LOCUS       sequence-176300-        9906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively active NFAT1, mutated to interfere selectively with 
            the NFAT:AP-1 interaction. Co-expresses EGFP for selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, 
            Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, 
            Mempel TR, Lahdesmaki H, Hogan PG, Rao A
  TITLE     The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T
            Cells.
  JOURNAL   Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006.
  PUBMED    25680272
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006."
COMMENT     Sequence Label: CA-RIT-NFAT1 (IRES-GFP)
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    12..564
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             join(576..578,579..581,582..1295)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(621..642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(882..901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1229..1250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1342..1857
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2019..2035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2043..2049,2050..2067,2068..2073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2088..2109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2226..2243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2397..2985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2477..2496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3156..3947,3948..4016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3779..3798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4017..4121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4189..4207
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4245..4267)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4367..4386
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4580..4602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4730..4749
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             4949..5465
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    5529..5870
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             5937..6353
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6283..6305
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6321..6337
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    6378..6402
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6447..6536
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    complement(9363..9387)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
       61 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      121 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      181 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      241 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      301 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      361 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
      421 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
      481 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      541 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
      601 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      661 gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      721 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      781 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      841 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      901 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      961 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1021 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc
     1081 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1141 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1201 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1261 ggatcactct cggcatggac gagctgtaca agtaatgaga taaaataaaa gattttattt
     1321 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     1381 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     1441 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     1501 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     1561 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     1621 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     1681 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     1741 ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgacttg tggtctcgct
     1801 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg
     1861 taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat
     1921 cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat
     1981 tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca
     2041 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2101 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2161 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2221 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2281 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2341 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2401 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2461 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2521 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2581 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2641 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2701 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2761 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2821 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2881 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2941 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3001 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3061 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3121 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3181 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3241 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3301 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3361 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3421 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3481 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3541 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3601 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3661 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3721 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3781 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3841 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3901 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3961 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4021 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4081 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4141 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4201 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     4261 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     4321 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     4381 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4441 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4501 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4561 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg
     4621 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     4681 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     4741 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     4801 cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     4861 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
     4921 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     4981 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     5041 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     5101 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct
     5161 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     5221 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     5281 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac
     5341 tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat ccgaatcgtg
     5401 gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct cgggggtctt
     5461 tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg acccccccgc
     5521 cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg
     5581 ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc
     5641 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
     5701 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
     5761 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
     5821 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
     5881 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
     5941 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     6001 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     6061 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     6121 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     6181 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     6241 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     6301 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaatta
     6361 gatctctcga ggtcatcaca agtttgtaca aaaaagcagg cttcgaagga gatagaacca
     6421 attcttaagc ttccaccatg atcttttacc catacgatgt tcctgactat gcgggctatc
     6481 cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc
     6541 agtgcggccg cgacgtcccg gagccgcagc ccgaccccga tggcggggac ggccccggcc
     6601 acgagcccgg gggcagtccc caagacgagc tggacttttc catcctcttc gattatgact
     6661 atctgaaccc tatcgaagaa gaaccgatcg cacataaggc catcagctca ccctccggac
     6721 tcgcataccc ggatgatgtc ctggactatg gcctcaagcc atgcaacccc cttgccagtc
     6781 tctctggcga gccccctggc cggttcggag agccggatag tatagggttc cagaactttc
     6841 tgagcccggt caagccagca ggggcttcgg gcccgagccc tcggatcgag atcactccat
     6901 cccacgaact gatgcaggca gggggggccc tccgtgggag agacgccggc ctgtcccccg
     6961 agcagccggc cctggccctg gccggcgtgg ccgccagccc gaggttcaca ctgcccgtgc
     7021 ccggctacga gggctaccgc gagccgcttt gcttgagccc cgctgcagca ggcgcagcag
     7081 ccgcattcat ttctgacacc ttctccccct acacctcgcc ctgcgtctca cccaataacg
     7141 ccgggcccga cgacctgtgt ccccagtttc aaaacatccc tgctcattat gccccccgga
     7201 ccgctccaat aatggcacct cgaaccagcc tcgccgagga cagctgcctg ggccgacact
     7261 cgcccgtgcc ccgtccggca tcccgctcct cctcacccgg tgccaagcgg aggcattcgt
     7321 gcgcagaggc tttggttgct cctctgcccg cagccgcacc ccagcgcgcc cggagccccg
     7381 cgccacagcc cgcgcctcac gtggcaccgc aggacgacag catccccgct gggtaccccc
     7441 ccacggccgg ctctgctgtt ctcatggatg ccctcaacac cctggccacc gactcgccct
     7501 gcgggatccc ctccaagata tggaagacca gtcctgaccc gacgcctgtg tccaccgctc
     7561 cgtccaaggc tggcctggcc cgccacatct accctactgt ggagttcctg gggccatgtg
     7621 agcaggagga gaggaggaat tccgctccag agtccatcct gctggtacca cctacttggc
     7681 ccaagcagtt ggtgccggcc attcccatct gcagcatccc tgtgactgca tccctcccac
     7741 cactcgagtg gccactctcc aatcagtcgg gctcctatga gctacggatt gaggtccaac
     7801 ccaagcccca tcaccgggcc cactatgaga cggagggcag ccgtggcgct gtcaaagccc
     7861 caacaggagg acaccctgtg gtgcagctcc acggctacat ggagaacaag cctctggggc
     7921 ttcagatctt cattgggaca gcagatgagg cggccctaaa gccgcacgcc ttctaccaag
     7981 tacacaggat cactgggaaa acggtcacca ccacgagcta tgagaagatc gtaggcaaca
     8041 ccaaggtcct ggagatcccc ctggagccaa agaacaacat gagagccacc atcgactgtg
     8101 caggcatcct gaagctccga aacgctgaca tcgagctgcg gaagggcgag ggggacatcg
     8161 gcaggaagaa cacgcgtgtg cgcctggtgt tccgcgtgca cgtcccagag cccagtgggc
     8221 gcatcgtctc cctgcaggct gcgtccaacc ccatcgagtg ctctcagcgc tctgcccacg
     8281 agctgcccat ggtggagaga caagacatgg acagctgcct ggtctacggg ggccagcaga
     8341 tgatcctcac gggccagaac ttcacagcgg agtccaaggt tgtgttcatg gagaagacta
     8401 cagatgggca gcagatttgg gagatggaag ctacggtgga taaagacaag agccagccta
     8461 acatgctttt tgttgagatc cccgagtatc ggaacaagca catccgcgtg cccgtgaaag
     8521 tcaacttcta cgtcatcaac ggaaagagga aacgaagtca gccacagcac tttacctacc
     8581 acccagtccc tgccatcaag acagagccca gcgatgagta tgaaccatct ttgatctgca
     8641 gccccgccca tggaggcctg gggagccagc catattaccc acagcaccca atgctggccg
     8701 agtccccctc ctgccttgtg gctaccatgg ccccctgcca acagttccgc tcggggctct
     8761 catcccccga tgctcgctac caacagcaga gccccgcagc tgccctctac cagagaagca
     8821 agagcctgag tcccggcctg ctgggctacc agcagccgtc cctcctggca gcacccttgg
     8881 gtctggctga tgcccaccgc tctgtgctgg tgcatgctgg ttctcagggg caggggcagg
     8941 gctccaccct ccgacacaca tcctcggcca gccagcaggc ctcacccgtg atccactact
     9001 cacccaccaa ccagcagctt cgcggtgggg gtcaccagga gttccagcat atcatgtact
     9061 gtgaaaactt cggccccagc tctgccaggc ctggcccgcc tcccatcaac caaggtcaga
     9121 ggctgagccc gggcgcctac cccacagtca tccaacaaca gactgccccg agccaaagag
     9181 ctgccaaaaa cggacccagt gaccagaagg aagctctgcc cacgggagtg accgtcaaac
     9241 aggaacagaa cctggaccag acctacctgg atgacgttaa tgaaatcatc aggaaggagt
     9301 tttcaggacc tccctcccga aatcagacct agaaacaaac ataatctcgc gacgcaatct
     9361 agacccagct ttcttgtaca aagtggtgat gacggtatcg ataagcttga tatcgaattc
     9421 cgcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     9481 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     9541 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     9601 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     9661 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     9721 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     9781 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     9841 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     9901 tacatg
//
