LOCUS       sequence-176340-       10485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kamicas9 lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..10485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..104)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        89..418
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      269..404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1552..1617
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1747..1767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2192..2326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2229..2248)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2283..2302
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2494..3127
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3174..3299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3792..4025
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4210..4254
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4521..4638
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4674..4698
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4724..4938
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     4859..4878
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        4965..5040
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        5345..5420
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5457..5481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5504..5520)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5505..5521)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        5537..6036
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5572..5595)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5969..5988
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6051..6060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6057..6767
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6202..6220)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6470..6489
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6863..7451
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6916..6936)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7334..7345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7540..7773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7798..7816)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7799..7816)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8106..8125)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8339..8358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8458..8480
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8518..8536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8604..8708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8709..8777,8778..9569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8927..8946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9740..10328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10229..10248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(10482..10485,1..14)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
       61 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      421 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
      481 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
      541 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
      601 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
      661 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
      721 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
      781 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
      841 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
      901 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
      961 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     1021 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     1081 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     1141 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     1201 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     1261 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     1321 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     1381 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     1441 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     1501 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     1561 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     1621 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     1681 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     1741 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     1801 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     1861 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     1921 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     1981 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     2041 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     2101 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2161 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2221 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2281 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     2341 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     2401 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     2461 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     2521 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     2581 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     2641 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     2701 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     2761 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     2821 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     2881 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     2941 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3001 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3061 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3121 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     3181 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     3241 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     3301 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     3361 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     3421 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     3481 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     3541 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     3601 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     3661 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     3721 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     3781 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     3841 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     3901 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     3961 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     4021 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     4081 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     4141 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     4201 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     4261 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     4321 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     4381 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     4441 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     4501 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4561 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4621 acaaaaattc aaaattttat cgctagtgga tcccccgggc tgcaggaatt atcacaagtt
     4681 tgtacaaaaa agcaggcttc atcgatcaat tgggatccaa ttcgaacgct gacgtcatca
     4741 acccgctcca aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg
     4801 ccctggcagg aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc
     4861 gctatgtgtt ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata
     4921 agttctgtat gagaccacag atctgaccct ggaaaagctg atgagtttta gagctagaaa
     4981 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     5041 ttttttagat cggatccaag cttccatggg cggccgctct agactgcagt atttagcatg
     5101 ccccacccat ctgcaaggca ttctggatag tgtcaaaaca gccggaaatc aagtccgttt
     5161 atctcaaact ttagcatttt gggaataaat gatatttgct atgctggtta aattagattt
     5221 tagttaaatt tcctgctgaa gctctagtac gataagtaac ttgacctaag tgtaaagttg
     5281 agatttcctt caggtttata tagcttgtgc gccgcctggg taccaatgga gtacttcttg
     5341 tccagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     5401 aagtggcacc gagtcggtgc ttttttggta ctctagactc gagactagta ggcctgaccc
     5461 agctttcttg tacaaagtgg tgataattcg atatcaagct tatcgatacc gtcgacctcg
     5521 acgatggtcg agtaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     5581 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     5641 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     5701 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5761 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5821 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     5881 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     5941 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     6001 gcgctgttct cctcttcctc atctccgggc ctttcgacct ctaggatccc gccaccatgg
     6061 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     6121 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6181 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     6241 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     6301 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6361 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     6421 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     6481 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     6541 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     6601 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     6661 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     6721 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaagc ggccgcgtcg
     6781 agagatgggg gaggctaact gagctagctt agtcatgcac cgggctgcag gaattcgcgg
     6841 ccgcactcga gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     7261 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7381 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7441 cgcctccccg catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     7501 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7561 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7621 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7681 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7741 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     7801 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     7861 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     7921 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     7981 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     8041 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     8101 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     8161 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     8221 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     8281 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     8341 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8401 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8461 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     8521 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8941 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9241 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9601 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9661 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9721 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9841 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9901 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9961 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10021 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10081 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10141 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10201 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10261 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10321 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10381 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10441 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcg
//
