LOCUS       sequence-176448-        3888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab40c cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      145..600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(232..251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     442..463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        678..782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(783..851,852..1643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1001..1020)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1814..2402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2303..2322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2556..2573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2690..2711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2726..2731,2732..2749,2750..2756)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2764..2780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2769..2791
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2788..2804
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2788..2804
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2822..2840
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2822..2839
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3807..3816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3811..3820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3850..3869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3851..3869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(join(3876..3888,1..5))
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(join(3876..3888,1..4))
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(3885..3888,1..19))
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
       61 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      121 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      181 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      241 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      301 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      361 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      421 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      481 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      541 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      601 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
      661 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      721 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      781 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      841 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      901 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      961 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1021 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1081 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1141 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1201 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1261 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1321 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1381 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1441 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1501 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1561 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1621 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1681 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1741 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1801 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1861 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1921 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1981 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2041 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2101 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2161 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2221 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2281 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2341 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2401 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2461 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2521 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2581 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2641 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2701 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2761 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     2821 tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg agctctccca
     2881 tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt gtgcctggag gctaggagat
     2941 cttacagttg ttcctggtgc agttctgtgg tggactttgt ggcggccgga tggatttgga
     3001 gcgtttaaga ctgttacctt tgctgcctcc ggcggcattg gccagcgaat acgagcggcc
     3061 gtgcatcatg acagcattca tgccgttcgc catggagaat gacttcaaat ggctcttgat
     3121 ggccacagga agtggcagct tatcgattag gtgcacaggg gtgcaggaaa caatggcacg
     3181 gcagcacaag tcctgcagac tgaatactct gttgggcctc cagaacttct ccatcccatg
     3241 cctcatcagg acgatgcgcg agagctctgt aaaggactcg atgacgttga agttgcagag
     3301 aggactcact tcaaaaaatg tcatgccatt cttttctgcg tatgcccgtg cctgttctgt
     3361 gggaacctgt cgcttgaacg ccaggtgtag ccggttaccc actagaatac ggggcactcc
     3421 aggtgcatgc tcatcaatct ccctgatcca gcggtctatc ccatcaaatg accagcggtt
     3481 ggtgatgtca tacaccaaca agatcccctg cgctcccctg gagtacgacc tgaaaatggt
     3541 gcaaaacctg ccttgtcccg aagtgtccca aagctctagt ttaactctcc gtccatccaa
     3601 caggatcgtg gttgtcttgt agtcgattcc gctgctgtag gcatatggag actcagcaga
     3661 tccgtcttga aggctatcca ggatctctcc cttgcccacg tcgctgtctc cgaccaggag
     3721 gaacttgagc aggtagtcgt agctcttcac agggcttccc tgggtgccca tgactactca
     3781 catcagagag caatcgaatt cccgcggccg ccatggcggc cgggagcatg cgacgtcggg
     3841 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtt
//
