LOCUS       sequence-176322-       12681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral vector which expresses SNAP tagged TCRbeta
            and other TCR subunits (Jurkat alleles) separated via 2A sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Taylor MJ, Husain K, Gartner ZJ, Mayor S, Vale RD
  TITLE     A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering 
            in Ligand Discrimination.
  JOURNAL   Cell. 2017 Mar 23;169(1):108-119.e20. doi: 
            10.1016/j.cell.2017.03.006.
  PUBMED    28340336
REFERENCE   2  (bases 1 to 12681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-03-23- 
            23"; volume: "169"; issue: "1"; pages: "108-119.e20"
COMMENT     Sequence Label: TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3...
FEATURES             Location/Qualifiers
     source          1..12681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     759..776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(846..866)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        851..1180
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1031..1166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2314..2379
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2509..2529
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2954..3088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2991..3010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3045..3064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3256..3889
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3936..4061
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4558..4791
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4976..5020
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5305..5422
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5575..5982
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6909..6968
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7044..7586
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             8487..8543
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9186..9239
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9800..10388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9853..9873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10271..10282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10391..10407)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10392..10408)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10498..10731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10756..10774)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10757..10774)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11064..11083)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11297..11316)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11416..11438
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11476..11494)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11562..11666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11667..11735,11736..12527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11885..11904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
      901 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     1021 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1081 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1141 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     1201 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     1261 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     1321 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     1381 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     1441 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     1501 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     1561 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     1621 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     1681 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     1741 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     1801 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     1861 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     1921 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     1981 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     2041 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     2101 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     2161 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     2221 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     2281 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     2341 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     2401 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     2461 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     2521 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     2581 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     2641 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     2701 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     2761 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     2821 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     2881 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2941 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3061 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     3121 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     3181 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     3241 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     3301 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     3361 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     3421 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     3481 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     3541 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     3601 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     3661 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     3721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     3901 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     3961 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4021 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4081 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4141 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     4201 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     4261 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     4321 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     4381 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     4441 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4501 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     4561 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     4621 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     4681 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     4741 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     4801 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     4861 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     4921 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     4981 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5041 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5101 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5161 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5221 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     5281 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     5341 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     5401 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     5461 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
     5521 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     5581 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     5641 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     5701 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     5761 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     5821 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     5881 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     5941 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg
     6001 agaattctca cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg ggatctgcca
     6061 ccatgctcct gctgctcgtc ccagtgctgg aggtgatttt taccctggga ggaaccagag
     6121 cccagtcggt gacccagctt ggcagccacg tctctgtctc tgaaggagcc ctggttctgc
     6181 tgaggtgcaa ctactcatcg tctgttccac catatctctt ctggtatgtg caatacccca
     6241 accaaggact ccagcttctc ctgaagtaca catcagcggc caccctggtt aaaggcatca
     6301 acggttttga ggctgaattt aagaagagtg aaacctcctt ccacctgaca aaaccctcag
     6361 cccatatgag cgacgcggct gagtacttct gtgctgtgag tgatctcgaa ccgaacagca
     6421 gtgcttccaa gataatcttt ggatcaggga ccagactcag catccggcca aatatccaga
     6481 accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag tctgtctgcc
     6541 tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct gatgtgtata
     6601 tcacagacaa aactgtgcta gacatgaggt ctatggactt caagagcaac agtgctgtgg
     6661 cctggagcaa caaatctgac tttgcatgtg caaacgcctt caacaacagc attattccag
     6721 aagacacctt cttccccagc ccagaaagtt cctgtgatgt caagctggtc gagaagagct
     6781 ttgaaacaga tacgaaccta aactttcaaa acctgtcagt gattgggttc cgaatcctcc
     6841 tcctgaaagt ggccgggttt aatctgctca tgacgctgcg gctgtggtcc agcgaattcg
     6901 ggtccggcca atgtactaac tacgctttgt tgaaactcgc tggcgatgtt gaaagtaacc
     6961 ccggtcctaa gcttatgcag tcgggcactc actggagagt tctgggcctc tgcctcttat
     7021 cagttggcgt ttgggggcaa gatgacaaag actgcgaaat gaagcgcacc accctggata
     7081 gccctctggg caagctggaa ctgtctgggt gcgaacaggg cctgcaccgt atcatcttcc
     7141 tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg
     7201 gcggaccaga gccactgatg caggccaccg cctggctcaa cgcctacttt caccagcctg
     7261 aggccatcga ggagttccct gtgccagccc tgcaccaccc agtgttccag caggagagct
     7321 ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa gttcggagag gtcatcagct
     7381 acagccacct ggccgccctg gccggcaatc ccgccgccac cgccgccgtg aaaaccgccc
     7441 tgagcggaaa tcccgtgccc attctgatcc cctgccaccg ggtggtgcag ggcgacctgg
     7501 acgtgggggg ctacgagggc gggctcgccg tgaaagagtg gctgctggcc cacgagggcc
     7561 acagactggg caagcctggg ctgggtggtt ccggatcagt ggttatccag tcaccccgcc
     7621 atgaggtgac agagatggga caagaagtga ctctgagatg taaaccaatt tcaggccaca
     7681 actccctttt ctggtacaga cagaccatga tgcggggact ggagttgctc atttacttta
     7741 acaacaacgt tccgatagat gattcaggga tgcccgagga tcgattctca gctaagatgc
     7801 ctaatgcatc attctccact ctgaagatcc agccctcaga acccagggac tcagctgtgt
     7861 acttctgtgc cagcagtttc tcgacctgtt cggctaacta tggctacacc ttcggttcgg
     7921 ggaccaggtt aaccgttgta gaggacctga acaaggtgtt cccacccgag gtcgctgtgt
     7981 ttgagccatc agaagcagaa atctcccaca cccaaaaggc cacactggtg tgcctggcca
     8041 caggcttctt ccccgaccac gtggagctga gctggtgggt gaatgggaag gaggtgcaca
     8101 gtggggtcag cacagacccg cagcccctca aggagcagcc cgccctcaat gactccagat
     8161 actgcctgag cagccgcctg agggtctcgg ccaccttctg gcagaacccc cgcaaccact
     8221 tccgctgtca agtccagttc tacgggctct cggagaatga cgagtggacc caggataggg
     8281 ccaaacccgt cacccagatc gtcagcgccg aggcctgggg tagagcagac tgtggcttta
     8341 cctcggtgtc ctaccagcaa ggggtcctgt ctgccaccat cctctatgag atcctgctag
     8401 ggaaggccac cctgtatgct gtgctggtca gcgcccttgt gttgatggcc atggtcaaga
     8461 gaaaggattt cacgcgtggc tccggcgcca cgaacttctc tctgttaaag caagcaggag
     8521 acgtggaaga aaaccccggt cccggtacca tgcagtcggg cactcactgg agagttctgg
     8581 gcctctgcct cttatcagtt ggcgtttggg ggcaagatgg taatgaagaa atgggtggta
     8641 ttacacagac accatataaa gtctccatct ctggaaccac agtaatattg acatgccctc
     8701 agtatcctgg atctgaaata ctatggcaac acaatgataa aaacataggc ggtgatgagg
     8761 atgataaaaa cataggcagt gatgaggatc acctgtcact gaaggaattt tcagaattgg
     8821 agcaaagtgg ttattatgtc tgctacccca gaggaagcaa accagaagat gcgaactttt
     8881 atctctacct gagggcaaga gtgtgtgaga actgcatgga gatggatgtg atgtcggtgg
     8941 ccacaattgt catagtggac atctgcatca ctgggggctt gctgctgctg gtttactact
     9001 ggagcaagaa tagaaaggcc aaggccaagc ctgtgacacg aggagcgggt gctggcggca
     9061 ggcaaagggg acaaaacaag gagaggccac cacctgttcc caacccagac tatgagccca
     9121 tccggaaagg ccagcgggac ctgtattctg gcctgaatca gagacgcatc ggatccgggt
     9181 ccggcgaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccaa
     9241 ccggtatgaa gtggaaggcg cttttcaccg cggccatcct gcaggcacag ttgccgatta
     9301 cagaggcaca gagctttggc ctgctggacc ccaaactctg ctacctgctg gatggaatcc
     9361 tcttcatcta tggtgtcatt ctcactgcct tgttcctgag agtgaagttc agcaggagcg
     9421 cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc aatctaggac
     9481 gaagagagga gtacgatgtt ttggacaaga gacgtggccg ggaccctgag atggggggaa
     9541 agccgcagag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga
     9601 tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg
     9661 atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc
     9721 aggccctgcc ccctcgctga gcggccgcga ctctagagtc gacctgcagg catgcaagct
     9781 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9841 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9901 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9961 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    10021 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    10081 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10141 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    10201 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    10261 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    10321 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    10381 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
    10441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
    10501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
    10561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
    10741 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
    10801 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
    10861 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
    10921 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
    10981 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
    11041 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
    11101 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
    11161 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
    11221 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
    11281 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
    11341 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
    11401 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
    11461 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
    11521 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    11581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    11641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    11701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    11761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    11821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    11881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    11941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    12001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    12061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    12121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    12181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    12241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    12301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    12361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    12421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    12481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    12541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    12601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    12661 gagcgtcaga ccccgtagaa a
//
