LOCUS       sequence-176443-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI 
            cloning sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC
  TITLE     Synergistic drug combinations for cancer identified in a CRISPR 
            screen for pairwise genetic interactions.
  JOURNAL   Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834.
  PUBMED    28319085
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834."
COMMENT     Sequence Label: pMCB306
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        467..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     620..640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             683..863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    910..1035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1532..1765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1950..1994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2292..2409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2662..2695)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2699..2719
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2964..3175
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3176..4114
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4071..4091
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4135..4731
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4135..4154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4631..4651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4741..4794
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4801..4810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4807..4809,4810..4812,4813..5526)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4852..4873)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5113..5132)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5460..5481
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5549..5582)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5638..6226
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5691..5711)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6109..6120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6229..6245)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6230..6246)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6755..6935
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6997..7585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7077..7096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7756..8547,8548..8616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8379..8398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8617..8721)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8796..8815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
       61 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      661 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     1381 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1441 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1501 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1561 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1621 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1681 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1741 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1801 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1861 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1921 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1981 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2041 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2101 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2161 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2221 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2281 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2341 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2401 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
     2461 ctctagagat ccgacgcgcc atctctaggc ccgcgccggc cccctcgcac ggacttgtgg
     2521 gagaagctcg gctactcccc tgccccggtt aatttgcata taatatttcc tagtaactat
     2581 agaggcttaa tgtgcgataa aagacagata atctgttctt tttaatacta gctacatttt
     2641 acatgatagg cttggatttc tataacttcg tatagcatac attatacgaa gttataaaca
     2701 gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt gttttgagac tataagtatc
     2761 ccttggagaa ccaccttgtt ggaccaggat gggcaccacc cgtttaagag ctaagctgga
     2821 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2881 tcggtgcttt ttttctcgag tactaggatc cattaggcgg ccgcgtggat aaccgtatta
     2941 ccgccatgca tgtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3001 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     3061 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3121 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     3181 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     3241 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     3301 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     3361 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     3421 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     3481 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     3541 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     3601 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     3661 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     3721 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     3781 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     3841 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     3901 cgattagttc tcgcgctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     3961 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     4021 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     4081 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgagctagcc caccatgacc
     4141 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     4201 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     4261 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4321 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4381 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4441 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4501 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     4561 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     4621 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     4681 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatcggga
     4741 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaccggtc
     4801 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4861 ctggacggcg acgtaaacgg ccacaagttc agcgtgtctg gcgagggcga gggcgatgcc
     4921 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4981 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5041 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5101 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5161 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5221 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5281 aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5341 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5401 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5461 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5521 aagtaataag aattcgtcga gggacctaat aacttcgtat agcatacatt atacgaagtt
     5581 atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct tatcgataat
     5641 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5701 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5761 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5821 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5881 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5941 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6001 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6061 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6121 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6181 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6241 cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6301 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     6361 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6421 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6481 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     6541 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     6601 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     6661 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     6721 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6901 cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa
     6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     8761 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8821 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     8881 atttaagcta caacaaggca a
//
