LOCUS       sequence-176446-        8837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hU6 sgRNA cloning vector for CDKO system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC
  TITLE     Synergistic drug combinations for cancer identified in a CRISPR 
            screen for pairwise genetic interactions.
  JOURNAL   Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834.
  PUBMED    28319085
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834."
COMMENT     Sequence Label: pKHH030
FEATURES             Location/Qualifiers
     source          1..8837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             735..915
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    962..1087
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1584..1817
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2002..2046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2344..2461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2529..2777
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2529..2549
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2700..2719
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2953..3164
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3165..4103
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4060..4080
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4124..4720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4124..4143)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4620..4640
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4730..4783
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4790..4799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4796..4798,4799..4801,4802..5515)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4841..4862)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5102..5121)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5449..5470
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5538..5571)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5627..6215
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5680..5700)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6098..6109)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6218..6234)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6219..6235)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6744..6924
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6986..7574)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7066..7085)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7744..8535,8536..8604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8367..8386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8605..8709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8784..8803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
       61 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      121 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      661 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
      721 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      901 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      961 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     1021 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     1081 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     1141 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1201 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1261 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1321 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1381 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     1441 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1501 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1561 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1621 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1681 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1741 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1801 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1861 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1921 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1981 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     2041 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     2101 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     2161 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2221 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     2281 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
     2341 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2401 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2461 tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc cgctctcgag
     2521 gcggccgcga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     2581 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2641 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2701 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2761 ggaaaggacg aaacaccggt gtcttcgaga agacctgttt aagagctaag ctggaaacag
     2821 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2881 gctttttttc ctaggggatc cattatctag agcgtggata accgtattac cgccatgcat
     2941 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     3001 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     3061 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     3121 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     3181 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     3241 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     3301 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3361 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3421 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     3481 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     3541 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     3601 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3661 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3721 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     3781 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     3841 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3901 cgcgcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3961 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     4021 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     4081 aaagtttttt tcttccattt caggtgtcgt gagctagccc accatgaccg agtacaagcc
     4141 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     4201 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     4261 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4321 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4381 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4441 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4501 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4561 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4621 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4681 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc ggatcgggag agggcagagg
     4741 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaccggtcg ccaccatggt
     4801 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4861 cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa
     4921 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4981 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5041 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5101 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5161 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5221 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5281 caaggcgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5341 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5401 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5461 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaataaga
     5521 attcgtcgag ggacctaata acttcgtata gcatacatta tacgaagtta tacatgttta
     5581 agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga
     5641 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5701 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5761 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5821 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5881 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5941 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6001 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6061 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6121 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgacttcgcc ttcgccctca
     6181 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     6241 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6301 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6361 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6421 gactggaagg gctaattcac tcccaacgaa gtcaagatat ccttgatctg tggatctacc
     6481 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6541 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     6601 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     6661 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     6721 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6781 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6841 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6901 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     6961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7021 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7081 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7141 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7201 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7261 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7321 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7381 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7441 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7501 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7561 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cgtggaacga
     7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8761 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8821 ctgctccctg cttgtgt
//
