LOCUS       sequence-176344-        3641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab10 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(107..695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(187..206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(866..1657,1658..1726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1489..1508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1727..1831)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1909..2364)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2046..2067)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2258..2277
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2490..2512
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2504..2521
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2505..2521
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2528..2547
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2528..2546
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2577..2586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2581..2590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3310..3328)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3311..3328)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3346..3362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3346..3362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3359..3381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3370..3386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3394..3400,3401..3418,3419..3424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3439..3460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3577..3594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
       61 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      121 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      181 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      241 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      301 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      361 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      421 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      481 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      541 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      601 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      721 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      781 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      841 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      901 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
      961 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1021 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1081 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1141 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1201 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1261 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1321 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1381 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1441 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1501 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1561 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1621 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1681 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1741 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1801 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg
     1861 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     1921 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1981 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     2041 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2101 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     2161 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2221 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     2281 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     2341 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     2401 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     2461 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     2521 tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg ctcccggccg
     2581 ccatggcggc cgcgggaatt cgattctgtg agtgtgtgtt caactgcagc acttggtctt
     2641 ccagcctgtg actcctcctc cagtgctgat gtccacgttt tcactgttag gctcttttac
     2701 gggtgtcttt ttgagaatgt cttctgctaa cgtgaggaac gccttctcga tgttgatgtt
     2761 ggcttttgca ctcgtctcga agaagcgaat cccgtgttct tttgcgatct gttccccctt
     2821 ggcttttggt acgacacgtt tgtcctccat gtcacacttg ttgcctagca gcatcctctc
     2881 cacatcctca ttagcgtgct catcaatgtt tctgagccat ttgctgatgt tttcgaagct
     2941 ctttgcattg gtgatgtcat atacgagcat gattcccatt gcccctctgt agtaagaggt
     3001 agtgatggtg tgaaaccgtt cctgccctgc tgtatcccat atctgtagct ttatcttctt
     3061 tccttgcaat tcaactgttt tgatcttaaa atcgattcct atagtggaga taaaggtggt
     3121 gttgaaggcg tcatcggaga atcggaacag cacgcaggtc tttcccaccc cagaatcgcc
     3181 aatcaagagc agtttaaaga gcagatcata ggtcttcttc gccatctgaa atcactagtg
     3241 aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag
     3301 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
     3361 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3421 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3481 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3541 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag g
//
