LOCUS       sequence-176327-        9951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4566
FEATURES             Location/Qualifiers
     source          1..9951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(57..386)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      71..206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(125..144)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     371..391
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(400..828)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(510..531)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     722..741
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    874..1098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1087..1104
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1178..1258)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(1265..1312)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5478..5497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5479..5497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5542..5745)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5571..5591)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5746..6125
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6178..6426
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6178..6198
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6349..6368
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     6583..6602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6677..6781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6782..6850,6851..7642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7000..7019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7813..8401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8302..8321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8555..8572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8689..8710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8725..8730,8731..8748,8749..8755)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8763..8779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8768..8790
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8787..8803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8787..8803
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8852..8973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8863..8882)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8917..8936
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9147..9941)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9258..9277)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9868..9887
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cctcatcctg tctcttgatc agatccgaaa atggatatac aagctcccgg gagctttttg
       61 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag
      121 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
      181 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
      241 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      301 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      361 gactttccac accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt
      421 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      481 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
      541 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
      601 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
      661 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
      721 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
      781 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc
      841 tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
      901 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
      961 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     1021 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     1081 ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct ctagactcga
     1141 gcggccgcca ctgtgctgga tatctgcaga attcttaggc atagtcgggg acatcatatg
     1201 ggtatgcata atcaggcacg tcgtagggat aagcgtaatc tggaacatcg tatgggtagg
     1261 atcccttttt cttttttgcc tggccggcct ttttcgtggc cgccggcctt ttcctgttct
     1321 gggcgaaatt cagccaggtc tgattgtcga tggccagctt caccttgttc agatcgtcgg
     1381 agttcttcag ctcattcagc agccacaggc ccttcagggc gatgtggtag gcgccgttgg
     1441 catcggcatt ctgaggctgt gtatcgtcgg ccagggcgct attaaagaac acgccctcgt
     1501 cgtttgccac gggggacagg atgaaatcct cgtcagagga ggcgttgctg taccgcaggg
     1561 ccagcagggt tttcagcagg tacatcagag acttgtgaaa ctccttatca ttgttctggc
     1621 agatgtccat gaccaggttg ggctgcttct cgttgatgtg gtggcgggcg aacagggact
     1681 tcagctcatc attcacgttg atgcccttgg cggcgccctt attctggttg gctgtcttat
     1741 cgtacacgta ccgcttgtcg ccgtgggaac agattgtcca gatctggcgg ctattcttgg
     1801 ccttatcggt aaacttggcg tagtcgatgt gaaactcgaa gtaatccttg tcggcattat
     1861 agcagatctt gtcgaacttg ccaaagaagg cctggctctg ggcgatgttc tcgtatcttg
     1921 gcttcagcag atccacaaag cctgtctcag ggtcgatctt agaggtgttc caggcgggca
     1981 cataaaacag gaagccggtc tgcttgccga tgctcttcag atctgtgaaa ttgttggtca
     2041 gctgcagggc attcttgtaa gagccgatct catcgtcggc cttgtccttc agcaccaggt
     2101 ggttcagctt cttgatcagg gcattctcga agttctgata gatctgcttc tccaccttaa
     2161 agcggcccct cttaaagccg aaattcaggt cctccagcac cacgatggcg ttgtacttca
     2221 gcatcagctg gctgatctgg tgcaccacgt ggctcagata gccagacttc agctccttga
     2281 ttgtctcgat ctcgccccat cccacccggg cgttcaggcg ctcgatctcc ctcttatcca
     2341 ggatcttgtg gtaaggtgtg gtcatctgtg tgccattggc agaggctgtg gtgatgtcgt
     2401 tcagggaaca ctgctccagg atctcgccct tgctattgat cacggtcagg tacagcaggt
     2461 gtctctcgcc ccgggcgatg ccgatcacgt tcacctcgtc atactgctgg atagactggt
     2521 tcaccttctt attgaactcc ttgattgtca tgccctgcac gccaaagttc atggtgattg
     2581 gcacgtgcag catgaacttg tcctgtgtgt acctcttatc cttgatgatg tcgtacacga
     2641 actgtctctt cttaggatta tcgggattct tgttctccag cacctcgccg gccctgtgga
     2701 ttgttgtctc gttcatgtcc agggaggcct ttctgtagaa gatctgggcc tcgccattca
     2761 gcttatagat aggatcggcc aggttgtcct cagaaaacag ggccttgaag tacagggtgt
     2821 gcagattggg cttgccgtgg gcctttgggg aaaagtcctt gttgtagatc tggaacagat
     2881 acagctggcc ctgctccacc agctcatcga tgtagtcggc attgatgttg cagaaggaga
     2941 tcttataatt cagtctccgt gtctcgatct cggtaaacag ctcgttcaca tccacgtaac
     3001 agccgatgtc ctgcagcttc ttggaaaact cgaagctctc ctcccactcc tcgtgcttgc
     3061 acatagactc gtaatagtac cgcaccagat ccttcttaaa cttggggtga ttgttgtaga
     3121 aattctcctt cttgcgatta tcgttggagt cgatcttctt gtagatatta tactgatcca
     3181 ccaggtcctg gcttgggtta tacctcttga actcgccgat aaagaagtcc tccatctcca
     3241 gcttaggctt gctagaaaag atgccgttga tcttatcgaa caggtccttc tcgctgattg
     3301 gcacctctct gcccttctta tccttcttaa acagctggat gtcgccgatg gcgccctcga
     3361 acttcttatc gtacttaggg tggatcttca gacactccag gataaaccga tacagcttca
     3421 ggcgctcatc gtcggatctc tgccggccct tgtccttgat ctccaccagc tccttagaag
     3481 ggtggtagtt gatggcgatg gcctccttgg aaaagaacac cttggggaac tgcttcctca
     3541 cctccaggta cttatagatc atcttctgat agatgctctt gcctgtatta ggggcgttat
     3601 caaacacctt cttgtgggcc ttgtccagca gggccagata gtagcagccg tccttctgca
     3661 ggatcacgcc gaaattatcc ttctccttgt tcaggtccca gccattcagc agtgttggat
     3721 tgccaaagtt cagcttgtac ttctcggtgg agaaaggctt ctggctcagg taatctctca
     3781 ccttgttata cagggtgggg atcttggcca gctcgtcgta cagcacgcca aactcgccat
     3841 agaagttgcc atcctgattg tccagtgtgg tctttgtggt cagcagcttg gcgaagtggg
     3901 ccacattcag ggcgttatcc agcagctcct tcagctgcct cagctgtgtc atctcaggat
     3961 tcttgccgga cttgatcttt ggcagggctc tctctcctgc agggcgctcc ctctccagaa
     4021 agcccttgat ggtgctgtga ttgttgtgga tcttctggat ggggttgtcc actccggcca
     4081 ggccgtgctt gaagtactgt cccagcttgc ctgcctgcac gctctcatcg tcgtgccttg
     4141 cggtatagtg ctcgatggcc tgctccagag aggccaggga gtgcacgccc ttgatgaact
     4201 tatccttctc ctttgtcagc ttggccttgg cattgtcggt cttggccttg gcaaaccgct
     4261 cgttgaactc tggattcacc acatccacat agtatccgtc cagcacgcgt cccagcagtg
     4321 caaagtcgcc gaaggcctgc ttggacagct cattcaggtt cttgtgctcc acgtagatgc
     4381 catccttctg gtgatcgtca aagccgtcga acaggctctg caccttggcg aacacgtcgg
     4441 cgtagtggcg atagaactcg ttcacggcct ggcacatctc gctatcgtcg gcaaacttag
     4501 agggcaggaa ggacacgctc atgccgtcgg acaggatctg cttgtgcagt ggcctcagct
     4561 tggcgattct ctcgctcttg tggcagtgct ggttgtggtg agaattgatc agctcgttga
     4621 tgccctggat cttaggagag cctgcctctc cggagattcc tcccagcagt gtattgtagg
     4681 cggtgatgcc ctcctgtgtc agcagcttgt gatagccatc caggtggctg gccagagaca
     4741 cgtccaggcc gctggcggtc agctcgttga tgatctgatc gtacagggca gagtgcttct
     4801 gcttgattgt ggtcaggatc tgcagattgt cgataaaccg gggcaggttc tcgtggatca
     4861 ggcggtaggc gatggcggtg tgcttatcct cgtcagaata catattcttc cggttatcgt
     4921 gaaagcctgt gaaataggtg gaaaacttct cgaagtgggc caggtgggcc agctttgggg
     4981 agctctctcc ctcctgtgcg atcacgaact tggccagatc gcccagctcc ttgccgtcct
     5041 taaacagctt ggcgccgaac agcctgtcgt agccggcctt atacttgccg ccattgccga
     5101 tgggcttcac gatctccttt ctcagcacgg cctgcagatc cttcagctgc ttctgcagct
     5161 catcgtcctt tgggttcttc cgaaacttca ggtacacgtc atagaactcg gccagcttgg
     5221 tcagcttcac ctcgcccatc atatcggcga tgaagtcgcg gtggtaatcg tccaggatca
     5281 ctttcacctt ctggtgcata tcggccattg tctcgtcctg agacaggaag ttcttggcgt
     5341 ggatgtgctc cagggtccta tcgatgggct tcagctcaaa tctcactgtc ttggacagtg
     5401 gatacaggtg ggtaaagtcc tggaacagca tggtggcggt accaagctta agtttaaacg
     5461 ctagccagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
     5521 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
     5581 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     5641 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     5701 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     5761 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     5821 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     5881 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     5941 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     6001 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     6061 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     6121 atgtcaacgc gccaactagc atatcgatat ggattcggat ctaaggtcgg gcaggaagag
     6181 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     6241 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     6301 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     6361 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     6421 aacaccgaaa tttctactgt ttgtagatgg agacgttcga acgtctcctt ttttgtacgc
     6481 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     6541 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     6601 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
     6661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6781 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6841 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6901 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6961 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7021 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7081 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7141 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7201 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7261 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7321 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7381 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7441 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7501 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7561 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7621 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7681 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7921 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8461 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8521 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8581 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8701 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8761 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     8821 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     8881 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     8941 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     9001 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     9061 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     9121 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     9181 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     9241 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     9301 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     9361 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     9421 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     9481 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     9541 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     9601 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     9661 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     9721 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     9781 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     9841 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     9901 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga t
//
