LOCUS       sequence-176337-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GF-ori-FP plasmid for GFxFP assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: pWN10042
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     173..192
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     rep_origin      457..1045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     946..965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
       61 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      121 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      181 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      241 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
      301 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      361 cttcagggtc agcttgccga tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1081 cttttgctgg ccttttgctc acatgagatc atcgaattcg tcgacctgca ggctagcgga
     1141 tcctcagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     1201 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatctt
//
