LOCUS       sequence-176350-        6083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCAG vector for expressing V5-NLGN2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: pCAG-V5-NLGN2
FEATURES             Location/Qualifiers
     source          1..6083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     326..348
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     410..429
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      474..483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             621..662
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3135..3154)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3200..3255
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3254..3273)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3616..3632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3616..3632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3629..3651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3640..3656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3664..3670,3671..3688,3689..3694))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3709..3730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3789..3984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3835..3970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3990..4124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4027..4046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4081..4100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4192..4209)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4363..4951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4443..4462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5122..5913,5914..5982))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5745..5764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5983..6074)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
       61 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
      121 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
      181 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
      241 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
      301 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
      361 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      421 gttattgtgc tgtctcatca ttttggcaaa gaattctgca gtcgacggta cccgccacca
      481 tggcacttcc cagatgcatg tggccaaatt atgtttggag agctatgatg gcatgtgtgg
      541 tccacagggg atccggtgcc ccattgactc tctgcttgtt gggatgtttg ctacagactt
      601 ttcacgtact ctctcaaaag ggcaagccca tccccaaccc cctgctgggc ctggacagca
      661 cccaacgggg aggagggggt cccggcggcg gcgccccggg cggcccaggc ctgggcctcg
      721 gcagcctcgg ggaggagcgc ttccccgtgg tgaacacagc ctacgggcga gtgcgcggtg
      781 tgcggcgcga gctcaacaac gagatcctgg gcccggtcgt gcagttcttg ggcgtgccct
      841 acgccacgcc gcccttgggc gcccgccgct tccagccgcc tgaggcacct gcctcgtggc
      901 ccggcgtgcg caacgccacc accctgccgc ccgcctgccc gcagaacctg cacggggccc
      961 tgccggccat catgctgcct gtgtggttca ccgacaactt ggaggcggcc gccacctacg
     1021 tgcagaacca gagcgaggac tgcctgtacc tcaacctcta cgtgcccact gaggacggtc
     1081 cgctcacaaa aaaacgtgac gaggcgacgc tcaatccgcc agacacagat atccgggact
     1141 ctgggaagaa accggtcatg ctgtttctac acggcggctc ctacatggaa ggcaccggga
     1201 acatgtttga cggctcagtc ctggctgcct atggcaatgt catcgtagcc acactcaact
     1261 accgtcttgg ggtgctcggt tttctcagca ctggtgacca ggctgcaaaa ggcaactatg
     1321 ggctcctgga ccagatccag gccctgcgct ggctcagtga aaacattgcc cactttggag
     1381 gtgaccctga acgcatcact atctttgggt ctggtgcagg ggcctcctgt gtcaacttgc
     1441 tgatcctttc ccaccactca gaaggactgt tccagaaggc cattgctcaa agtggtactg
     1501 ccatttccag ctggtctgtc aactaccagc cgctcaagta cacgcggctg ctggcggcca
     1561 aagtgggctg tgaccgagaa gacagcactg aagctgtgga gtgtctgcgc cggaagtctt
     1621 cccgggagct agtggaccag gatgtacagc ctgcccgcta ccacattgcc tttgggcctg
     1681 tggtggacgg cgacgtagtc cctgatgacc ccgagatcct catgcaacag ggggaattcc
     1741 tcaactacga catgctcatt ggtgtcaacc agggagaggg tctcaagttc gtggaggact
     1801 ctgcagagag tgaggacggt gtgtctgcca gcgcctttga cttcaccgtc tccaactttg
     1861 tggacaactt gtacggttac ccagaaggca aggacgtgct tcgagagacc atcaagttca
     1921 tgtacacgga ctgggctgac agggacaatg gcgagatgcg gcggaagacc ctgctggcgc
     1981 tctttaccga ccaccagtgg gtcgccccgg ctgtggccac cgccaagctg catgccgact
     2041 accagtcccc cgtctacttt tacactttct accaccactg ccaggcagag ggccggccag
     2101 agtgggcaga cgcagcgcac ggggacgagc tgccctacgt ctttggtgtc cccatggtgg
     2161 gcgccactga cctcttcccc tgcaacttct ccaagaacga tgtcatgctc agcgcagtag
     2221 tcatgaccta ctggaccaac ttcgccaaga ctggtgaccc caaccagcct gtgccacagg
     2281 acaccaagtt catccacacc aagcccaacc gctttgaaga ggtagtgtgg agcaagttca
     2341 acagcaagga aaagcagtat ctgcacatag gcttgaaacc acgcgtgcgc gacaactacc
     2401 gtgccaacaa ggtggccttc tggctggagc tcgtgcccca cctgcacaac ctgcacacag
     2461 agctcttcac caccaccact cgcctgcctc cctatgccac acgctggcca cctcgcacac
     2521 ctggtcctgg cacttccggc acacgccgtc ctcccccacc tgccactctg ccacctgagt
     2581 ctgatattga cctaggccca agggcctatg accgcttccc cggtgactcg agggactact
     2641 ccacggagct aagcgtgact gtggcagtgg gtgcctccct cctcttcctc aacatccttg
     2701 cctttgccgc cctctattac aagcgggacc ggcgccagga gctgcggtgc cggaggctta
     2761 gcccaccagg aggctcaggc tcaggtgtgc ctggtggggg ccccctgctt cccactgctg
     2821 gccgtgagct acccccggag gaggagctag tatcgctgca gctgaagcgg ggtggtggtg
     2881 ttggggcgga ccctgctgag gccctgcgcc ctgcctgtcc acccgactat accctggcct
     2941 tgcgccgggc accggacgat gtgcctctct tggcccccgg ggccctaacc ctgctgccta
     3001 gtggcctggg gcccccgccc ccgcccccac ccccttctct ccatcccttt gggcccttcc
     3061 cacccccacc ccctactgct accagccaca acaacacgct accccatccc cactccacca
     3121 ctcgggtata gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     3181 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     3241 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     3301 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     3361 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     3421 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     3481 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     3541 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     3601 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3781 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3961 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     4021 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4081 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     4141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4801 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5161 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5401 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5521 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6061 gaaaaataaa caaatagggg ttc
//
