LOCUS       sequence-176459-        7729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localizes CRY2 to excitatory synapses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, 
            Dell'Acqua ML, Kennedy MJ
  TITLE     Optogenetic Control of Synaptic Composition and Function.
  JOURNAL   Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 
            10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26.
  PUBMED    28132827
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.12.037"; journalName: "Neuron"; date: 
            "2017-02-8- 8"; volume: "93"; issue: "3"; pages: "646-660.e5"
COMMENT     Sequence Label: CRY2PHR-PSD95FingR-GFP
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     291..313
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     375..394
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             431..1924
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2237..2260
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(2285..2287,2288..2995)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(2285..2287,2288..2995)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(2327..2348)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2588..2607)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2935..2956
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3041..3382
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             3431..3556
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          3605..3737
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3720..3739
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    3746..4334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3799..3819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4217..4228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4361..4378)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4367..4478
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4574..4594)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4574..4592)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4613..4629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4735..4754)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4800..4855
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4854..4873)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5216..5232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5240..5256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5264..5270,5271..5288,5289..5294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5309..5330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5389..5584
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5435..5570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5590..5724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5627..5646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5681..5700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5792..5809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5963..6551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6043..6062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6722..7513,7514..7582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7345..7364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7583..7687)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
       61 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
      121 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
      181 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
      241 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
      301 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
      361 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
      421 cggtaccacc atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat
      481 tgaggataat cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat
      541 ttggtgtcct gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa
      601 acaatcactt gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat
      661 caaaacccac aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa
      721 agtcgtcttt aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga
      781 gaagctggtg gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc
      841 gtgggagata tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa
      901 atgcttagat atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat
      961 aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga
     1021 ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc
     1081 tgataagtta ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa
     1141 gaaagttgtt gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag
     1201 cgtcagacac gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa
     1261 cagtgaagga gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc
     1321 tcggtatata tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg
     1381 gtttttccct tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg
     1441 ttatccgttg gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag
     1501 aataagagtg attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg
     1561 aatgaagtat ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg
     1621 gcagtatatc tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc
     1681 gttacaaggc gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct
     1741 tgcgagattg ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa
     1801 agcttctggt gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc
     1861 tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc
     1921 agcagatagt gctggtagtg ctggtagtgc tggtcccggg attacaatgc tcgaagtcaa
     1981 ggaagcatca ccaaccagca tccagatcag ctgggtgctc cacttgcgcc acgttcgcta
     2041 ctaccgcatc acctacggtg aaactggtgg caatagccct gtccaggaat tcaccgtgcc
     2101 tggcagcaag tccactgcta ccatcagcgg cctgaaacct ggtgtcgact ataccatcac
     2161 ggtgtacgcc gtcacgatct tcagcgccta ccgctccgcc tggccgccga tctccatcaa
     2221 ctaccgcacc ggaaccgatt acaaggatga cgacgataag ggtagcggct ccagtagatc
     2281 tggggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2341 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2401 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2461 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2521 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2581 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2641 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2701 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2761 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2821 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2881 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2941 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtactctag
     3001 gggagctggc gctggagcgg gtgcaggggc tggctctaga ttccagtgcc ggatctgcat
     3061 gcggaacttc agcgaccggt ccaacctgag caggcacatc agaacccaca ccggagaaaa
     3121 gcccttcgcc tgcgacattt gcggccggaa gttcgccatc agcagcaacc tgaacagcca
     3181 caccaagatc cacactggca gccagaaacc tttccagtgc agaatttgta tgagaaactt
     3241 tagcagaagc gacaacctgg ccagacacat ccggacacat actggtgaaa aaccttttgc
     3301 ctgtgatatc tgtggcagaa agtttgccac ctccggcaat ctgacccggc acacaaagat
     3361 tcacctgcgg ggcagccagc tatcgattgt cgacgctcct gaacaacgtg aaggtgcttc
     3421 tcaagtttct gtttctgtta cttttgaaga tgttgctgtt ctttttactc gtgatgaatg
     3481 gaaaaaactt gatctttctc aacgttctct ttatcgtgaa gttatgcttg aaaattattc
     3541 taatcttgct tctatggctt aaacgcgttg tacaagtaaa gcggccccgg actcgaggcc
     3601 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
     3661 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     3721 tgcctttctc tccacaggtg tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3781 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3841 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3901 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3961 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4021 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4081 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4141 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4201 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4261 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4321 ggccgcctcc ccgcctggaa ttcgagctcg gtacgatcag cctcgactgt gccttctagt
     4381 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4441 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4501 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4561 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     4621 tgtttcctgt gtgaaattgt tatccgctaa ttcactcctc aggtgcaggc tgcctatcag
     4681 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tcgatctttt
     4741 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     4801 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     4861 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     4921 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     4981 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     5041 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     5101 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     5161 ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat
     5221 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5281 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5341 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta
     5401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5581 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5701 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     5761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7681 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattat
//
