LOCUS       sequence-176324-        3247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding N-terminal fusions of 
            FusionRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: pME-FusionRed-NS
FEATURES             Location/Qualifiers
     source          1..3247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     449..468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     702..719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(878..905)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(997..1083)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1132..1154
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1146..1163
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1147..1163
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1179..1278
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1279..1288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1982..2081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2098..2117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2099..2117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2122..2138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2240..2259)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2251..3060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2318..2337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3207..3247,1..548)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
       61 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      721 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      781 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
      841 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
      901 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
      961 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     1021 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     1081 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     1141 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
     1201 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
     1261 tgtacaaaaa agcaggctgc caccatggtg agcgagctga ttaaggagaa catgcctatg
     1321 aaactgtaca tggaaggaac agttaacaac catcatttca aatgcactag cgagggagag
     1381 ggcaaacctt atgagggtac gcagaccatg cggatcaaag tggtggaagg cggcccactt
     1441 ccattcgctt ttgatatttt ggcgacatct ttcatgtatg gctcacggac gtttatcaaa
     1501 catcctccag gaatccccga ttttttcaag caatcttttc ccgagggctt cacctgggaa
     1561 agagttacta cctatgagga tggtggcgtc cttacagcaa ctcaggacac atctctccag
     1621 gacggttgcc tgatctacaa cgtgaaagtt aggggcgtga atttcccggc gaacggtccg
     1681 gtgatgcaaa agaaaaccct gggatgggag gcttccacag agacaatgta cccagcggat
     1741 ggcggtctgg agggcgcttg tgacatggct ctcaaacttg tcggaggggg tcacctgatc
     1801 tgcaacctgg agactaccta caggtctaag aagccagcaa caaatctgaa gatgccaggt
     1861 gtttataatg ttgaccacag gcttgaaaga attaaagagg cagatgatga gacatacgtg
     1921 gagcagcatg aagtggcggt ggcacggtac tcaaccggag gagctggcga cggagggaag
     1981 cacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2041 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2101 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2161 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2221 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2281 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2341 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2401 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2461 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2521 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2581 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2641 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2701 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2761 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2821 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2881 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2941 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3001 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3061 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3121 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3241 ttgcaaa
//
