LOCUS       sequence-176334-        9246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: pKS7107
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..1181
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          250..1172
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1129..1149
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1187..1196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1199..1219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1220..4462
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="AAFKPNSINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVRVF
                     ERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIKS
                     LPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVAG
                     NAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEFG
                     NPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTK
                     LNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDN
                     AEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLK
                     DRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNTE
                     EKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRKE
                     IEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLGR
                     LNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEFK
                     ARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKRV
                     FASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAFD
                     GKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAEK
                     LSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDL
                     EKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKT
                     GVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQL
                     IDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEG
                     IGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     CDS             4463..4489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4496..4522
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     primer_bind     complement(4538..4555)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4544..4768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4933..6046
                     /label=mini-oriP
                     /note="internally truncated variant of the Epstein-Barr 
                     virus oriP replication origin (Tanaka et al., 1999)"
     primer_bind     complement(6091..6107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6091..6107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6104..6126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6115..6131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6139..6145,6146..6163,6164..6169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6184..6205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6322..6339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6493..7081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6573..6592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7252..8043,8044..8112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7875..7894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8113..8217)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(8308..8556)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8594..8612
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(8631..8649)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        complement(8691..8783)
                     /label=Nm tracrRNA
                     /note="trans-activating CRISPR RNA for the Neisseria 
                     meningitidis CRISPR/Cas9 system (Hou et al., 2013)"
     promoter        complement(8783..9031)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(9096..9115)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tttgcgctgc ttcgcggagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
       61 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      121 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      181 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      241 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
      301 ccttgcgtgc cttgaattac ttccacctgg ctccagtacg tgattcttga tcccgagctg
      361 gagccagggg cgggccttgc gctttaggag ccccttcgcc tcgtgcttga gttgaggcct
      421 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      481 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      541 ggcaagatag tcttgtaaat gcgggccagg atctgcacac tggtatttcg gtttttgggg
      601 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      661 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      721 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      781 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tccagggggc tcaaaatgga
      841 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa ggggcctttc
      901 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
      961 attagttctg gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1021 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1081 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1141 cagtggttca aagttttttt cttccatttc aggtgtcgtg aacaccgcca ccatggtgcc
     1201 taagaagaag agaaaggtgg ctgccttcaa acctaattca atcaactaca tcctcggcct
     1261 cgatatcggc atcgcatccg tcggctgggc gatggtagaa attgacgaag aagaaaaccc
     1321 catccgcctg attgatttgg gcgtgcgcgt atttgagcgt gccgaagtac cgaaaacagg
     1381 cgactccctt gccatggcaa ggcgtttggc gcgcagtgtt cgccgcctga cccgccgtcg
     1441 cgcccaccgc ctgcttcgga cccgccgcct attgaaacgc gaaggcgtat tacaagccgc
     1501 caattttgac gaaaacggct tgattaaatc cttaccgaat acaccatggc aacttcgcgc
     1561 agccgcatta gaccgcaaac tgacgccttt agagtggtcg gcagtcttgt tgcatttaat
     1621 caaacatcgc ggctatttat cgcaacggaa aaacgagggc gaaactgccg ataaggagct
     1681 tggcgctttg cttaaaggcg tagccggcaa tgcccatgcc ttacagacag gcgatttccg
     1741 cacaccggcc gaattggctt taaataaatt tgagaaagaa agcggccata tccgcaatca
     1801 gcgcagcgat tattcgcata cgttcagccg caaagattta caggcggagc tgattttgct
     1861 gtttgaaaaa caaaaagaat ttggcaatcc gcatgtttca ggcggcctta aagaaggtat
     1921 tgaaacccta ctgatgacgc aacgccctgc cctgtccggc gatgccgttc aaaaaatgtt
     1981 ggggcattgc accttcgaac cggcagagcc gaaagccgct aaaaacacct acacagccga
     2041 acgtttcatc tggctgacca agctgaacaa cctgcgtatt ttagagcaag gcagcgagcg
     2101 gccattgacc gataccgaac gcgccacgct tatggacgag ccatacagaa aatccaaact
     2161 gacttacgca caagcccgta agctgctggg tttagaagat accgcctttt tcaaaggctt
     2221 gcgctatggt aaagacaatg ccgaagcctc aacattgatg gaaatgaagg cctaccatgc
     2281 catcagccgt gcactggaaa aagaaggatt gaaagacaaa aaatccccat taaacctttc
     2341 tcccgaatta caagacgaaa tcggcacggc attctccctg ttcaaaaccg atgaagacat
     2401 tacaggccgt ctgaaagacc gtatacagcc cgaaatctta gaagcgctgt tgaaacacat
     2461 cagcttcgat aagttcgtcc aaatttcctt gaaagcattg cgccgaattg tgcctctaat
     2521 ggaacaaggc aaacgttacg atgaagcctg cgccgaaatc tacggagacc attacggcaa
     2581 gaagaatacg gaagaaaaga tttatctgcc gccgattccc gccgacgaaa tccgcaaccc
     2641 cgtcgtcttg cgcgccttat ctcaagcacg taaggtcatt aacggcgtgg tacgccgtta
     2701 cggctcccca gctcgtatcc atattgaaac tgcaagggaa gtaggtaaat cgtttaaaga
     2761 ccgcaaagaa attgagaaac gccaagaaga aaaccgcaaa gaccgggaaa aagccgccgc
     2821 caaattccga gagtatttcc ccaattttgt cggagaaccc aaatccaaag atattctgaa
     2881 actgcgcctg tacgagcaac aacacggcaa atgcctgtat tcgggcaaag aaatcaactt
     2941 aggccgtctg aacgaaaaag gctatgtcga aatcgaccat gccctgccgt tctcgcgcac
     3001 atgggacgac agtttcaaca ataaagtact ggtattgggc agcgaaaacc aaaacaaagg
     3061 caatcaaacc ccttacgaat acttcaacgg caaagacaac agccgcgaat ggcaggaatt
     3121 taaagcgcgt gtcgaaacca gccgtttccc gcgcagtaaa aaacaacgga ttctgctgca
     3181 aaaattcgat gaagacggct ttaaagaacg caatctgaac gacacgcgct acgtcaaccg
     3241 tttcctgtgt caatttgttg ccgaccgtat gcggctgaca ggtaaaggca agaaacgtgt
     3301 ctttgcatcc aacggacaaa ttaccaatct gttgcgcggc ttttggggat tgcgcaaagt
     3361 gcgtgcggaa aacgaccgcc atcacgcctt ggacgccgtc gtcgttgcct gctcgaccgt
     3421 tgccatgcag cagaaaatta cccgttttgt acgctataaa gagatgaacg cgtttgacgg
     3481 taaaaccata gacaaagaaa caggagaagt gctgcatcaa aaaacacact tcccacaacc
     3541 ttgggaattt ttcgcacaag aagtcatgat tcgcgtcttc ggcaaaccgg acggcaaacc
     3601 cgaattcgaa gaagccgata ccctagaaaa actgcgcacg ttgcttgccg aaaaattatc
     3661 atctcgcccc gaagccgtac acgaatacgt tacgccactg tttgtttcac gtgcgcccaa
     3721 tcggaagatg agcgggcaag ggcatatgga gaccgtcaaa tccgccaaac gactggacga
     3781 aggcgtcagc gtgttgcgcg taccgctgac acagttaaaa ctgaaagact tggaaaaaat
     3841 ggtcaatcgg gagcgcgaac ctaagctata cgaagcactg aaagcacggc tggaagcaca
     3901 taaagacgat cctgccaaag cctttgccga gccgttttac aaatacgata aagcaggcaa
     3961 ccgcacccaa caggtaaaag ccgtacgcgt agagcaagta cagaaaaccg gcgtatgggt
     4021 gcgcaaccat aacggtattg ccgacaacgc aaccatggtg cgcgtagatg tgtttgagaa
     4081 aggcgacaag tattatctgg taccgattta cagttggcag gtagcgaaag ggattttgcc
     4141 ggatagggct gttgtacaag gaaaagatga agaagattgg caacttattg atgatagttt
     4201 caactttaaa ttctcattac accctaatga tttagtcgag gttataacaa aaaaagctag
     4261 aatgtttggt tactttgcca gctgccatcg aggcacaggt aatatcaata tacgcattca
     4321 tgatcttgat cataaaattg gcaaaaatgg aatactggaa ggtatcggcg tcaaaaccgc
     4381 cctttcattc caaaaatacc aaattgacga actgggcaaa gaaatcagac catgccgtct
     4441 gaaaaaacgc ccgcctgtcc gttacccata cgatgttcca gattacgctg cagctccagc
     4501 agcgaagaaa aagaagctgg attaactcgc tgatcagcct cgactgtgcc ttctagttgc
     4561 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4621 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4681 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4741 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     4801 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     4861 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccctg
     4921 gccggggtcc cgcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
     4981 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
     5041 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     5101 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     5161 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     5221 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
     5281 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
     5341 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
     5401 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     5461 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
     5521 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
     5581 aggatagcat atgctatcca gatatttggg tagtatatgc tatccatggc aacattagcc
     5641 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
     5701 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
     5761 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
     5821 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
     5881 aaaaccgcag ggcggcgtgt gggggctgac gcgatcgctg ttccttagga cccttttact
     5941 aaccctaatt cgatagcata tgcttcccgt tgggtaacat atgctattga attagggtta
     6001 gtctggatag tatatactac tacccgggaa gcatatgcta cccgtttagg gtttaccgtc
     6061 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6121 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6181 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6241 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6301 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6901 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7021 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7081 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7141 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7201 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7261 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7321 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7381 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7441 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7501 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7561 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7621 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7681 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7741 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7801 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7861 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7921 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7981 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8041 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8101 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8161 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8221 atttccccga aaagtgccac ctgacgtcgt ttaaacaaaa aaacgaaatg agaaagggag
     8281 ctacaacgga gacgggatcc cgtctccggt gtttcgtcct ttccacaaga tatataaagc
     8341 caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa
     8401 ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa
     8461 tatctttgtg tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat
     8521 atgcaaatat gaaggaatca tgggaaatag gccctcttcc tgcccgacct tagatccgaa
     8581 tccatatcga tatgctagtt attgctcagc ggtggcagca gccaactcag cctatagtga
     8641 gtcgtattaa tttcgcggga tgtttaaacg cggccgcaaa aaaaccgaaa taaacgatgc
     8701 cccttaaagc agaagcttta aggggcagag cgttgcggca catcttttca gacggcctta
     8761 ttgtagcaac ggttctcatt tcggtgtttc gtcctttcca caagatatat aaagccaaga
     8821 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
     8881 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
     8941 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
     9001 aatatgaagg aatcatggga aataggccct cttcctgccc gaccttgacg tcgacggatc
     9061 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9121 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9181 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9241 aggcgt
//
