LOCUS       sequence-176481-       12979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with an internal PreScission 
            protease site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 12979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3400  (YCp LEU2 Rpb1 A1529_G1534delInsLEVLF...
FEATURES             Location/Qualifiers
     source          1..12979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..504)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(186..207)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     398..417
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     634..656
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     648..665
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     649..665
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     672..691
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        672..690
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6882..6905
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(7780..7798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7819..7835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7819..7835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7832..7854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7843..7859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7867..7873,7874..7891,7892..7897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8050..8067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8221..8809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8301..8320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8980..9771,9772..9840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9603..9622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9841..9945)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9982..10485
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10527..10545
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10583..10605)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10705..10724
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(11217..12311)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(12324..12728)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg
       61 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      121 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      181 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      241 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      301 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      361 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      421 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      481 ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg
      541 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      601 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      661 ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctcttggga agcttcagta
      721 ccgcgatttt tcagctaatg gatcagatga tatataaaca aaaagtggga tacagcatat
      781 cgacgcctcc actacaaatt aacagaagga tgaaaaccaa taagaaaatt tctaaaagaa
      841 gaagcctgaa aaatctccat ggtgccttaa aaggactatt gaaagagtcc ggcaagaaga
      901 gtgagtccaa aatacgaaaa catagcgatt gcaatcctgt tcatcgagta tatccgccaa
      961 atatcgaaaa acggaagacc aaaaaggacg atggaatttc acggccaatt gctgaaagaa
     1021 atggtcatgt gtatataatg tccaaggaaa accacatcat ccctaaacta accgatgatg
     1081 aagtcatgga gcgccacaaa ctagcagacg aaaacatgag aaaagtgtgg tccaacatca
     1141 taagcaaata cgagtctatt gaagaacaag gagacctcgt agacctgaaa actggtgaaa
     1201 tagttgaaga taatgggcat atcaagacgc tgacggccaa caacagcacg aaagataaaa
     1261 gaacaaaata cacaagcgtg ctcagagaca ttatcgacat cagcgacgag gaagatggtg
     1321 ataaaaatga tgaatatact ctttgggcta acgatagcga ggcaagcgat tcagaggtgg
     1381 atgctgacaa cgacactgaa gaggaaaaag acgagaaatt gatagacgca gatttcaaga
     1441 agtacgaggc caaactctcg aaaaggatat tacgagatta aggtgctaaa atgccgtata
     1501 ttcgaatttc attaccatca gaatatatat atatatatat gagtctatat tagggactat
     1561 catacggtaa cccggacctc cattacccgc atgactttac tcactaaaga cgattgtcgg
     1621 ttttaaacat tttttttttt tcatttcgac cgtcggttct ccttagtttg ttcagtggca
     1681 acaagtggac aaacaagaag agcgtgcagc accattcacc gccagctttg tatcgtcctt
     1741 tagagtgcag caagaacatt aattgctctc acagaggtat cataagaaca tccgattcgt
     1801 tcattaactg aaatcatatc tgtgttgttc tgatcgactc tctttgttag ttttatcttc
     1861 catttttttt tattcctttc attttccaat aaaatttgaa caggatcgat ataaacagat
     1921 aaaaaccctc caaagttaac catttgttta tatttgcttt tctgcataag ttttcctagc
     1981 gaattccctg atcaactttc aaggaaaact aaaactactg tattataaga ggtttttttt
     2041 ttcattccag attaattttg aaatacgata tcctcaagtt tatctaccag aatatttgac
     2101 taagaaatca aactctgtta ataataatat aattataaaa acctcaacta gaaactccaa
     2161 aaaaaaaaat ttaccatttt ttactttcta tccttgttaa ccaaatttca aaaaaatttt
     2221 accttttctt tttccagaag agggaccaat cataaagata gcaataacac tttaccccaa
     2281 aatataaatc agacatggta ggacaacagt attctagtgc tccactccgt acagtaaaag
     2341 aggtccaatt cggtcttttc tcacctgaag aagttagagc aatcagtgtg gccaaaatta
     2401 gatttccaga gacaatggat gaaacccaga cgagagcgaa aattggtggt ctaaacgacc
     2461 ctaggttagg ctctattgat cgtaatctga agtgtcaaac ttgtcaagag ggtatgaacg
     2521 aatgtcctgg tcattttggt cacatagatt tagcaaaacc tgtatttcat gttggtttta
     2581 ttgccaaaat taagaaagta tgtgagtgtg tctgtatgca ctgtggtaag ctattactgg
     2641 atgaacataa tgaattaatg agacaagctc tagcaatcaa agacagtaaa aaaaggtttg
     2701 ctgcaatttg gactttatgt aaaacaaaaa tggtctgcga aacagatgtc ccttctgaag
     2761 atgatcctac tcagctcgta tcaaggggag gttgtggtaa tacacagcct acaattcgta
     2821 aggatgggtt gaaattagtt ggtagttgga aaaaagatag agccacgggg gatgcggatg
     2881 aaccagaact aagagtttta agtacggagg aaatcttgaa tatttttaag catatctcag
     2941 taaaagactt cactagtttg ggtttcaacg aagttttttc tcgtccagaa tggatgattt
     3001 taacatgcct tcctgtccca ccaccaccgg tgcgtccatc catttccttc aatgaatctc
     3061 aaagaggtga ggatgattta acctttaaac ttgctgatat tttaaaagct aatattagtt
     3121 tggaaacact agagcataac ggtgctccac atcatgctat tgaagaagca gagagtttat
     3181 tacaatttca tgttgccact tatatggata atgatattgc tggtcaacca caagctctgc
     3241 agaagtccgg ccgtcccgtt aaatctattc gtgctcgttt gaagggtaaa gagggtcgta
     3301 tcagaggtaa tttaatgggt aagcgtgtgg atttttcggc aagaactgtt atttctggtg
     3361 atcctaattt ggaattagac caagtcggtg ttccaaaatc tattgccaag actttaacat
     3421 acccagaagt ggtcacacca tataacatag atcgtctgac gcaacttgtt aggaatggac
     3481 caaatgagca ccccggtgcc aaatacgtca ttcgtgatag cggagaccgt atagatttaa
     3541 gatacagtaa aagggcaggt gatattcaat tacagtatgg gtggaaagtt gaacgtcata
     3601 ttatggacaa tgatccagtt ttattcaacc gtcaaccttc gttgcacaaa atgtccatga
     3661 tggcccacag agtaaaagtt attccatatt ctacatttag attgaatttg tccgttacat
     3721 ctccatacaa tgccgatttc gacggtgacg aaatgaatct tcacgttcct cagtctgagg
     3781 aaacaagggc ggaactttct caattatgtg ctgttcctct gcaaattgtt tcaccacaat
     3841 ctaacaaacc ttgtatgggt attgttcaag atactttgtg tggtattcgt aaactgacat
     3901 taagagatac atttatagaa cttgatcaag ttttgaatat gctttattgg gttccagatt
     3961 gggatggtgt tattccgaca cctgcaatta tcaagcccaa acctttgtgg tccggtaaac
     4021 aaatcttgtc tgtggctatc ccaaacggta ttcatttaca acgttttgat gagggcacta
     4081 ctctgctttc tccaaaggat aatggtatgc ttattattga cggtcaaatc atttttggtg
     4141 tagtagagaa aaaaaccgtt ggatcctcca atggtggttt aattcatgtt gttacgagag
     4201 aaaagggacc tcaagtttgt gctaagttgt ttggtaacat acagaaagtt gttaactttt
     4261 ggttactaca taatgggttt tcaacaggta ttggtgatac cattgcggac ggcccaacaa
     4321 tgagggaaat tacagagaca attgcagagg ctaaaaagaa agttttggat gttacgaaag
     4381 aagcccaggc aaacttattg actgctaaac atggtatgac tctccgtgag tcttttgagg
     4441 ataacgttgt tcggttccta aatgaagcaa gagataaggc aggtcgttta gctgaagtca
     4501 atttgaaaga tttgaacaat gtgaaacaaa tggttatggc aggttccaag ggttcattta
     4561 ttaatatcgc gcaaatgtca gcttgtgtag gacagcaatc tgttgaaggt aaacgtattg
     4621 cttttgggtt cgttgatcgt accttacctc atttctctaa agatgattac tccccagagt
     4681 ctaaaggttt tgttgagaac tcatatttga gaggtttgac cccacaagaa ttttttttcc
     4741 atgcaatggg tggtcgtgaa ggtcttatcg ataccgccgt caaaacagcc gaaacaggtt
     4801 atattcaacg tcgtttagtg aaagctctag aagatatcat ggttcattac gataacacca
     4861 caagaaactc attgggtaac gttattcagt ttatttatgg tgaagatggt atggatgctg
     4921 cgcatattga aaagcaatcg ctagatacta ttggtggctc cgatgcagct tttgaaaaga
     4981 gatacagagt tgatttattg aatacagacc atacccttga tccctcacta ttggaatccg
     5041 gatctgagat acttggcgat ttgaaacttc aagttctcct ggatgaagaa tacaaacaat
     5101 tagtgaaaga tcgtaaattt ttgagggaag tttttgttga tggtgaagca aactggccat
     5161 taccagtcaa cataagacgt attattcaaa atgctcaaca aactttccac atagatcata
     5221 cgaaaccatc tgatttaaca atcaaagaca tcgttcttgg tgtaaaggat ttgcaagaaa
     5281 acttattagt gttgcgtggt aagaatgaaa ttatacaaaa tgcccagcga gatgcagtta
     5341 cattgttctg ctgtttatta cgttcccgtt tggccacacg tagagttcta caagagtaca
     5401 gactaacaaa acaggcattc gattgggtat taagtaatat cgaggcacaa ttcctccgtt
     5461 ctgttgttca ccctggtgaa atggttggtg ttctagcagc ccaatccatt ggtgaaccag
     5521 ccacacaaat gacccttaac accttccatt ttgctggtgt tgcttccaaa aaagttactt
     5581 ctggtgtccc ccgtttaaag gaaattttga atgtggccaa aaacatgaaa accccttcct
     5641 tgactgtata cttagagccc gggcatgctg ccgatcaaga acaagcgaag ttgatcagat
     5701 ctgctatcga gcataccact ttaaagagtg tcactattgc ttcagaaatt tactatgatc
     5761 ctgatccacg ttccacagtt attccagaag atgaagaaat tatccaactt catttctcat
     5821 tattggatga agaggctgaa caatcttttg accaacaatc accttggtta ttacgtctgg
     5881 aactggatcg tgcagcaatg aatgataaag acttaacaat gggtcaggtt ggtgaaagaa
     5941 tcaagcaaac attcaaaaat gatttgtttg ttatctggtc tgaagacaac gatgagaagt
     6001 tgatcatccg ttgtcgtgtt gttcgtccaa agtcactaga tgctgagact gaagcagaag
     6061 aagatcatat gttgaagaaa attgagaaca caatgttaga gaatattaca ttacgtggtg
     6121 tagagaacat cgagcgtgtt gtcatgatga aatatgaccg taaagtacca agtccaactg
     6181 gtgaatacgt taaggaacct gaatgggtgt tggaaacaga tggtgttaac ttatctgaag
     6241 ttatgactgt tcctggtatc gacccaacca gaatctatac caactccttc attgatataa
     6301 tggaagttct aggtattgaa gctggtcgtg cagccttgta taaagaagtt tacaatgtta
     6361 ttgcttctga tggttcgtat gttaactacc gtcatatggc tttgttagtc gatgttatga
     6421 caacccaagg tggcttaact tctgttactc gtcatggttt caacagatca aatacaggtg
     6481 ccttaatgag atgttcattt gaagaaactg tcgaaatttt gtttgaagct ggtgcttcag
     6541 ccgaattaga tgattgtcgt ggtgtttcgg aaaatgtcat tcttggtcaa atggctccaa
     6601 tcggtaccgg tgcatttgat gtgatgatcg atgaggagtc actggtaaaa tacatgccag
     6661 aacaaaaaat aactgagatt gaagacggac aagatggtgg cgtcacacca tacagtaacg
     6721 aaagtggttt ggtcaatgca gatcttgacg ttaaagatga gctaatgttt tcacctctgg
     6781 ttgattcggg ttcaaatgac gctatggctg gaggatttac agcgtacggt ggtgctgatt
     6841 atggtgaagc cacgtctcca tttggtgctt atggtgaagc actggaagtt ctgttccagg
     6901 ggccctttgg agtctcctca ccaggctttt ctccaacttc cccaacatac tctcctacct
     6961 ctccagcgta ctcaccaaca tcaccatcgt actcaccaac atcaccatcg tactcgccaa
     7021 catcaccatc gtactcacct acatcaccat cgtattcacc aacgtcacca tcatattcgc
     7081 caacgtcacc atcatattcg ccaacgtcgc catcgtattc tccaacgtca ccatcgtatt
     7141 cgccaacgtc gccttcctac tctcccacgt cgccaagcta cagccctacg tctccttctt
     7201 attctcctac atctccatca tactctccta cgtcaccaag ttacagccca acgtcaccaa
     7261 gttacagccc aacgtctcca gcctattccc caacatcacc aagttatagt cctacatcgc
     7321 cttcatactc tccaacatca ccatcctatt ccccaacatc accttcttac tctcccacct
     7381 ctccaaacta tagccctact tcaccttctt actccccaac atctccaggc tacagcccag
     7441 gatctcctgc atattctcca aagcaagacg aacaaaagca taatgaaaat gaaaattcca
     7501 gatgatatag tatatcatcc ttacgtattt gacgttatta cattatatat agtttctcaa
     7561 ataatatttc tagtttattt ttgtatcata ataaaaacgt ataccaaata taccattatt
     7621 tttcatagca ttatggtagg gatagggaat caagtaacta atttatatcc gcagagcatt
     7681 gggaaaacca acggcgctag taaatgcatt taaattacgt ccgtccaact tctaagctta
     7741 agggtttctc gagggggggc ccggtaccag cttttgttcc ctttagtgag ggttaattcc
     7801 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7861 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7921 gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7981 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8101 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8641 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9121 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9961 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
    10021 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
    10081 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
    10141 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
    10201 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
    10261 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
    10321 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
    10381 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
    10441 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc taagaaacca
    10501 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10561 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10621 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10681 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10741 tcgactacgt cgtaaggccg tttctgacag agtaaaattc ttgagggaac tttcaccatt
    10801 atgggaaatg gttcaagaag gtattgactt aaactccatc aaatggtcag gtcattgagt
    10861 gttttttatt tgttgtattt tttttttttt agagaaaatc ctccaatatc aaattaggaa
    10921 tcgtagtttc atgattttct gttacaccta actttttgtg tggtgccctc ctccttgtca
    10981 atattaatgt taaagtgcaa ttctttttcc ttatcacgtt gagccattag tatcaatttg
    11041 cttacctgta ttcctttact atcctccttt ttctccttct tgataaatgt atgtagattg
    11101 cgtatatagt ttcgtctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta
    11161 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
    11221 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
    11281 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
    11341 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
    11401 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccgtg gcatggttcg
    11461 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
    11521 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
    11581 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
    11641 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
    11701 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
    11761 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
    11821 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
    11881 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
    11941 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
    12001 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
    12061 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
    12121 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
    12181 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
    12241 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
    12301 ggggcagaca taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc
    12361 tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa
    12421 aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc
    12481 atgaacgctt ctctattcta tatgaaaagc cggttccggc ctctcacctt tcctttttct
    12541 cccaattttt cagttgaaaa aggtatatgc gtcaggcgac ctctgaaatt aacaaaaaat
    12601 ttccagtcat cgaatttgat tctgtgcgat agcgcccctg tgtgttctcg ttatgttgag
    12661 gaaaaaaata atggttgcta agagattcga actcttgcat cttacgatac ctgagtattc
    12721 ccacagttaa ctgcggtcaa gatatttctt gaatcaggcg ccttagaccg ctcggccaaa
    12781 caaccaatta cttgttgaga aatagagtat aattatccta taaatataac gtttttgaac
    12841 acacatgaac aaggaagtac aggacaattg attttgaaga gaatgtggat tttgatgtaa
    12901 ttgttgggat tccattttta ataaggcaat aatattaggt atgtggatat actagaagtt
    12961 ctcctcgacc gtcgatatg
//
