LOCUS       sequence-176434-        3726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ELK1 encoding amino acid 307-428. Cloning sites BamHI and Xba I. 
            Size 365bp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ
  TITLE     Integration of MAP kinase signal transduction pathways at the serum 
            response element.
  JOURNAL   Science. 1995 Jul 21. 269(5222):403-7.
  PUBMED    7618106
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1995 Jul 21. 269(5222):403-7."
COMMENT     Sequence Label: pSG424 ELKC
FEATURES             Location/Qualifiers
     source          1..3726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..511
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    1024..1158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1061..1080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1115..1134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1221..1240)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1505..1522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1676..2264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1756..1775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2435..3226,3227..3295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3058..3077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3296..3400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3455..3475)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tgagctattc cagaagtagt gaagaggctt ttttggagga gatctaagct tgaagcaagc
       61 ctcctgaaag atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa
      121 aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga
      181 gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt
      241 ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct
      301 tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt
      361 tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga
      421 tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag
      481 taacaaaggt caaagacagt tgactgtatc gccggaattc ccggggatcc cccagccgca
      541 gaagggccgg aagccccggg acctagagct tccactcagc ccgagcctgc taggtgggcc
      601 gggacccgaa cggaccccag gatcgggaag tggctccggc ctccaggctc cggggccggc
      661 gctgacccca tccctgcttc ctacgcatac attgaccccg gtgctgctga cacccagctc
      721 gctgcctcct agcattcact tctggagcac cctgagtccc attgcgcccc gtagcccggc
      781 caagctctcc ttccagtttc catccagtgg cagcgcccag gtgcacatcc cttctatcag
      841 cgtggatggc ctctcgaccc ccgtggtgct ctccccaggg ccccagaagc catgatctag
      901 aattctagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
      961 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1021 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1081 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1141 tcttatcatg tctggatcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     1201 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     1261 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     1321 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     1381 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata
     1441 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     1501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2101 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     2761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     3061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3421 cacctgacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3481 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3541 aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3601 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3721 ggcctc
//
