LOCUS       sequence-176286-        4225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human androgen receptor with 
            added N-terminal VP16 activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Langley E, Kemppainen JA, Wilson EM
  TITLE     Intermolecular NH2-/carboxyl-terminal interactions in androgen 
            receptor dimerization revealed by mutations that cause androgen 
            insensitivity.
  JOURNAL   J Biol Chem. 1998 Jan 2;273(1):92-101.
  PUBMED    9417052
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-01-2"; volume: "273(1)"; pages: "92-101"
COMMENT     Sequence Label: VP16-hAR
FEATURES             Location/Qualifiers
     source          1..4225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1615..1749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1652..1671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1706..1725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1812..1831)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2096..2113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2267..2855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2347..2366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3026..3817,3818..3886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3649..3668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3887..3991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4046..4066)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggcgggagct gtagccccct acggctacac tcggccccct caggggctgg cgggccagga
       61 aagcgacttc accgcacctg atgtgtggta ccctggcggc atggtgagca gagtgcccta
      121 tcccagtccc acttgtgtca aaagcgaaat gggcccctgg atggatagct actccggacc
      181 ttacggggac atgcgtttgg agactgccag ggaccatgtt ttgcccattg actattactt
      241 tccaccccag aagacctgcc tgatctgtgg agatgaagct tctgggtgtc actatggagc
      301 tctcacatgt ggaagctgca aggtcttctt caaaagagcc gctgaaggga aacagaagta
      361 cctgtgcgcc agcagaaatg attgcactat tgataaattc cgaaggaaaa attgtccatc
      421 ttgtcgtctt cggaaatgtt atgaagcagg gatgactctg ggagcccgga agctgaagaa
      481 acttggtaat ctgaaactac aggaggaagg agaggcttcc agcaccacca gccccactga
      541 ggagacaacc cagaagctga cagtgtcaca cattgaaggc tatgaatgtc agcccatctt
      601 tctgaatgtc ctggaagcca ttgagccagg tgtagtgtgt gctggacacg acaacaacca
      661 gcccgactcc tttgcagcct tgctctctag cctcaatgaa ctgggagaga gacagcttgt
      721 acacgtggtc aagtgggcca aggccttgcc tggcttccgc aacttacacg tggacgacca
      781 gatggctgtc attcagtact cctggatggg gctcatggtg tttgccatgg gctggcgatc
      841 cttcaccaat gtcaactcca ggatgctcta cttcgcccct gatctggttt tcaatgagta
      901 ccgcatgcac aagtcccgga tgtacagcca gtgtgtccga atgaggcacc tctctcaaga
      961 gtttggatgg ctccaaatca ccccccagga attcctgtgc atgaaagcac tgctactctt
     1021 cagcattatt ccagtggatg ggctgaaaaa tcaaaaattc tttgatgaac ttcgaatgaa
     1081 ctacatcaag gaactcgatc gtatcattgc atgcaaaaga aaaaatccca catcctgctc
     1141 aagacgcttc taccagctca ccaagctcct ggactccgtg cagcctattg cgagagagct
     1201 gcatcagttc acttttgacc tgctaatcaa gtcacacatg gtgagcgtgg actttccgga
     1261 aatgatggca gagatcatct ctgtgcaagt gcccaagatc ctttctggga aagtcaagcc
     1321 catctatttc cacacccagt gaagcattgg aaaccctatt tccccacccc agctcatgcc
     1381 ccctttcaga tgtcttctgc ctgttataac tctgcactac tcctctgcag tgccttgggg
     1441 aatttcctct attgatgtac agtctgtcat gagcttgcat gcctgcaggt cgactctaga
     1501 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
     1561 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1621 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1681 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1741 gtctggatcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     1801 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     1861 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     1921 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     1981 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat atgcggtgtg
     2041 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     2101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     3361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     3661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacc
     4021 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4081 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaggaaagtc
     4141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4201 agtcccgccc ctaactccgc ccagt
//
