LOCUS       sequence-176347-        2683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCAG vector for expressing V5-NLGN2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: pCAG-V5-NLGN2
FEATURES             Location/Qualifiers
     source          1..2683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             167..208
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cattttggca aagaattccg ccaccatggc acttcccaga tgcatgtggc caaattatgt
       61 ttggagagct atgatggcat gtgtggtcca caggggatcc ggtgccccat tgactctctg
      121 cttgttggga tgtttgctac agacttttca cgtactctct caaaagggca agcccatccc
      181 caaccccctg ctgggcctgg acagcaccca acggggagga gggggtcccg gcggcggcgc
      241 cccgggcggc ccaggcctgg gcctcggcag cctcggggag gagcgcttcc ccgtggtgaa
      301 cacagcctac gggcgagtgc gcggtgtgcg gcgcgagctc aacaacgaga tcctgggccc
      361 ggtcgtgcag ttcttgggcg tgccctacgc cacgccgccc ttgggcgccc gccgcttcca
      421 gccgcctgag gcacctgcct cgtggcccgg cgtgcgcaac gccaccaccc tgccgcccgc
      481 ctgcccgcag aacctgcacg gggccctgcc ggccatcatg ctgcctgtgt ggttcaccga
      541 caacttggag gcggccgcca cctacgtgca gaaccagagc gaggactgcc tgtacctcaa
      601 cctctacgtg cccactgagg acggtccgct cacaaaaaaa cgtgacgagg cgacgctcaa
      661 tccgccagac acagatatcc gggactctgg gaagaaaccg gtcatgctgt ttctacacgg
      721 cggctcctac atggaaggca ccgggaacat gtttgacggc tcagtcctgg ctgcctatgg
      781 caatgtcatc gtagccacac tcaactaccg tcttggggtg ctcggttttc tcagcactgg
      841 tgaccaggct gcaaaaggca actatgggct cctggaccag atccaggccc tgcgctggct
      901 cagtgaaaac attgcccact ttggaggtga ccctgaacgc atcactatct ttgggtctgg
      961 tgcaggggcc tcctgtgtca acttgctgat cctttcccac cactcagaag gactgttcca
     1021 gaaggccatt gctcaaagtg gtactgccat ttccagctgg tctgtcaact accagccgct
     1081 caagtacacg cggctgctgg cggccaaagt gggctgtgac cgagaagaca gcactgaagc
     1141 tgtggagtgt ctgcgccgga agtcttcccg ggagctagtg gaccaggatg tacagcctgc
     1201 ccgctaccac attgcctttg ggcctgtggt ggacggcgac gtagtccctg atgaccccga
     1261 gatcctcatg caacaggggg aattcctcaa ctacgacatg ctcattggtg tcaaccaggg
     1321 agagggtctc aagttcgtgg aggactctgc agagagtgag gacggtgtgt ctgccagcgc
     1381 ctttgacttc accgtctcca actttgtgga caacttgtac ggttacccag aaggcaagga
     1441 cgtgcttcga gagaccatca agttcatgta cacggactgg gctgacaggg acaatggcga
     1501 gatgcggcgg aagaccctgc tggcgctctt taccgaccac cagtgggtcg ccccggctgt
     1561 ggccaccgcc aagctgcatg ccgactacca gtcccccgtc tacttttaca ctttctacca
     1621 ccactgccag gcagagggcc ggccagagtg ggcagacgca gcgcacgggg acgagctgcc
     1681 ctacgtcttt ggtgtcccca tggtgggcgc cactgacctc ttcccctgca acttctccaa
     1741 gaacgatgtc atgctcagcg cagtagtcat gacctactgg accaacttcg ccaagactgg
     1801 tgaccccaac cagcctgtgc cacaggacac caagttcatc cacaccaagc ccaaccgctt
     1861 tgaagaggta gtgtggagca agttcaacag caaggaaaag cagtatctgc acataggctt
     1921 gaaaccacgc gtgcgcgaca actaccgtgc caacaaggtg gccttctggc tggagctcgt
     1981 gccccacctg cacaacctgc acacagagct cttcaccacc accactcgcc tgcctcccta
     2041 tgccacacgc tggccacctc gcacacctgg tcctggcact tccggcacac gccgtcctcc
     2101 cccacctgcc actctgccac ctgagtctga tattgaccta ggcccaaggg cctatgaccg
     2161 cttccccggt gactcgaggg actactccac ggagctaagc gtgactgtgg cagtgggtgc
     2221 ctccctcctc ttcctcaaca tccttgcctt tgccgccctc tattacaagc gggaccggcg
     2281 ccaggagctg cggtgccgga ggcttagccc accaggaggc tcaggctcag gtgtgcctgg
     2341 tgggggcccc ctgcttccca ctgctggccg tgagctaccc ccggaggagg agctagtatc
     2401 gctgcagctg aagcggggtg gtggtgttgg ggcggaccct gctgaggccc tgcgccctgc
     2461 ctgtccaccc gactataccc tggccttgcg ccgggcaccg gacgatgtgc ctctcttggc
     2521 ccccggggcc ctaaccctgc tgcctagtgg cctggggccc ccgcccccgc ccccaccccc
     2581 ttctctccat ccctttgggc ccttcccacc cccaccccct actgctacca gccacaacaa
     2641 cacgctaccc catccccact ccaccactcg ggtatagaag ctt
//
