2341 ttaacaataa agctgaactg gtgttgtgaa acagagaaat aaaaggagaa ggtcattaac
     2401 gatccagatc ttattaaagc agaacttgtt tattgcagct tataatggtt acaaataaag
     2461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2521 gtccaaactc atcaatgtat cttatcatgt ctggtcgact ctagactctt ccgcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4501 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4561 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4621 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4681 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4741 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     4801 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4861 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4921 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4981 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5041 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5101 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5161 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5221 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     5281 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     5341 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     5401 gtaaaacgac ggccagtgaa ttgtcgactt ctgaggcgga aagaaccagc tgtggaatgt
     5461 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5521 gcatctcaat tagtcagcaa ggaaagtccc caggctcccc a
//
