LOCUS       sequence-176431-       10532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of hDicer in Sf9 Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   MacRae IJ, Ma E, Zhou M, Robinson CV, Doudna JA
  TITLE     In vitro reconstitution of the human RISC-loading complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):512-7. doi: 
            10.1073/pnas.0710869105. Epub 2008 Jan 4.
  PUBMED    18178619
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0710869105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-01-15- 15"; volume: "105"; issue: "2"; pages: "512-7"
COMMENT     Sequence Label: pFastBac-hDicer
FEATURES             Location/Qualifiers
     source          1..10532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  178..402
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(536..553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(707..1295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(787..806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1466..2257,2258..2326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2089..2108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2327..2431)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2457..2911)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2594..2615)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2805..2824
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  3095..3260
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    3289..3423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3313..3332)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3367..3386
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9343..9363)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(9385..9402)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(9469..9560)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(9527..9544)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(9912..9917,9918..9934,9935..9940)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             join(10110..10532,1..111)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc
       61 atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt
      121 caagccgaga tcggcttccc ggccgcggag ttgttcggta aattgtcaca acgccgcgaa
      181 tatagtcttt accatgccct tggccacgcc cctctttaat acgacgggca atttgcactt
      241 cagaaaatga agagtttgct ttagccataa caaaagtcca gtatgctttt tcacagcata
      301 actggactga tttcagttta caactattct gtctagttta agactttatt gtcatagttt
      361 agatctattt tgttcagttt aagactttat tgtccgccca cacccgctta cgcagggcat
      421 ccatttatta ctcaaccgta accgattttg ccaggttacg cggctggtct atgcggtgtg
      481 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
      541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     2461 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2521 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2581 gttgagtgtt gttccagttt ggaacaagag tccactatta agaacgtgga ctccaacgtc
     2641 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2701 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2761 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2821 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2881 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcaaataagc
     2941 gttgatattc agtcaattac aaacattaat aacgaagaga tgacagaaaa attttcattc
     3001 tgtgacagag aaaaagtagc cgaagatgac ggtttgtcac atggagttgg caggatgttt
     3061 gattaaaaac ataacaggaa gaaaaatgcc ccgctgtggg cggacaaaat agttgggaac
     3121 tgggaggggt ggaaatggag tttttaagga ttatttaggg aagagtgaca aaatagatgg
     3181 gaactgggtg tagcgtcgta agctaatacg aaaattaaaa atgacaaaat agtttggaac
     3241 tagatttcac ttatctggtt cggatctcct aggctcaagc agtgatcaga tccagacatg
     3301 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3361 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3421 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3481 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt
     3541 ctcgacaagc ttggtaccgc atgcctcgag tcagctattg ggaacctgag gttgattagc
     3601 tttgaggctt cggagggctc ttcttgctgc tgcagatttg gcaatcctgt aacttcgacc
     3661 aacaccttta aatttcccct ttcctactac ttccacagtg actctgacct tcccgtcgta
     3721 agttctctca gccgggctaa atttggcagt ttctggttcc atttcaagca attctcgcac
     3781 aggggaacgg ggtacatttg cagaaaactt ttctattagt ggccgcatca tgggatagta
     3841 cacctgccag actgtctcca gtgacatccc actatccatg taaatggcac cagcaagcga
     3901 ctcaaaaata tcccccatgg cctttggaac ttcaatatcc tcttctttct cttcatcctc
     3961 ctcagatctc ctaagctcag aatccattcc ttgcatttca ttcttctcaa gctgaaactg
     4021 cacaaagtca tcaatgacat ggaagagctc aggagagaca gctttgaagt acttgtggta
     4081 gtcgtacttt acagccagcg atgcaaagat ggtgttgttg accagggcag accgcaggtc
     4141 tgtcaggacc cccggggagt gctgccgcgg gtcttcataa aggtgcttgg ttatgaggta
     4201 gtccaaaatc gcatctccca ggaattctaa gcgctggtaa caatcagtga tagtattgta
     4261 gtggtaggag gcatgtgtaa aagcctggag aaggtaagcc ttattcttga atctgtagtt
     4321 gattttcttt tcaaaatttt caaaccccga tataaggtga ttcagtgttt tatctgcatc
     4381 tggatgatca aacatacatc ttggtggaat cttcaaacaa ccatattccg agtctttcaa
     4441 tacagaagag cgtgaactgg ccacagaagc agcagcacag ctcactgaaa ggttcttttg
     4501 ttggctgttg aaattctccc gagtagggca cagggccttt tcccgatcag tccttttaat
     4561 taccgggagc accttcagcc ccagtgaaca gaggaaaagc tgagcagccc tctccccaca
     4621 gctggttaaa tagcagccca gcagggcttc cacacagtcc gctatgcttt tgtcagcaat
     4681 acactgctca gtgtgcaagt cgtaagaaat ggactgcttt cccgtgtcaa caccacagtt
     4741 ttcttctgat ggattccaga accccaccac aaagtcatct tcttcaacag ctttgctagg
     4801 atccagatag cacattgcat cccaagagct gtagtcaaaa tcctcaaaat ctgatgaaaa
     4861 tggcatacta cctaaggagg attttttggg cattttccat tcatatgcag aatcagtggt
     4921 tgaaaaagga gaaagagaga ttttctttgc aaaagctcct gaccccatta acatattatc
     4981 tataaatctg atatgttcct gatcatactc caggaaatca tcttcatagt cagcctcttc
     5041 cttcggagcc ctccacatca ggctctcctc ctcctcatcc tcctcctcgt aatcctcatc
     5101 cagtttgcca ttcgccagca tgcagtcttt tgtcatttca tctttttccc atttatctgt
     5161 gttgcttttg tcttgattta ctacataacc aggaggaagc caattcacag ggggatcaaa
     5221 tattgacacc accatgcggc tgggtagtcc cttctttttt ccaaggcgat acagattaca
     5281 gttgctgacc tttttgcttc tcatatatga aaggcggccc tcatgcgcat cagggtaagt
     5341 gcaaaataga tatgtggtga tggcatgctt taaaaaggag tcgccaagca tttcaagccg
     5401 ctccaggtta aatccatcac tagcgtttga cagagtcaaa gcctgaagaa taagtccagg
     5461 attggggcca agagtccttg aggagtaccc aatagaaggg ctctgctcag aatccatcct
     5521 gcccttgagc acttgaatag tgtctgtcgt accaggcatt acggccatca caggacttcc
     5581 atctgaggta gatttgttag catttccatc aaggtattta ttactcagga gagtacattc
     5641 atcgctgggc tggggctggt tctcgtaact gtataaattc tgaatggaat atgaggtagt
     5701 tggttgcacg ggtatttcct gcttgtagta atttagctga tttccttggc aaaagtctct
     5761 gttagctaaa tcatagctgc cattggcgag attttgattg taagaaagac cattaattgc
     5821 tgtaagatct gctgaaactt caacgtggag cttaccaggg gactcgctga gcaacgttct
     5881 gcagttcaca gacatttggt catgattttc tagagaggag gttctattag caccttgatg
     5941 tgcagcattt tcagggacaa ttgtgctgtg cttacagtaa ttatcatttt cagctgaaga
     6001 ggagttagaa attgagatga aagatttgct gtcaatagat tttttccacc cgaagtctaa
     6061 gttagggtat ctaaaatccg caggaagtga tctgactccc acgccagcat cgctggcagt
     6121 ctgggctctt agctcctctg cagtcaaaag gcagtgaagg cgataaagta tgctggggag
     6181 acaaacagct tttctccaca gtgatgctgg aattggatgt atagcacaga gttctggaac
     6241 cagtatctgt ttattctgca gactttccca tttggctttc ctcttctcag cactgcttaa
     6301 aggaagcgct ttccccttct gattcaaatg tcgaggtgtc aaaagattaa gtcttgaaga
     6361 tgtgtggtcc acatccagca gtggctggtt gagattggtt aggtcaaggt tgtactttgt
     6421 tttataatat tctgcaaaag tttcatactc aggggaagga aatttactga gtggggtaag
     6481 atcagtgtac acatcagcta cataaaatcg atgaggctga tcaaaattgc gatatcttgg
     6541 aatgataacg gcatcttggt aatcttctaa tttaaaaaca aagggtgttt cttttgtata
     6601 ctttgtactg ggaatgccta tgcgagcttc agacttctca atatcttcca tgaatttaaa
     6661 gtcaatatcc aaagtgctgg agtcattaac aacattaaga ggtagaacac agtatgctga
     6721 atcagcgtct gtaggtttaa attctagtgc aggtttttca agccgaagaa tatgtgagaa
     6781 tatatactgg tgaagtcttg taatcaactc aagcatttgt agagacaaca tgaaaccaga
     6841 cttcttcaac tcaatggata tggtaacctc tccagagcgt gtgtacacag gaaagtgtgg
     6901 aatctgaggt atgggtttgg ccgtcagtat tccaaagcat cttgtggtat cttcaggagg
     6961 atagagcttc cgccttctaa agttgagttc atcaggtaaa ggtgtagtta aaaccattcc
     7021 tatcacatac aggtaacagg gctgatcagg tctgggataa ctatccctca aacactctgg
     7081 aattgctttt gggtagcact gccttcgttt cgtggaacct ggtcttcctg gaacactggt
     7141 ctcttcttca tcatgcaaat caagctcctc ttcatattta acagtctctt tcccaactgg
     7201 catcaaatgg tcatccagtt cgccaatttt gtgcagtttc tcacagcaaa tgagagctac
     7261 aactctttca gccaatcgta cacagctcat tggtggacca acaatggagg ctcgaagagg
     7321 tgagttaatt ggcagataaa gagttgaata aaatgtacca tcaggcaact ctcgggttct
     7381 gcatttagga gctagatgag taaacggatc acttggtaat ctagcacagt atctattgat
     7441 gtgtccaatg gccgtgttga ttgtgactcg tggaccacca tcgtcaggcc tcaacacata
     7501 tggtgggaaa acgtcatcat catccatgac aggatcaatg tcagtctcac cagtatcaac
     7561 cgacttggaa cacttgtttc tcaagatctt ttcaatagct ttgtaggttt taaggtcttc
     7621 ttcaaaactt tttattttgt ctgtatccgc taacattata taattagaga tgggtgccct
     7681 tgctcttcct ttagattgaa cataggatcg atattctgtg ggcaaatcaa aacgaaccac
     7741 caagttgcat tttggtatat caacaccctc ttctacaata cttgttgcaa taagcaggtt
     7801 ggtctcatgt gctcgaaatt tcctaagtac ctcttcctgt tttctgaatt ctgcttccat
     7861 ctgtttgttg cgaggctgat tcttcccaat gccatgtcca gttatgaaat tgctactgat
     7921 ataagccagc tctggatctt gtttgccagc ttcctttatc aatctgttta agacaactgc
     7981 tgtgtatctt ctttccacaa aaataattcc gcacaaaatg ttggtaaaag gagaaggaaa
     8041 atttgtctct ggcttctctt tttcttcaat ttcttcatcc tcatcatcat cctcagaatc
     8101 actccatgac acataattat cctgatttct attattatac cactcaacgc tttcaaactg
     8161 ctgtcgctca tatggtttat atttgcgtaa gatttcgagc agtttgatta ctttaggagt
     8221 tacaaatttc aggtcaagtg aggcaggtga gaagtgctct tcacatagtg catgtatttt
     8281 ccttaggaaa gtgtctgtaa acaataaaaa tttcctgtgc agctcctctt gctcatgttt
     8341 gatgtatttc tgtagttctc ttaccatcat tccagctact ttatctgcac accagggtcc
     8401 cagaactacc aatacggcac gacagtctga tagtatctgt ttcgaaatta aagtagaatc
     8461 tctttctttt gaatgtacag atatattaca atcattgata aaattaagtg cttcttctaa
     8521 ttccatcagc agtctttcat aaagcccact tctgtcagta aatggtccac aatccaccac
     8581 aatctcacat ggctgagaag tatacctgtc taagaccacc aggtcagttg cagtttcagc
     8641 attactctta agaattttct ctagtttctg aatcttttct tccaattcct ctggatcaca
     8701 tttcccattt aaaatggaag cagttagtcc caaaatgcga ggacatgatg gacaattttc
     8761 acagagcttc ataatttctc gataggggtg gtctaggatt gcaagatgac actcatcaaa
     8821 caccaaaagg ttaatgtctg acagtgataa gtaaccattt ttcaaaacat tcaaggcgac
     8881 atagcaagtc ataatgagaa cctggtgctt agtaaactct tggttccatc tctcttttgt
     8941 ccaagatgca tttacttcta ggtttgagta ttccccaacc ttgagatctg aatgagttct
     9001 gacagctgac acttgttgag caacctggtt tgcagagttg accaagaaca ccgtcctttt
     9061 tccatttctg ctgaagtctc ccctgatctg ataggacagc tctttagtga gtagtactgc
     9121 aataaatgtc ttccctgagc cagtgtttaa acagacgatg gtattatgat ccagagctgc
     9181 ttcaagcagt tcaacctgat attttcttgg cgtataaatg ttatcatgaa ttgcttcttg
     9241 ttgccatggc agtccaaaga aaggacccat tggtgaggaa gcaggggtca tgagctgcag
     9301 gcctgccatg ctgaggggtt gcaaagcagg gcttttcatg gcgccctgaa aatacaggtt
     9361 ttcggtcgtt gggatatcgt aatcgtgatg gtgatggtga tggtagtacg acatggtttc
     9421 ggaccgagat ccgcgcccga tggtgggacg gtatgaataa tccggaatat ttataggttt
     9481 ttttattaca aaactgttac gaaaacagta aaatacttat ttatttgcga gatggttatc
     9541 attttaatta tctccatgat ctattaatat tccggagtat acgtagccaa ccactagaac
     9601 tatagctaga gtcctgggcg aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa
     9661 ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt cttccgatct
     9721 cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc aaggccgcca
     9781 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg
     9841 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     9901 cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc
     9961 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
    10021 ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc
    10081 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
    10141 agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt cgcacatgta
    10201 ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc ggtcgtgagt
    10261 tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc gggaacttgc
    10321 tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt gttggcgctc
    10381 tcgcggctta cgttctgccc aagtttgagc agccgcgtag tgagatctat atctatgatc
    10441 tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc aatctcctca
    10501 agcatgaggc caacgcgctt ggtgcttatg tg
//
