LOCUS       sequence-176339-        3662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab9a cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     348..365
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    482..503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(518..523,524..541,542..548)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    556..572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     561..583
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     580..596
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     580..596
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        614..632
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     614..631
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1373..1382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1377..1386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1416..1435)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1417..1435)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1442..1459)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1442..1458)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1451..1473)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1599..2054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1686..1705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1896..1917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2132..2236
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2237..2305,2306..3097)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2455..2474)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3268..3662,1..194)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
       61 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      241 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      301 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      361 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      421 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      481 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      541 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      601 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg
      661 ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga ttagctagtt
      721 gcctctcctc cattaacagc actgtgtagc gctgcgcggt ttcctgtgca gattcacagt
      781 gtctgtagga atcagatgct cgtgtctgtc ctccagagac agcactcttc tcaccgcctc
      841 ctcgaacgca accgcgacat tagtggcatc ttttgcactg gtctcgaaat atgggtatcc
      901 accgctctcc atgcaccact cctgcgcttc ttcactgctc acctgacgct ccgaaacgtc
      961 cagtttattt cccagaacca caaacgggaa gctttcgggc tccttgacgt cggcgtaata
     1021 aatgaactct tttttccagt tgaccaggtt gtggaagctc tggctgtcat ccacactgaa
     1081 ggtcagcagg cagcagtcgg agccgcggta gaaaggcgtg cggaggctac ggaagcgctc
     1141 ctgaccggct gtatcccaga tctgcagggt cacagtacgg ccgtccacct ccaagtcttt
     1201 attgaggaac tccacaccga tggtgtggaa gagatgagca tcaaacttgt tggtgacata
     1261 tcggttcatg agcgagcttt tgcccacgcc gccatcaccc agcagaatca ctttcaggag
     1321 agatgattta gatgacatgg ttgtaatcct cgtcctgaat cgaattcccg cggccgccat
     1381 ggcggccggg agcatgcgac gtcgggccca attcgcccta tagtgagtcg tattacaatt
     1441 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1501 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1561 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1621 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1681 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1741 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1801 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1861 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1921 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1981 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2041 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2101 cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2161 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2221 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2281 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2341 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2401 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2461 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2521 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2581 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2641 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2701 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2761 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2821 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2881 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2941 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3001 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3061 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3121 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3181 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3241 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3301 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3361 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3421 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3481 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3541 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3601 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3661 at
//
