LOCUS       sequence-176289-       14197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MESA protease chain with V2-MESA ectodomain, 35 extracellular 
            linkers, a flag tag, and Tev protease, MS2-P65-HSF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 14197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V2-MESA-35F-Tev-MS2-p65-HSF1
FEATURES             Location/Qualifiers
     source          1..14197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(177..765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(257..276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(936..1727,1728..1796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1559..1578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1797..1901)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1949..2083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2251..2884
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2931..3056
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3553..3786
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3971..4015
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4313..4430
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4495..4869)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      4866..4875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4879..4897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4889..4936)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        5041..5344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5345..5548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5499..5519
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5545..5569
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6592..7296
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="ESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKH
                     LFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQR
                     EERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEE
                     PFQPVKEATQLMN"
     primer_bind     7575..7595
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7634..7643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7643..8029
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8084..8104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8120..8662
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8687..9058
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             9074..9127
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9131..10150
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    10190..10778
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10243..10263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10661..10672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10781..10797)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10782..10798)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10983..11216
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11317..11334)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11323..11547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11593..12021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11680..11699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11890..11911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12030..12050)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12035..12364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12215..12350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12277..12296
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12413..13438
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             12413..13438
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    13568..13689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13738..13754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13738..13754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13751..13773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13762..13778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13786..13792,13793..13810,13811..13816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13831..13852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
       61 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1921 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
     1981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     2101 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     2161 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     2221 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     2281 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     2341 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     2401 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     2461 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     2521 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     2581 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     2641 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     2701 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2761 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2821 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2881 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     2941 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     3001 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     3061 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     3121 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     3181 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     3241 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     3301 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     3361 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     3421 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3481 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3541 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3601 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3661 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3721 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3781 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3841 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3901 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3961 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4021 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4081 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4141 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4201 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4261 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     4321 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4381 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4441 acagggacag cagagatcca gtttggttag taccgggccc gctctagtcc ggaatcagtc
     4501 ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     4561 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     4621 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     4681 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     4741 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     4801 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     4861 cttggccatg gtggccctcc tatagtgagt cgtattatac tatgccgata tactatgccg
     4921 atgattaatt gtcaacacgt gctgcaggtc cgaggttcta gacgtattac cgccatgcat
     4981 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     5041 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     5101 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     5161 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     5221 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     5281 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     5341 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     5401 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     5461 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     5521 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     5581 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctgccacca
     5641 gctagcgcca tcatggcttg gaccagctta attcttagct tgcttgccct gtgcagcggt
     5701 gcatcatctg acatacagat gactcagtca cctagcagtc tgtctgcttc agtcggtgat
     5761 agggtaacta tcacctgtcg ggcatctcag gtgatcagaa ggtctctggc ctggtatcag
     5821 cagaagcccg gcaaggctcc taagttgctt atctatgcag cttcaaactt ggccagtggg
     5881 gtgcctagcc ggtttagcgg aagtggttct ggcacagact tcactttgac aatcagttcc
     5941 ctccaaccgg aggatttcgc tacctactac tgtcagcagt ccaacaccag ccctctgacg
     6001 tttggacagg gcactaaagt ggagattaag aggggaggcg gaggctccgg tgggggaggc
     6061 tctggtggtg gaggtagcga ggttcagctg gtggaaagtg gtggcggact agtacagcca
     6121 ggcggctccc tgcggctgag ttgcgccgcg agtggctttt ctataaacgg atcctggatc
     6181 ttctgggtca ggcaagcccc tgggaaagga ttggagtggg ttggagccat ttggcctttc
     6241 ggtgggtata ctcactatgc agatagcgtg aaagggaggt tcaccatctc tgcagacaca
     6301 agcaagaaca cggcctacct acagatgaac tctctgaggg cagaggacac tgccgtctac
     6361 tattgcgccc gatggggaca ctccacaagc ccttgggcca tggactattg gggacagggg
     6421 acattggtag gcgccggatc tggaggagac tacaaggacg atgacgacaa cggcggatcg
     6481 ggggggtctg gtggtagtgg cggttcgggg ggaggtaccg gtctggtcgt ggttgctgga
     6541 gtcctgtttt gttatggctt gctagtgaca gtggctcttt gtgttgaaaa cgagagcttg
     6601 tttaaggggc cgcgtgatta caacccgata tcgagcacca tttgtcattt gacgaatgaa
     6661 tctgatgggc acacaacatc gttgtatggt attggatttg gtcccttcat cattacaaac
     6721 aagcacttgt ttagaagaaa taatggaaca ctgttggtcc aatcactaca tggtgtattc
     6781 aaggtcaaga acaccacgac tttgcaacaa cacctcattg atgggaggga catgataatt
     6841 attcgcatgc ctaaggattt cccaccattt cctcaaaagc tgaaatttag agagccacaa
     6901 agggaagagc gcatatgtct tgtgacaacc aacttccaaa ctaagagcat gtctagcatg
     6961 gtgtcagaca ctagttgcac attcccttca tctgatggca tattctggaa gcattggatt
     7021 caaaccaagg atgggcagtg tggcagtcca ttagtatcaa ctagagatgg gttcattgtt
     7081 ggtatacact cagcatcgaa tttcaccaac acaaacaatt atttcacaag cgtgccgaaa
     7141 aacttcatgg aattgttgac aaatcaggag gcgcagcagt gggttagtgg ttggcgatta
     7201 aatgctgact cagtattgtg ggggggccat aaagttttca tggtgaaacc tgaagagcct
     7261 tttcagccag ttaaggaagc gactcaactc atgaattagg gcgcgccgct gcaaagatgg
     7321 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     7381 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     7441 aagttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     7501 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     7561 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     7621 gtcgtgacgt acggccacca tggcttcaaa ctttactcag ttcgtgctcg tggacaatgg
     7681 tgggacaggg gatgtgacag tggctccttc taatttcgct aatggggtgg cagagtggat
     7741 cagctccaac tcacggagcc aggcctacaa ggtgacatgc agcgtcaggc agtctagtgc
     7801 ccagaagaga aagtatacca tcaaggtgga ggtccccaaa gtggctaccc agacagtggg
     7861 cggagtcgaa ctgcctgtcg ccgcttggag gtcctacctg aacatggagc tcactatccc
     7921 aattttcgct accaattctg actgtgaact catcgtgaag gcaatgcagg ggctcctcaa
     7981 agacggtaat cctatccctt ccgccatcgc cgctaactca ggtatctaca gcgctggagg
     8041 aggtggaagc ggaggaggag gaagcggagg aggaggtagc ggacctaaga aaaagaggaa
     8101 ggtggcggcc gctggatccc cttcagggca gatcagcaac caggccctgg ctctggcccc
     8161 tagctccgct ccagtgctgg cccagactat ggtgccctct agtgctatgg tgcctctggc
     8221 ccagccacct gctccagccc ctgtgctgac cccaggacca ccccagtcac tgagcgctcc
     8281 agtgcccaag tctacacagg ccggcgaggg gactctgagt gaagctctgc tgcacctgca
     8341 gttcgacgct gatgaggacc tgggagctct gctggggaac agcaccgatc ccggagtgtt
     8401 cacagatctg gcctccgtgg acaactctga gtttcagcag ctgctgaatc agggcgtgtc
     8461 catgtctcat agtacagccg aaccaatgct gatggagtac cccgaagcca ttacccggct
     8521 ggtgaccggc agccagcggc cccccgaccc cgctccaact cccctgggaa ccagcggcct
     8581 gcctaatggg ctgtccggag atgaagactt ctcaagcatc gctgatatgg actttagtgc
     8641 cctgctgtca cagatttcct ctagtgggca gggaggaggt ggaagcggct tcagcgtgga
     8701 caccagtgcc ctgctggacc tgttcagccc ctcggtgacc gtgcccgaca tgagcctgcc
     8761 tgaccttgac agcagcctgg ccagtatcca agagctcctg tctccccagg agccccccag
     8821 gcctcccgag gcagagaaca gcagcccgga ttcagggaag cagctggtgc actacacagc
     8881 gcagccgctg ttcctgctgg accccggctc cgtggacacc gggagcaacg acctgccggt
     8941 gctgtttgag ctgggagagg gctcctactt ctccgaaggg gacggcttcg ccgaggaccc
     9001 caccatctcc ctgctgacag gctcggagcc tcccaaagcc aaggacccca ctgtctcctg
     9061 tacaggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     9121 tggcccaacc atgaaaaagc ctgaactcac cgctacctct gtcgagaagt ttctgatcga
     9181 aaagttcgac agcgtctccg acctgatgca gctctccgag ggcgaagaat ctcgggcttt
     9241 cagcttcgat gtgggagggc gtggatatgt cctgcgggtg aatagctgcg ccgatggttt
     9301 ctacaaagat cgctatgttt atcggcactt tgcatccgcc gctctcccta ttcccgaagt
     9361 gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     9421 tgtcaccttg caagacctgc ctgaaaccga actgcccgct gttctccagc ccgtcgccga
     9481 ggccatggat gccatcgctg ccgccgatct tagccagacc agcgggttcg gcccattcgg
     9541 acctcaagga atcggtcaat acactacatg gcgcgatttc atctgcgcta ttgctgatcc
     9601 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcctccg tcgcccaggc
     9661 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     9721 cgatttcggc tccaacaatg tcctgaccga caatggccgc ataacagccg tcattgactg
     9781 gagcgaggcc atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     9841 ctggttggct tgtatggagc agcagacccg ctacttcgag cggaggcatc ccgagcttgc
     9901 aggatctcct cggctccggg cttatatgct ccgcattggt cttgaccaac tctatcagag
     9961 cttggttgac ggcaatttcg atgatgcagc ttgggctcag ggtcgctgcg acgcaatcgt
    10021 ccggtccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg ctgccgtctg
    10081 gaccgatggc tgtgtggaag tgctcgccga tagtggaaac agacgcccca gcactcgtcc
    10141 tagggcaaag gatctgcagt aatgagaatt cgatatcaag cttatcggta atcaacctct
    10201 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    10261 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    10321 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    10381 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    10441 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    10501 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    10561 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
    10621 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    10681 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
    10741 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
    10801 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
    10861 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
    10921 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
    10981 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
    11041 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    11101 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    11161 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
    11221 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
    11281 tgagaggcct tgacattgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
    11341 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    11401 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    11461 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    11521 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    11581 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    11641 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    11701 cctttctcgt cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    11761 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    11821 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    11881 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    11941 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    12001 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
    12061 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    12121 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    12181 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    12241 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12301 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    12361 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgatgaaaaa
    12421 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
    12481 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
    12541 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
    12601 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
    12661 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
    12721 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
    12781 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
    12841 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
    12901 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
    12961 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
    13021 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
    13081 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
    13141 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
    13201 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
    13261 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
    13321 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
    13381 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
    13441 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    13501 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    13561 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13681 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    13741 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    13801 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    13861 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    13921 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    13981 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    14041 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    14101 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    14161 tgacgagcat cacaaaaatc gacgctcaag tcagagg
//
