LOCUS       sequence-176450-       11076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human EPHB4 and GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos 
            A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, 
            Lloreta J, Sancho E, Batlle E
  TITLE     EphB-ephrin-B interactions suppress colorectal cancer progression by
            compartmentalizing tumor cells.
  JOURNAL   Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.
  PUBMED    17906625
REFERENCE   2  (bases 1 to 11076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2007-11"; volume: "39(11)"; pages: "1376-83. Epub 
            2007 Sep 30"
COMMENT     Sequence Label: FUW-ubiquitin-EphB4-SV40-GFP (B4G)
FEATURES             Location/Qualifiers
     source          1..11076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        94..473
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..673
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     628..648
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             691..871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    918..1043
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1536..1769
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1954..1998
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2296..2413
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2472..3683
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3520..3539
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        7633..7990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7633..7824
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7656..7676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7841..7976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7903..7922
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8038..8085
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8104..8478
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8608..8729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8645..8664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8699..8718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8778..8794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8778..8794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8791..8813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8802..8818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8826..8832,8833..8850,8851..8856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8871..8892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9009..9026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9180..9768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9260..9279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9939..10730,10731..10799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10562..10581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10800..10904)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10979..10998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
       61 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      121 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      181 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      421 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      481 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      541 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      601 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      661 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      841 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      901 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      961 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1021 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1081 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1141 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1201 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1261 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1321 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1381 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1441 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1501 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1561 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1621 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1681 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1741 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1801 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1861 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1921 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1981 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     2041 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2101 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2161 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2221 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2281 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2401 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaggc
     2461 cggcctgcag cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2521 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2581 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2641 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2701 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2761 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2821 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2881 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2941 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     3001 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     3061 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     3121 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3181 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3241 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3301 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3361 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3421 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3481 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3541 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3601 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3661 cgtttttggc ttttttgtta gacaggatcc tacccgagtg aggcggcgcc atggagctcc
     3721 gggtgctgct ctgctgggct tcgttggccg cagctttgga agagaccctg ctgaacacaa
     3781 aattggaaac tgctgatctg aagtgggtga cattccctca ggtggacggg cagtgggagg
     3841 aactgagcgg cctggatgag gaacagcaca gcgtgcgcac ctacgaagtg tgtgacgtgc
     3901 agcgtgcccc gggccaggcc cactggcttc gcacaggttg ggtcccacgg cggggcgccg
     3961 tccacgtgta cgccacgctg cgcttcacca tgctcgagtg cctgtccctg cctcgggctg
     4021 ggcgctcctg caaggagacc ttcaccgtct tctactatga gagcgatgcg gacacggcca
     4081 cggccctcac gccagcctgg atggagaacc cctacatcaa ggtggacacg gtggccgcgg
     4141 agcatctcac ccggaagcgc cctggggccg aggccaccgg gaaggtgaat gtcaggacgc
     4201 tgcgtctggg accgctcagc aaggctggct tctacctggc cttccaggac cagggtgcct
     4261 gcatggccct gctatccctg cacctcttct acaaaaagtg cgcccagctg actgtgaacc
     4321 tgactcgatt cccggagact gtgcctcggg agctggttgt gcccgtggcc ggtagctgcg
     4381 tggtggatgc cgtccccgcc cctggcccca gccccagcct ctactgccgt gaggatggcc
     4441 agtgggccga acagccggtc acgggctgca gctgtgctcc ggggttcgag gcagctgagg
     4501 ggaacaccaa gtgccgagcc tgtgcccagg gcaccttcaa gcccctgtca ggagaagggt
     4561 cctgccagcc atgcccagcc aatagccact ctaacaccat tggatcagcc gtctgccagt
     4621 gccgcgtcgg gtacttccgg gcacgcacag acccccgggg tgcaccctgc accacccctc
     4681 cttcggctcc gcggagcgtg gtttcccgcc tgaacggctc ctccctgcac ctggaatgga
     4741 gtgcccccct ggagtctggt ggccgagagg acctcaccta cgccctccgc tgccgggagt
     4801 gccgacccgg aggctcctgt gcgccctgcg ggggagacct gacttttgac cccggccccc
     4861 gggacctggt ggagccctgg gtggtggttc gagggctacg tcctgacttc acctatacct
     4921 ttgaggtcac tgcattgaac ggggtatcct ccttagccac ggggcccgtc ccatttgagc
     4981 ctgtcaatgt caccactgac cgagaggtac ctcctgcagt gtccgacatc cgggtgacgc
     5041 ggtcctcacc cagcagcttg agcctggcct gggctgttcc ccgggcaccc agtggggctg
     5101 tgctggacta cgaggtcaaa taccatgaga agggcgccga gggtcccagc agcgtgcggt
     5161 tcctgaagac gtcagaaaac cgggcagagc tgcgggggct gaagcgggga gccagctacc
     5221 tggtgcaggt acgggcgcgc tctgaggccg gctacgggcc cttcggccag gaacatcaca
     5281 gccagaccca actggatgag agcgagggct ggcgggagca gctggccctg attgcgggca
     5341 cggcagtcgt gggtgtggtc ctggtcctgg tggtcattgt ggtcgcagtt ctctgcctca
     5401 ggaagcagag caatgggaga gaagcagaat attcggacaa acacggacag tatctcatcg
     5461 ggcatggtac taaggtctac atcgacccct tcacttatga agaccctaat gaggctgtga
     5521 gggaatttgc aaaagagatc gatgtctcct acgtcaagat tgaagaggtg attggtgcag
     5581 gtgagtttgg cgaggtgtgc cgggggcggc tcaaggcccc agggaagaag gagagctgtg
     5641 tggcaatcaa gaccctgaag ggtggctaca cggagcggca gcggcgtgag tttctgagcg
     5701 aggcctccat catgggccag ttcgagcacc ccaatatcat ccgcctggag ggcgtggtca
     5761 ccaacagcat gcccgtcatg attctcacag agttcatgga gaacggcgcc ctggactcct
     5821 tcctgcggct aaacgacgga cagttcacag tcatccagct cgtgggcatg ctgcggggca
     5881 tcgcctcggg catgcggtac cttgccgaga tgagctacgt ccaccgagac ctggctgctc
     5941 gcaacatcct agtcaacagc aacctcgtct gcaaagtgtc tgactttggc ctttcccgat
     6001 tcctggagga gaactcttcc gatcccacct acacgagctc cctgggagga aagattccca
     6061 tccgatggac tgccccggag gccattgcct tccggaagtt cacttccgcc agtgatgcct
     6121 ggagttacgg gattgtgatg tgggaggtga tgtcatttgg ggagaggccg tactgggaca
     6181 tgagcaatca ggacgtgatc aatgccattg aacaggacta ccggctgccc ccgcccccag
     6241 actgtcccac ctccctccac cagctcatgc tggactgttg gcagaaagac cggaatgccc
     6301 ggccccgctt cccccaggtg gtcagcgccc tggacaagat gatccggaac cccgccagcc
     6361 tcaaaatcgt ggcccgggag aatggcgggg cctcacaccc tctcctggac cagcggcagc
     6421 ctcactactc agcttttggc tctgtgggcg agtggcttcg ggccatcaaa atgggaagat
     6481 acgaagaaag tttcgcagcc gctggctttg gctccttcga gctggtcagc cagatctctg
     6541 ctgaggacct gctccgaatc ggagtcactc tggcgggaca ccagaagaaa atcttggcca
     6601 gtgtccagca catgaagtcc caggccaagc cgggaacccc gggtgggaca ggaggaccgg
     6661 ccccgcagta ctgacctgca ggaactcccc accccaggga caccgcctcc ccattttccg
     6721 gggcagagtg gggactcaca gaggccccca gccctgtgcc ccgctggatt gcactttgag
     6781 cccgtggggt gaggagttgg caatttggag agacaggatt tgggggttct gccataatag
     6841 gaggggaaaa tcacccccca gccacctcgg ggaactccag accaagggtg agggcgcctt
     6901 tccctcagga ctgggtgtga ccagaggaaa aggaagtgcc caacatctcc cagcctcccc
     6961 aggtgccccc ctcaccttga tgggtgcgtt cccgcagacc aaagagagtg tgactccctt
     7021 gccagctcca gagtgggggg gctgtcccag ggggcaagaa ggggtgtcag ggcccagtga
     7081 caaaatcatt ggggtttgta gtcccaactt gctgctgtca ccaccaaact caatcatttt
     7141 tttcccttgt aaatgcccct cccccagctg ctgccttcat attgaaggtt tttgagtttt
     7201 gtttttggtc ttaatttttc tccccgttcc ctttttgttt cttcgttttg tttttctacc
     7261 gtccttgtca taactttgtg ttggagggaa cctgtttcac tatggcctcc tttgcccaag
     7321 ttgaaacagg ggcccatcat catgtctgtt tccagaacag tgccttggtc atcccacatc
     7381 cccggacccc gcctgggacc cccaagctgt gtcctatgaa ggggtgtggg gtgaggtagt
     7441 gaaaagggcg gtagttggtg gtggaaccca gaaacggacg ccggtgcttg gaggggttct
     7501 taaattatat ttaaaaaagt aactttttgt ataaataaaa gaaaatggga cgtctcccag
     7561 ctccaggggt aaccggtgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     7621 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     7681 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7741 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7801 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7921 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7981 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     8041 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     8101 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     8161 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     8221 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     8281 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     8341 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     8401 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     8461 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     8521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     8641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     8761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10921 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    10981 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    11041 tcgctgagta gtgcgcgagc aaaatttaag ctacaa
//
