LOCUS       sequence-176438-        4851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the 
            CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene
            (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Seth A, Gonzalez FA, Gupta S, Raden DL, Davis RJ
  TITLE     Signal transduction within the nucleus by mitogen-activated protein 
            kinase.
  JOURNAL   J Biol Chem. 1992 Dec 5;267(34):24796-804.
  PUBMED    1332967
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1992-12-5"; volume: "267(34)"; pages: "24796-804"
COMMENT     Sequence Label: pG5ElbLuc
FEATURES             Location/Qualifiers
     source          1..4851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        89..107
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     89..106
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    141..235
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             326..1978
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(392..410)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2449..2583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2486..2505)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2540..2559
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2593..2612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2594..2612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2699..2716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2870..3458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2950..2969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3629..4420,4421..4489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4252..4271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4490..4594)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4662..4680
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     join(4840..4851,1..8)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat
       61 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaact cgagcagctg
      121 aagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
      181 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
      241 actctagagg gtatataatg gatccccggg taccgagctc gaattagctt atgcatgcgg
      301 ccgcatctag agggcccgga tccaaatgga agacgccaaa aacataaaga aaggcccggc
      361 gccattctat cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
      421 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
      481 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
      541 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
      601 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
      661 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
      721 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
      781 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
      841 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
      901 tgcactgata atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca
      961 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     1021 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     1081 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     1141 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     1201 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     1261 tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca
     1321 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     1381 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     1441 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     1501 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     1561 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     1621 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc
     1681 tgaattggaa tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     1741 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     1801 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     1861 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     1921 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa
     1981 tgtaactgta ttcagcgatg acgaaattct tagctattgt aatcctccga ggcaaggact
     2041 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     2101 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     2161 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     2221 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     2281 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     2341 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2401 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     2581 atctgccggt ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4621 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4681 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     4741 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     4801 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag c
//
