LOCUS       sequence-176323-        3253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding N-terminal fusions of 
            TagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: pME-TagBFP2-NS
FEATURES             Location/Qualifiers
     source          1..3253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..60)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(214..802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(294..313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(949..1758)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1672..1691
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1750..1769
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1871..1887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1871..1887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1892..1911
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1892..1910
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1928..2027
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      2727..2736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2737..2836)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2852..2869)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2852..2868)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2861..2883)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2932..3018
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3110..3137
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
       61 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      841 gtggaacgac gcgtaactca cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca
      901 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc
      961 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     1021 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     1081 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     1141 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     1201 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     1261 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     1321 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     1381 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     1441 ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     1501 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     1561 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     1621 atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     1681 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgacgtttc
     1741 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     1801 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg
     1861 ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcagc
     1921 tggatggcaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     1981 taagcaatgc tttcttataa tgccaacttt gtacaagaaa gctgggtgat tcaacttgtg
     2041 ccccaattta ctgggcaagt cgcaatatct agccacagca acttcatgct gctctacgta
     2101 agtctcgttg ttggcttcct taatgcgctc cagacggtaa tccacgtagt acacgccggg
     2161 cattttgagg ttctttgcag gttttttgga cctatacgta gttttggcgt tggcaatcag
     2221 gtgactcccc cccacaagct tcagggccat gtcatttctc ccctcgagac ctccgtctgc
     2281 aggatacagc gtctcggtaa aagcctccca gcccagggtt tttttctgca ttacgggtcc
     2341 attagaggta aagtttacgc ctctgatttt aacgttatat atcagacatc cgtcttgcaa
     2401 tgatgtatcc tgagtggctg tcaggacgcc gccatcctcg taggttgtga ctctttccca
     2461 ggtgaagcct tcggggaatg actgcttgaa aaaatcgggg ataccttgtg tgtgatttat
     2521 aaaagtttta cttccataca ggaaagaggt agccaggatg tcgaaagcga aaggaagagg
     2581 tccaccctcc actactttga tcctcatcgt ctgagttccc tcgtacggtt ttccctcgcc
     2641 ttcacttgta cacttgaagt ggtggttatc tactgtaccc tccatgtaca gtttcatatg
     2701 catattctcc tttatcaatt cactgaccat ggtggcagcc tgcttttttg tacaaagttg
     2761 gcattataaa aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa
     2821 ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact
     2881 gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa
     2941 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3001 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga
     3061 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc
     3121 ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa
     3181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3241 gaatcggcca acg
//
