LOCUS       sequence-176467-        3643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab9b cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..57
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     291..308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    425..446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(461..466,467..484,485..491)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    499..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     504..526
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     523..539
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     523..539
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        557..575
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     557..574
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1297..1306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1301..1310
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1340..1359)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1341..1359)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1366..1383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1366..1382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1375..1397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1523..1978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1610..1629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1820..1841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2056..2160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2161..2229,2230..3021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2379..2398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3192..3643,1..137)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
       61 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      301 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      361 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      421 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      481 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      541 atgattacgc caagctattt aggtgacact atagaatact caagctatgc atccaacgcg
      601 ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag tgattttaga
      661 ggcacctcaa cagcaggacg agcgggtcac tttacggttt ccatggagat cgatggaact
      721 gctcagcaag gtgtggtcaa tggggtcctc gctagccaga acctcccgaa cagcggcttc
      781 gaaagcagcc cccacatttg tgtcgtcctt tgcactggtt tcaaagtacg gacagcagcc
      841 attttcttca caccaggccc tggcctcatc ctcaccgacc tcacgctctt ccttgtctac
      901 cttgtttccc agcactacaa aggggaagcg ctccgggtct cgtacatctg aataatacat
      961 gaactccttt ttccagcagc ccagattctg gaagctctgc aggtcatcca ctgcgaacgt
     1021 gagcagacag caatctgcac cacggtaaaa aggtgtgcgc aggctcttga atcgctcctg
     1081 gcctgccgtg tcccagatct gtagggtgac cagccgacca tcgatttcga ggtcgcggtt
     1141 aaggaattcg acaccgatgg tatggaaaga ctgcgagtcg aagcggtccg tcacatatcg
     1201 gttcatgagg gagcttttgc ccacgccgcc atcccccaga agaatgacct tcagcaagag
     1261 gctcttccca ctcataacgg caatcgaatt cccgcggccg ccatggcggc cgggagcatg
     1321 cgacgtcggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     1381 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     1441 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     1501 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1681 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1861 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1921 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     1981 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
     2041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2161 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2221 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2281 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2341 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2401 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2461 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2521 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2581 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2641 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2701 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2761 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2881 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2941 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gca
//
