LOCUS       sequence-176352-        4900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression vector for V5-NLGN2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-NLGN2
FEATURES             Location/Qualifiers
     source          1..4900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..490
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      522..531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1614..1655
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4171..4759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4224..4244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4642..4653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4762..4778)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4763..4779)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata ccgccatgca tctgcagagg gccctgcgta tgagtgcaag tgggttttag
       61 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa
      121 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag
      181 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc
      241 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
      301 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
      361 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
      421 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
      481 gagagcgcag tctagaggat ccttcgaaac cggtgctagc cgccaccatg gcacttccca
      541 gatgcatgtg gccaaattat gtttggagag ctatgatggc atgtgtggtc cacaggggat
      601 ccggtgcccc attgactctc tgcttgttgg gatgtttgct acagactttt cacgtactct
      661 ctcaaaagac cggtcagctt actccaactt tctacgataa ctcttgtcct aacgtgagca
      721 acatcgtgcg agatactatc gtgaacgagt tgcggtctga tccgaggata gccgcttcaa
      781 ttttgcggct gcatttccac gattgttttg taaacggctg tgatgcttcc atcctcctcg
      841 acaataccac ctctttcagg actgaaaagg atgccttcgg taacgcgaat agtgctagag
      901 ggtttcccgt gatcgataga atgaaagccg ctgtggagag cgcctgcccc cggacagtta
      961 gttgtgcaga ccttctgacc atagcggccc agcaatccgt gacgctggcc ggtggcccca
     1021 gctggagggt ccccctggga aggagggact ctctgcaggc ttttctggac ttggcaaacg
     1081 caaacctgcc tgctcccttc ttcacccttc cccagctgaa ggattccttc aggaatgtcg
     1141 gcctgaaccg aagtagtgat ttggtggctc tgtccggagg ccacacattc ggcaagaatc
     1201 agtgcaggtt catcatggac agactgtata acttttccaa caccggcttg cctgacccta
     1261 ccctgaacac aacatacttg cagactctca gagggctgtg tccgctcaat ggcaacttgt
     1321 ctgcactggt tgactttgac ctgcgcaccc caaccatctt cgacaataag tattacgtca
     1381 atctggagga acagaagggc ctgattcagt ccgaccagga gctgttcagt tccccaaatg
     1441 ctaccgacac tattcctctt gtgcgcagct tcgctaatag cacacaaacc tttttcaatg
     1501 ctttcgtgga agctatggat cggatgggaa atataacgcc cctgacgggt acgcagggac
     1561 agataagact gaactgtagg gtcgtgaaca gcaactctgg ctcgggctcg ggcggcaagc
     1621 ccatccccaa ccccctgctg ggcctggaca gcacccaatt gcaacgggga ggagggggtc
     1681 ccggcggcgg cgccccgggc ggcccaggcc tgggcctcgg cagcctcggg gaggagcgct
     1741 tccccgtggt gaacacagcc tacgggcgag tgcgcggtgt gcggcgcgag ctcaacaacg
     1801 agatcctggg cccggtcgtg cagttcttgg gcgtgcccta cgccacgccg cccttgggcg
     1861 cccgccgctt ccagccgcct gaggcacctg cctcgtggcc cggcgtgcgc aacgccacca
     1921 ccctgccgcc cgcctgcccg cagaacctgc acggggccct gccggccatc atgctgcctg
     1981 tgtggttcac cgacaacttg gaggcggccg ccacctacgt gcagaaccag agcgaggact
     2041 gcctgtacct caacctctac gtgcccactg aggacggtcc gctcacaaaa aaacgtgacg
     2101 aggcgacgct caatccgcca gacacagata tccgggactc tgggaagaaa ccggtcatgc
     2161 tgtttctaca cggcggctcc tacatggaag gcaccgggaa catgtttgac ggctcagtcc
     2221 tggctgccta tggcaatgtc atcgtagcca cactcaacta ccgtcttggg gtgctcggtt
     2281 ttctcagcac tggtgaccag gctgcaaaag gcaactatgg gctcctggac cagatccagg
     2341 ccctgcgctg gctcagtgaa aacattgccc actttggagg tgaccctgaa cgcatcacta
     2401 tctttgggtc tggtgcaggg gcctcctgtg tcaacttgct gatcctttcc caccactcag
     2461 aaggactgtt ccagaaggcc attgctcaaa gtggtactgc catttccagc tggtctgtca
     2521 actaccagcc gctcaagtac acgcggctgc tggcggccaa agtgggctgt gaccgagaag
     2581 acagcactga agctgtggag tgtctgcgcc ggaagtcttc ccgggagcta gtggaccagg
     2641 atgtacagcc tgcccgctac cacattgcct ttgggcctgt ggtggacggc gacgtagtcc
     2701 ctgatgaccc cgagatcctc atgcaacagg gggaattcct caactacgac atgctcattg
     2761 gtgtcaacca gggagagggt ctcaagttcg tggaggactc tgcagagagt gaggacggtg
     2821 tgtctgccag cgcctttgac ttcaccgtct ccaactttgt ggacaacttg tacgggtacc
     2881 cagaaggcaa ggacgtgctt cgagagacca tcaagttcat gtacacggac tgggctgaca
     2941 gggacaatgg cgagatgcgg cggaagaccc tgctggcgct ctttaccgac caccagtggg
     3001 tcgccccggc tgtggccacc gccaagctgc atgccgacta ccagtccccc gtctactttt
     3061 acactttcta ccaccactgc caggcagagg gccggccaga gtgggcagac gcagcgcacg
     3121 gggacgagct gccctacgtc tttggtgtcc ccatggtggg cgccactgac ctcttcccct
     3181 gcaacttctc caagaacgat gtcatgctca gcgcagtagt catgacctac tggaccaact
     3241 tcgccaagac tggtgacccc aaccagcctg tgccacagga caccaagttc atccacacca
     3301 agcccaaccg ctttgaagag gtagtgtgga gcaagttcaa cagcaaggaa aagcagtatc
     3361 tgcacatagg cttgaaacca cgcgtgcgcg acaactaccg tgccaacaag gtggccttct
     3421 ggctggagct cgtgccccac ctgcacaacc tgcacacaga gctcttcacc accaccactc
     3481 gcctgcctcc ctatgccaca cgctggccac ctcgcacacc tggtcctggc acttccggca
     3541 cacgccgtcc tcccccacct gccactctgc cacctgagtc tgatattgac ctaggcccaa
     3601 gggcctatga ccgcttcccc ggtgactcga gggactactc cacggagcta agcgtgactg
     3661 tggcagtggg tgcctccctc ctcttcctca acatccttgc ctttgccgcc ctctattaca
     3721 agcgggaccg gcgccaggag ctgcggtgcc ggaggcttag cccaccagga ggctcaggct
     3781 caggtgtgcc tggtgggggc cccctgcttc ccactgctgg ccgtgagcta cccccggagg
     3841 aggagctagt atcgctgcag ctgaagcggg gtggtggtgt tggggcggac cctgctgagg
     3901 ccctgcgccc tgcctgtcca cccgactata ccctggcctt gcgccgggca ccggacgatg
     3961 tgcctctctt ggcccccggg gccctaaccc tgctgcctag tggcctgggg cccccgcccc
     4021 cgcccccacc cccttctctc catccctttg ggcccttccc acccccaccc cctactgcta
     4081 ccagccacaa caacacgcta ccccatcccc actccaccac tcgggtatag ggcgcgcccc
     4141 tgcagggaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     4201 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4261 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4321 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4381 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4441 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4501 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4561 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4621 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4681 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4741 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     4801 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     4861 gaggaggagg tgggttttcc agtcacacct caggtacctt
//
