LOCUS       sequence-176452-        3650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab24 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..75
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     309..326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    443..464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(479..484,485..502,503..509)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    517..533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     522..544
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     541..557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     541..557
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        575..593
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     575..592
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1322..1331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1326..1335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1365..1384)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1366..1384)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1391..1408)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1391..1407)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1400..1422)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1548..2003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1635..1654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1845..1866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2081..2185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2186..2254,2255..3046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2404..2423)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3217..3650,1..155)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
       61 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      421 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      481 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      541 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
      601 agctatgcat ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa
      661 ttcactagtg attcaggtca gttatggcag cagttgtcaa aataagagtc cttcttttgc
      721 ccgagatcca cccccttctc ctcttgcatg aactctaaag cacagctact aaaatcttca
      781 gccaccttct gaaacacctc atcaacattt tttcctgttt tgctggacgt ctcgaaatgt
      841 tgtgcaccta tttcatctgc aaagtcttga acatcatggt agtctacttg ccgcgtgctt
      901 cggtccgcct caatcaggtc acttttggta ccacatagat atattttgca gtgctcctca
      961 cagttctgca actccttcac ccagaacctg gcccttccaa aactgctgct gtcagtcaag
     1021 tcatagcaga caatggcagc acgagctcct ctgtagtaaa ttctgctcat tgcttcataa
     1081 cgttcagacc cagcggtgtc ccatattccc agtgtaatca ccttctcacc aacattgagg
     1141 gcttttgcaa caaatgcagc ccctatagta ttttggtaag gtcccacaag aaagcgacga
     1201 tgtacatatc tctccaccag actggtttta ccaacgcttt ctttgccaag catgacaacc
     1261 ttagcgtcca ctcgcatggc agtcatgatg atatgggttt gtgcaaaatc gaattcccgc
     1321 ggccgccatg gcggccggga gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt
     1381 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1441 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1501 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     1561 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1621 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1681 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1741 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1801 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1861 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1921 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1981 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     2041 atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2101 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2161 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2221 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2281 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2341 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2401 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2461 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2521 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2581 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2641 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2701 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2761 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2821 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2881 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2941 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3001 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3061 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3121 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3181 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3241 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3301 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3361 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3421 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3481 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3541 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3601 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
//
