LOCUS       sequence-176351-       12336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-MDGA2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-MDGA2
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    422..539
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(837..881)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1066..1299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1792..1917
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1964..2144
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2162..2360)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2187..2207)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2362..2741
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2913..2932
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3007..3111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3112..3180,3181..3972)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3330..3349)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4143..4731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4632..4651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4885..4902
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5019..5040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5055..5060,5061..5078,5079..5085)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5093..5109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5098..5120
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5117..5133
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5117..5133
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5182..5303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5193..5212)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5247..5266
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5433..5807)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(5826..5873)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5921..6250)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5935..6070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5989..6008)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6235..6255
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6264..6692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6374..6395)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6586..6605
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6738..6962
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6951..6968
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6994..7174
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     7679..7695
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7680..7696
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7699..8287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7805..7816
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8214..8234
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11130..11171)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      12215..12224
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(12232..12336,1..343))
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ctcgcgcctc gcgtggtgcg gtccggctgg gccggcggcg gcgcggacgc gaccaaggtg
       61 gccgggaagg ggagtttgcg ggggaccggc gagtgacgtc agcgcgcctt cagtgctgag
      121 gcggcggtgg cgcgcgccgc caggcggggg cgaaggcact gtccgcggtg ctgaagctgg
      181 cagtgcgcac gcgcctcgcc gcatcctgtt tcccctcccc ctctctgata ggggatgcgc
      241 aatttgggga atgggggttg ggtgcttgtc cagtgggtcg gggtcggtcg tcaggtaggc
      301 acccccaccc cgcctcatcc tggtcctaaa acccacttgc actcatacgc agggccctct
      361 gcagatgcat ggcggtatta attaaccaaa ctggatctct gctgtccctg taataaaccc
      421 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
      481 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
      541 tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga tccgttcact
      601 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
      661 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
      721 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
      781 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
      841 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
      901 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
      961 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     1021 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     1081 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     1141 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     1201 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     1261 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     1321 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     1381 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     1441 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     1501 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     1561 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     1621 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     1681 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     1741 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     1801 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     1861 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     1921 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     1981 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     2041 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     2101 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaacgc
     2161 gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     2221 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     2281 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     2341 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     2401 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     2461 attgacgtca atagggggcg tgcttggcat atgatacact tgatgtactg ccaagtgggc
     2521 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     2581 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     2641 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     2701 cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg
     2761 tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt
     2821 caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac
     2881 acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     2941 gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga
     3001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3121 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3181 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3241 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3301 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3361 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3421 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3481 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3541 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3601 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3661 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3721 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3781 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3841 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3901 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3961 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4261 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4501 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4741 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4801 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4861 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4921 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4981 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5041 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5101 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     5161 gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5221 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     5281 ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc
     5341 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     5401 gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc
     5461 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
     5521 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
     5581 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
     5641 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
     5701 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
     5761 acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt ttagttcctc
     5821 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
     5881 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
     5941 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg
     6001 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     6061 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
     6121 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
     6181 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
     6241 actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt
     6301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     6481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     6541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     6601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     6661 cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt
     6721 tctttccgcc tcagaagcca tagagcccgc cgcatcccca gcatgcctgc tattgtcttc
     6781 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     6841 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     6901 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     6961 agtcgaggct gatcagcggg tttaaacggg ccctgctaga gattttccac actgactaaa
     7021 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     7081 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     7141 cccaggctca gatctggtct aaccagagag acccagtaca gtccggatgc agctctcggg
     7201 ccatgtgatg aaatgctagg cggctgtcaa acctccactc taatacttct ctctccgggt
     7261 catccatccc atgcaggctc acagggtgta acaagcgggt gttctctcct tcattggctt
     7321 cttctacctt ctcttgctca actggtacta gcttgtagca ccatccaaag gtcagtggat
     7381 atctgatccc tggccctggt gtgtagttct gccaatcagg gaagtagcct tgtgtgtggt
     7441 agatccacag atcaaggata tcttgtcttc gttgggagtg aattagccct tccagtcccc
     7501 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     7561 gtacctgagg tgtgactgga aaacccacct cctcctcctc ttgtgcttct agccaggcac
     7621 aatcagcatt ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc
     7681 gaggtcgacg gtatcgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
     7741 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     7801 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     7861 cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag
     7921 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg
     7981 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     8041 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     8101 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     8161 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     8221 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     8281 gttgattatc gataagcttg atatcgaatt ccctgcaggg gcgcgcctca ccttcgaggg
     8341 cttaagatcg agatgaggat gataactgga aaaagccata tatgaactaa gatcccaaca
     8401 gcaccatcca cggaattctt agttggtagg tcttgttttg cacattctcc ttctgcaatt
     8461 gacacatcgt caatggcgat gtcaccttct atcccaggac cccgaatgcc ttcaaaaatt
     8521 aactgaaatg aagtaattgg atatatatta acatgagctt cattccatcg ttgtcctttg
     8581 ttcccactcg aagaccatag aggattctct attgtcgtct gccctttcaa acgtagatat
     8641 acatttaaaa cacctatatg ttgcccatac atgtgataga agaaactgaa acaatatgca
     8701 ctattggtag gtccatatgg atttttggga gctatgctga atacagggct gagaagtcga
     8761 gccttttctc cttctagtct gggtcgcgat gtctcgatgt acatataaaa accttctttg
     8821 gaaccactcc gatctgcatt gggacctgtg ttaggggtgt attttgtatt tcttgttgca
     8881 gtactttgct ttgtccagtc aaagttatct gtatcatctt gggtgaataa gcatatatta
     8941 ccatcttcaa atccacagtg aaattctctc aaatgaggat ttacaggtgc cgtatattta
     9001 attacacgaa ttgttgaatc tccttcacca aatttagtga gaggagtcag tcggacttca
     9061 taggcctctg gttttataag ttcagtcaag ttatatgtaa ttagttctcc cttttgaata
     9121 ttcccattta ttttaatctc ctgttcccac caacgttgct gtccagcctg ccgaataccc
     9181 aaacggtatg cgacaatccg atccactgca tcgggattca tttgtgtcca ctgcagactg
     9241 taagaataca ctcggtgtcg gttctgccac acagggttgt aggtgtcata gtagaactct
     9301 ggggcatagg cttttcctgt cacaagaaag ctgcatctcc ctgctccagc ttcattaatg
     9361 atgctgcagt tataaactcc atagttttca ttggaaagac tctttagtgg gtactcagta
     9421 tactcctgag agtcaaactg accggtccgt aataatttat tgcccagacg ccattcgtaa
     9481 gtcagcacac gtatcggata ggctcttaga accctacagc tcatggtcac acttctgtct
     9541 tgtccttgac ggatttccaa gaatgctggt tccacagctg ggggatactg cacgatgagc
     9601 tgcaccaagg cttctctggg cttcacgttg aatccattgt actgactggt ctggcatctg
     9661 tacatcccag acatttccct tgacacgttc acaatcctga gggtcccatc ataactctcc
     9721 atttgcattg tcccatcagg cattgcaact tctttatctg ctctagacca aaggatgatt
     9781 ggttttggtt tcccagttac ttgacactga agttctattg tgtccccttc tctggtgacc
     9841 aaaggtgatt tttcctgtgg aacagtcaga ttgggtggaa ctgtgctgct ggaaatgtta
     9901 acgtcaatac tgatatcaga tattcctcct cccttcagag aggccacgca tgtgtaagtc
     9961 ccaaagtccg tgaattttaa atcaatgata tccaaatttg ttgttccggg ggacacatca
    10021 gggtcagtct gtgtaataac catccgctca gaacttctta atggacggcc gtttttaaac
    10081 caactgaatg ttaactcttc agagggaacg gcttctactt ggcaagatat tttcacctca
    10141 cgcccaatct ggatgttgtc atctttgtga taaggatcag gagtgatcca aaatcgtcct
    10201 ttttttaatg ctctcacaat gatgttggtg gacttttttg cagggtttcc cacattatta
    10261 ttggcaatgc agctgtaagt cccagcatca tcagaggtga tggcaggtat ggtcaatgtc
    10321 cctccattca aaacaatctt ttcaggcaga gtcccgaagg acctgaccca ggtgagagag
    10381 ggcataggct ctccccctgt tgtaacacac actaacgtta tggcctctcc aggatttaca
    10441 actatagggt catccaccaa gagcttaatt gacggagaag cggttttatt ggaaagtcta
    10501 aaagacacca tcttgtctgg aatattacac acgttcctca ctgaagcaat gcagctgtag
    10561 ttagcatagt cctgaggtcg gagattcttt agctttaaga tctttgtttc cccctgggta
    10621 aaaaagggct cgtaaatctc cactcctttg tctgatcctt gtagcaacac ctcttggcca
    10681 cgtctccagc tgtagcgcac aggggggttg gaattggcca cacatcggag gaacactgtt
    10741 ctctcatagt aaaattgttc tttagcttca cctatacttt gatgaacagt tactacagga
    10801 tcatccaagt agtacacatc cactcggatc gactttattg ctggagaccc caagccattc
    10861 tctgctttac agtaatatcg gcctccttgg tgtctctgaa tgcttgtaat cctcaaagtc
    10921 tcattgaaga cacttgagtc ttggaatctg tcagaggcgc ttcctgctgt tttggtccac
    10981 ctgatctgtg ggcgtggatg tccagtaacc agacaggtca actctagcgt ttctccttcc
    11041 cggatggtat agaccctttc cgagtagcgc tcctcctcta tattacaggc caatcctgag
    11101 tgcacaatcc gaaccgtcgg gggaccggtg gtgctgtcca ggcccagcag ggggttgggg
    11161 atgggcttac cgcccgagcc cgagccagag ttgctgttca cgaccctaca gttcagtctt
    11221 atctgtccct gcgtacccgt caggggcgtt atatttccca tccgatccat agcttccacg
    11281 aaagcattga aaaaggtttg tgtgctatta gcgaagctgc gcacaagagg aatagtgtcg
    11341 gtagcatttg gggaactgaa cagctcctgg tcggactgaa tcaggccctt ctgttcctcc
    11401 agattgacgt aatacttatt gtcgaagatg gttggggtgc gcaggtcaaa gtcaaccagt
    11461 gcagacaagt tgccattgag cggacacagc cctctgagag tctgcaagta tgttgtgttc
    11521 agggtagggt caggcaagcc ggtgttggaa aagttataca gtctgtccat gatgaacctg
    11581 cactgattct tgccgaatgt gtggcctccg gacagagcca ccaaatcact acttcggttc
    11641 aggccgacat tcctgaagga atccttcagc tggggaaggg tgaagaaggg agcaggcagg
    11701 tttgcgtttg ccaagtccag aaaagcctgc agagagtccc tccttcccag ggggaccctc
    11761 cagctggggc caccggccag cgtcacggat tgctgggccg ctatggtcag aaggtctgca
    11821 caactaactg tccgggggca ggcgctctcc acagcggctt tcattctatc gatcacggga
    11881 aaccctctag cactattcgc gttaccgaag gcatcctttt cagtcctgaa agaggtggta
    11941 ttgtcgagga ggatggaagc atcacagccg tttacaaaac aatcgtggaa atgcagccgc
    12001 aaaattgaag cggctatcct cggatcagac cgcaactcgt tcacgatagt atctcgcacg
    12061 atgttgctca cgttaggaca agagttatcg tagaaagttg gagtaagctg gctagcggcg
    12121 ggcagcatct gaaagcaggc ccccagcaga cacaagacca cccccgaggg cctcctcagc
    12181 agccagtata gacagagacc gagcagcagg aaatccatgg tggcgtctag actgcgctct
    12241 caggcacgac acgactcctc cgctgcccac cgcagactga ggcagcgctg agtcgccggc
    12301 gccgcagcgc agatggtcgc gcccgtgccc ccctat
//
